An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection

This paper presents a novel method of fabricating an enzymatic biosensor for breath analysis using chromatography paper as enzyme supporting layer and a liquid phase layer on top of screen printed carbon electrodes. We evaluated the performance with ethanol vapor being one of the breathing ingredien...

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Main Authors: Tatsumi Kuretake, Shogo Kawahara, Masanobu Motooka, Shigeyasu Uno
Format: Article
Language:English
Published: MDPI AG 2017-02-01
Series:Sensors
Subjects:
Online Access:http://www.mdpi.com/1424-8220/17/2/281
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author Tatsumi Kuretake
Shogo Kawahara
Masanobu Motooka
Shigeyasu Uno
author_facet Tatsumi Kuretake
Shogo Kawahara
Masanobu Motooka
Shigeyasu Uno
author_sort Tatsumi Kuretake
collection DOAJ
description This paper presents a novel method of fabricating an enzymatic biosensor for breath analysis using chromatography paper as enzyme supporting layer and a liquid phase layer on top of screen printed carbon electrodes. We evaluated the performance with ethanol vapor being one of the breathing ingredients. The experimental results show that our sensor is able to measure the concentration of ethanol vapor within the range of 50 to 500 ppm. These results suggest the ability of detecting breath ethanol, and it can possibly be applied as a generic vapor biosensor to a wide range of diseases.
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spelling doaj.art-fc42b2c9512b4c20903d3c54f86446412022-12-22T02:20:10ZengMDPI AGSensors1424-82202017-02-0117228110.3390/s17020281s17020281An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor DetectionTatsumi Kuretake0Shogo Kawahara1Masanobu Motooka2Shigeyasu Uno3Department of Electrical and Electronic Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, JapanDepartment of Electrical and Electronic Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, JapanDepartment of Electrical and Electronic Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, JapanDepartment of Electrical and Electronic Engineering, Ritsumeikan University, Kusatsu, Shiga 525-8577, JapanThis paper presents a novel method of fabricating an enzymatic biosensor for breath analysis using chromatography paper as enzyme supporting layer and a liquid phase layer on top of screen printed carbon electrodes. We evaluated the performance with ethanol vapor being one of the breathing ingredients. The experimental results show that our sensor is able to measure the concentration of ethanol vapor within the range of 50 to 500 ppm. These results suggest the ability of detecting breath ethanol, and it can possibly be applied as a generic vapor biosensor to a wide range of diseases.http://www.mdpi.com/1424-8220/17/2/281electrochemical sensorchromatography paper-based sensorgas sensor
spellingShingle Tatsumi Kuretake
Shogo Kawahara
Masanobu Motooka
Shigeyasu Uno
An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
Sensors
electrochemical sensor
chromatography paper-based sensor
gas sensor
title An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
title_full An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
title_fullStr An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
title_full_unstemmed An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
title_short An Electrochemical Gas Biosensor Based on Enzymes Immobilized on Chromatography Paper for Ethanol Vapor Detection
title_sort electrochemical gas biosensor based on enzymes immobilized on chromatography paper for ethanol vapor detection
topic electrochemical sensor
chromatography paper-based sensor
gas sensor
url http://www.mdpi.com/1424-8220/17/2/281
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