CsCLE3 delays female flower anthesis in cucumber
A corolla opening is a necessary process affecting the quality of fruits and market competitiveness in cucumber ( Cucumis sativus ). In the previous paper, we identified a unique cucumber line (‘6457’) that possesses extra-long ovaries and shows a delayed corolla opening when nutrient supplies are a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2023-03-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://www.journalssystem.com/asbp/-CsCLE3-delays-female-flower-anthesis-in-ncucumber,162334,0,2.html |
| _version_ | 1797266043590148096 |
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| author | Mingyue Lei Xiaofei Song Jinshuang Zheng Chengzhen Sun Liying Yan |
| author_facet | Mingyue Lei Xiaofei Song Jinshuang Zheng Chengzhen Sun Liying Yan |
| author_sort | Mingyue Lei |
| collection | DOAJ |
| description | A corolla opening is a necessary process affecting the quality of fruits and market competitiveness in cucumber ( Cucumis sativus ). In the previous paper, we identified a unique cucumber line (‘6457’) that possesses extra-long ovaries and shows a delayed corolla opening when nutrient supplies are abundant. We also previously showed that the expression of CsCLE3 ( Csa4G627800 ) is correlated with the delayed opening of the female corolla. Here, we investigated the function of CsCLE3 in cucumber by conducting transgenic experiments and phenotypic analysis. The results showed that the expression of CsCLE3 in the extra-long ovary was significantly lower than in the typical ovary. In CsCLE3 -overexpressed plants, the capacity to produce extra-long ovaries was lost, and the average rates of the extra-long ovary and the extra-long ovary plant were both 0%. In the CsCLE3 knockout plants obtained by the CRISPR/Cas9 system, the average extra-long ovary and extra-long ovary plant rates were significantly higher- 66.67% and 100%, respectively. Our study proved a negative regulating corolla opening time factor and provides new insight into the molecular basis of cucumber reproduction, producing fruits with flowers remaining on the tip. |
| first_indexed | 2024-04-25T00:54:25Z |
| format | Article |
| id | doaj.art-fc4709c404214d77977d178f166989c0 |
| institution | Directory Open Access Journal |
| issn | 0001-6977 2083-9480 |
| language | English |
| last_indexed | 2024-04-25T00:54:25Z |
| publishDate | 2023-03-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-fc4709c404214d77977d178f166989c02024-03-11T12:57:59ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae0001-69772083-94802023-03-019211710.5586/asbp.9204162334CsCLE3 delays female flower anthesis in cucumberMingyue Lei0Xiaofei Song1Jinshuang Zheng2Chengzhen Sun3https://orcid.org/0000-0003-0770-6877Liying Yan4Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, ChinaHebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, ChinaHebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, ChinaHebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, ChinaHebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, ChinaA corolla opening is a necessary process affecting the quality of fruits and market competitiveness in cucumber ( Cucumis sativus ). In the previous paper, we identified a unique cucumber line (‘6457’) that possesses extra-long ovaries and shows a delayed corolla opening when nutrient supplies are abundant. We also previously showed that the expression of CsCLE3 ( Csa4G627800 ) is correlated with the delayed opening of the female corolla. Here, we investigated the function of CsCLE3 in cucumber by conducting transgenic experiments and phenotypic analysis. The results showed that the expression of CsCLE3 in the extra-long ovary was significantly lower than in the typical ovary. In CsCLE3 -overexpressed plants, the capacity to produce extra-long ovaries was lost, and the average rates of the extra-long ovary and the extra-long ovary plant were both 0%. In the CsCLE3 knockout plants obtained by the CRISPR/Cas9 system, the average extra-long ovary and extra-long ovary plant rates were significantly higher- 66.67% and 100%, respectively. Our study proved a negative regulating corolla opening time factor and provides new insight into the molecular basis of cucumber reproduction, producing fruits with flowers remaining on the tip.https://www.journalssystem.com/asbp/-CsCLE3-delays-female-flower-anthesis-in-ncucumber,162334,0,2.htmlcscle3corolla openingcucumis sativusgene functionextra-long ovary |
| spellingShingle | Mingyue Lei Xiaofei Song Jinshuang Zheng Chengzhen Sun Liying Yan CsCLE3 delays female flower anthesis in cucumber Acta Societatis Botanicorum Poloniae cscle3 corolla opening cucumis sativus gene function extra-long ovary |
| title | CsCLE3 delays female flower anthesis in
cucumber |
| title_full | CsCLE3 delays female flower anthesis in
cucumber |
| title_fullStr | CsCLE3 delays female flower anthesis in
cucumber |
| title_full_unstemmed | CsCLE3 delays female flower anthesis in
cucumber |
| title_short | CsCLE3 delays female flower anthesis in
cucumber |
| title_sort | cscle3 delays female flower anthesis in cucumber |
| topic | cscle3 corolla opening cucumis sativus gene function extra-long ovary |
| url | https://www.journalssystem.com/asbp/-CsCLE3-delays-female-flower-anthesis-in-ncucumber,162334,0,2.html |
| work_keys_str_mv | AT mingyuelei cscle3delaysfemalefloweranthesisincucumber AT xiaofeisong cscle3delaysfemalefloweranthesisincucumber AT jinshuangzheng cscle3delaysfemalefloweranthesisincucumber AT chengzhensun cscle3delaysfemalefloweranthesisincucumber AT liyingyan cscle3delaysfemalefloweranthesisincucumber |