MDA5 plays a crucial role in enterovirus 71 RNA-mediated IRF3 activation.
Induction of type-I interferons (IFNs), IFN-α/β, is crucial to innate immunity against RNA virus infection. Cytoplasmic retinoic acid-inducible gene I (RIG-I)-like receptors, including RIG-I and melanoma differentiation-associated gene 5 (MDA5), are critical pathogen sensors for activation of type-I...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3641126?pdf=render |
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| author | Rei-Lin Kuo Li-Ting Kao Sue-Jane Lin Robert Yung-Liang Wang Shin-Ru Shih |
| author_facet | Rei-Lin Kuo Li-Ting Kao Sue-Jane Lin Robert Yung-Liang Wang Shin-Ru Shih |
| author_sort | Rei-Lin Kuo |
| collection | DOAJ |
| description | Induction of type-I interferons (IFNs), IFN-α/β, is crucial to innate immunity against RNA virus infection. Cytoplasmic retinoic acid-inducible gene I (RIG-I)-like receptors, including RIG-I and melanoma differentiation-associated gene 5 (MDA5), are critical pathogen sensors for activation of type-I IFN expression in response to RNA virus infection. MDA5 is required for type-I IFN expression in mouse models in response to infection by picornaviruses, such as encephalomyocarditis virus (EMCV) and coxsackievirus B3. Enterovirus 71 (EV71) belongs to picornaviridae and contains positive-stranded RNA genome that is linked with VPg protein at the 5' end. Although a recent study showed that EV71 3C protease could suppress RIG-I-mediated IFN-β response, the cytoplasmic RIG-I-like receptor that is directly involved in the recognition of EV71 RNA remains unclear. Using EV71-derived RNA as an agonist, we demonstrate that MDA5 is involved in EV71 RNA-mediated IRF3 activation and IFN-β transcription. Our data also show that overexpression of the MDA5 protein reverses the suppression of IRF3 activation caused by EV71 infection. These results indicate that MDA5 is an important factor for EV71 RNA-activated type-I IFN expression. Furthermore, we also show that EV71 infection enhances MDA5 degradation and that the degradation could be inhibited by a broad spectrum caspase inhibitor. |
| first_indexed | 2024-12-10T18:54:01Z |
| format | Article |
| id | doaj.art-fc46e0462279436d956a0f42196147a0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T18:54:01Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fc46e0462279436d956a0f42196147a02022-12-22T01:37:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6343110.1371/journal.pone.0063431MDA5 plays a crucial role in enterovirus 71 RNA-mediated IRF3 activation.Rei-Lin KuoLi-Ting KaoSue-Jane LinRobert Yung-Liang WangShin-Ru ShihInduction of type-I interferons (IFNs), IFN-α/β, is crucial to innate immunity against RNA virus infection. Cytoplasmic retinoic acid-inducible gene I (RIG-I)-like receptors, including RIG-I and melanoma differentiation-associated gene 5 (MDA5), are critical pathogen sensors for activation of type-I IFN expression in response to RNA virus infection. MDA5 is required for type-I IFN expression in mouse models in response to infection by picornaviruses, such as encephalomyocarditis virus (EMCV) and coxsackievirus B3. Enterovirus 71 (EV71) belongs to picornaviridae and contains positive-stranded RNA genome that is linked with VPg protein at the 5' end. Although a recent study showed that EV71 3C protease could suppress RIG-I-mediated IFN-β response, the cytoplasmic RIG-I-like receptor that is directly involved in the recognition of EV71 RNA remains unclear. Using EV71-derived RNA as an agonist, we demonstrate that MDA5 is involved in EV71 RNA-mediated IRF3 activation and IFN-β transcription. Our data also show that overexpression of the MDA5 protein reverses the suppression of IRF3 activation caused by EV71 infection. These results indicate that MDA5 is an important factor for EV71 RNA-activated type-I IFN expression. Furthermore, we also show that EV71 infection enhances MDA5 degradation and that the degradation could be inhibited by a broad spectrum caspase inhibitor.http://europepmc.org/articles/PMC3641126?pdf=render |
| spellingShingle | Rei-Lin Kuo Li-Ting Kao Sue-Jane Lin Robert Yung-Liang Wang Shin-Ru Shih MDA5 plays a crucial role in enterovirus 71 RNA-mediated IRF3 activation. PLoS ONE |
| title | MDA5 plays a crucial role in enterovirus 71 RNA-mediated IRF3 activation. |
| title_full | MDA5 plays a crucial role in enterovirus 71 RNA-mediated IRF3 activation. |
| title_fullStr | MDA5 plays a crucial role in enterovirus 71 RNA-mediated IRF3 activation. |
| title_full_unstemmed | MDA5 plays a crucial role in enterovirus 71 RNA-mediated IRF3 activation. |
| title_short | MDA5 plays a crucial role in enterovirus 71 RNA-mediated IRF3 activation. |
| title_sort | mda5 plays a crucial role in enterovirus 71 rna mediated irf3 activation |
| url | http://europepmc.org/articles/PMC3641126?pdf=render |
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