Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia Inhibition
This study aimed to investigate the role of the bioaugmented critical biomass that should be injected for successful bioaugmentation for addressing ammonia inhibition in anaerobic reactors used for biogas production. Cattle manure was used as a feedstock for anaerobic digestion (AD). A mixed microbi...
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MDPI AG
2023-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/7/1710 |
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| author | Sotirios D. Kalamaras Maria Lida Christou Christos A. Tzenos Sotirios Vasileiadis Dimitrios G. Karpouzas Thomas A. Kotsopoulos |
| author_facet | Sotirios D. Kalamaras Maria Lida Christou Christos A. Tzenos Sotirios Vasileiadis Dimitrios G. Karpouzas Thomas A. Kotsopoulos |
| author_sort | Sotirios D. Kalamaras |
| collection | DOAJ |
| description | This study aimed to investigate the role of the bioaugmented critical biomass that should be injected for successful bioaugmentation for addressing ammonia inhibition in anaerobic reactors used for biogas production. Cattle manure was used as a feedstock for anaerobic digestion (AD). A mixed microbial culture was acclimated to high concentrations of ammonia and used as a bioaugmented culture. Different volumes of bioaugmented culture were injected in batch anaerobic reactors under ammonia toxicity levels i.e., 4 g of NH<sub>4</sub><sup>+</sup>-N L<sup>−1</sup>. The results showed that injecting a volume equal to 65.62% of the total working reactor volume yielded the best methane production. Specifically, this volume of bioaugmented culture resulted in methane production rates of 196.18 mL g<sup>−1</sup> Volatile Solids (VS) and 245.88 mL g<sup>−1</sup> VS after 30 and 60 days of AD, respectively. These rates were not significantly different from the control reactors (30d: 205.94 mL CH<sub>4</sub> g<sup>−1</sup> VS and 60d: 230.26 mL CH<sub>4</sub> g<sup>−1</sup> VS) operating without ammonia toxicity. Analysis of the microbial community using 16S rRNA gene sequencing revealed the dominance of acetoclastic methanogen members from the genus <i>Methanosaeta</i> in all reactors. |
| first_indexed | 2024-03-11T00:49:25Z |
| format | Article |
| id | doaj.art-fc43d97fe5524da982b725c97ce7626e |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T00:49:25Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-fc43d97fe5524da982b725c97ce7626e2023-11-18T20:35:24ZengMDPI AGMicroorganisms2076-26072023-06-01117171010.3390/microorganisms11071710Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia InhibitionSotirios D. Kalamaras0Maria Lida Christou1Christos A. Tzenos2Sotirios Vasileiadis3Dimitrios G. Karpouzas4Thomas A. Kotsopoulos5Department of Hydraulics, Soil Science and Agricultural Engineering, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Hydraulics, Soil Science and Agricultural Engineering, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Hydraulics, Soil Science and Agricultural Engineering, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, GreeceDepartment of Biochemistry and Biotechnology, University of Thessaly, 41500 Larissa, GreeceDepartment of Hydraulics, Soil Science and Agricultural Engineering, School of Agriculture, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceThis study aimed to investigate the role of the bioaugmented critical biomass that should be injected for successful bioaugmentation for addressing ammonia inhibition in anaerobic reactors used for biogas production. Cattle manure was used as a feedstock for anaerobic digestion (AD). A mixed microbial culture was acclimated to high concentrations of ammonia and used as a bioaugmented culture. Different volumes of bioaugmented culture were injected in batch anaerobic reactors under ammonia toxicity levels i.e., 4 g of NH<sub>4</sub><sup>+</sup>-N L<sup>−1</sup>. The results showed that injecting a volume equal to 65.62% of the total working reactor volume yielded the best methane production. Specifically, this volume of bioaugmented culture resulted in methane production rates of 196.18 mL g<sup>−1</sup> Volatile Solids (VS) and 245.88 mL g<sup>−1</sup> VS after 30 and 60 days of AD, respectively. These rates were not significantly different from the control reactors (30d: 205.94 mL CH<sub>4</sub> g<sup>−1</sup> VS and 60d: 230.26 mL CH<sub>4</sub> g<sup>−1</sup> VS) operating without ammonia toxicity. Analysis of the microbial community using 16S rRNA gene sequencing revealed the dominance of acetoclastic methanogen members from the genus <i>Methanosaeta</i> in all reactors.https://www.mdpi.com/2076-2607/11/7/1710anaerobic digestionbiogasmethanebioaugmentationcritical biomass |
| spellingShingle | Sotirios D. Kalamaras Maria Lida Christou Christos A. Tzenos Sotirios Vasileiadis Dimitrios G. Karpouzas Thomas A. Kotsopoulos Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia Inhibition Microorganisms anaerobic digestion biogas methane bioaugmentation critical biomass |
| title | Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia Inhibition |
| title_full | Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia Inhibition |
| title_fullStr | Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia Inhibition |
| title_full_unstemmed | Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia Inhibition |
| title_short | Investigation of the Critical Biomass of Acclimated Microbial Communities to High Ammonia Concentrations for a Successful Bioaugmentation of Biogas Anaerobic Reactors with Ammonia Inhibition |
| title_sort | investigation of the critical biomass of acclimated microbial communities to high ammonia concentrations for a successful bioaugmentation of biogas anaerobic reactors with ammonia inhibition |
| topic | anaerobic digestion biogas methane bioaugmentation critical biomass |
| url | https://www.mdpi.com/2076-2607/11/7/1710 |
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