Erwinia carotovora subsp. carotovora and Erwinia-Derived Elicitors HrpN and PehA Trigger Distinct but Interacting Defense Responses and Cell Death in Arabidopsis
We have used an hrp-positive strain of the soft rot pathogen Erwinia carotovora subsp. carotovora to elucidate plant responses to this bacterial necrotroph. Purified virulence determinants, harpin (HrpN) and polygalacturonase (PehA), were used as tools to facilitate this analysis. We show that HrpN...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2003-03-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.3.179 |
| _version_ | 1819085395001868288 |
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| author | Tarja Kariola Tiina A. Palomäki Günter Brader E. Tapio Palva |
| author_facet | Tarja Kariola Tiina A. Palomäki Günter Brader E. Tapio Palva |
| author_sort | Tarja Kariola |
| collection | DOAJ |
| description | We have used an hrp-positive strain of the soft rot pathogen Erwinia carotovora subsp. carotovora to elucidate plant responses to this bacterial necrotroph. Purified virulence determinants, harpin (HrpN) and polygalacturonase (PehA), were used as tools to facilitate this analysis. We show that HrpN elicits lesion formation in Arabidopsis and tobacco and triggers systemic resistance in Arabidopsis. Establishment of resistance is accompanied by the expression of salicylic acid (SA)-dependent, but also jasmonate/ethylene (JA/ET)-dependent, marker genes PR1 and PDF1.2, respectively, suggesting that both SA-dependent and JA/ET-dependent defense pathways are activated. Use of pathway-specific mutants and transgenic NahG plants show that both pathways are required for the induction of resistance. Arabidopsis plants treated simultaneously with both elictors PehA, known to trigger only JA/ET-dependent defense signaling, and HrpN react with accelerated and enhanced induction of the marker genes PR1 and PDF1.2 both locally and systemically. This mutual amplification of defense gene expression involves both SA-dependent and JA/ET-dependent defense signaling. The two elicitors produced by E. carotovora subsp. carotovora also cooperate in triggering increased production of superoxide and lesion formation. |
| first_indexed | 2024-12-21T21:03:40Z |
| format | Article |
| id | doaj.art-fc47efd71edf46279318fbf60ee85593 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-21T21:03:40Z |
| publishDate | 2003-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-fc47efd71edf46279318fbf60ee855932022-12-21T18:50:23ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062003-03-0116317918710.1094/MPMI.2003.16.3.179Erwinia carotovora subsp. carotovora and Erwinia-Derived Elicitors HrpN and PehA Trigger Distinct but Interacting Defense Responses and Cell Death in ArabidopsisTarja KariolaTiina A. PalomäkiGünter BraderE. Tapio PalvaWe have used an hrp-positive strain of the soft rot pathogen Erwinia carotovora subsp. carotovora to elucidate plant responses to this bacterial necrotroph. Purified virulence determinants, harpin (HrpN) and polygalacturonase (PehA), were used as tools to facilitate this analysis. We show that HrpN elicits lesion formation in Arabidopsis and tobacco and triggers systemic resistance in Arabidopsis. Establishment of resistance is accompanied by the expression of salicylic acid (SA)-dependent, but also jasmonate/ethylene (JA/ET)-dependent, marker genes PR1 and PDF1.2, respectively, suggesting that both SA-dependent and JA/ET-dependent defense pathways are activated. Use of pathway-specific mutants and transgenic NahG plants show that both pathways are required for the induction of resistance. Arabidopsis plants treated simultaneously with both elictors PehA, known to trigger only JA/ET-dependent defense signaling, and HrpN react with accelerated and enhanced induction of the marker genes PR1 and PDF1.2 both locally and systemically. This mutual amplification of defense gene expression involves both SA-dependent and JA/ET-dependent defense signaling. The two elicitors produced by E. carotovora subsp. carotovora also cooperate in triggering increased production of superoxide and lesion formation.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.3.179induced resistanceprogrammed cell deathsignal pathway |
| spellingShingle | Tarja Kariola Tiina A. Palomäki Günter Brader E. Tapio Palva Erwinia carotovora subsp. carotovora and Erwinia-Derived Elicitors HrpN and PehA Trigger Distinct but Interacting Defense Responses and Cell Death in Arabidopsis Molecular Plant-Microbe Interactions induced resistance programmed cell death signal pathway |
| title | Erwinia carotovora subsp. carotovora and Erwinia-Derived Elicitors HrpN and PehA Trigger Distinct but Interacting Defense Responses and Cell Death in Arabidopsis |
| title_full | Erwinia carotovora subsp. carotovora and Erwinia-Derived Elicitors HrpN and PehA Trigger Distinct but Interacting Defense Responses and Cell Death in Arabidopsis |
| title_fullStr | Erwinia carotovora subsp. carotovora and Erwinia-Derived Elicitors HrpN and PehA Trigger Distinct but Interacting Defense Responses and Cell Death in Arabidopsis |
| title_full_unstemmed | Erwinia carotovora subsp. carotovora and Erwinia-Derived Elicitors HrpN and PehA Trigger Distinct but Interacting Defense Responses and Cell Death in Arabidopsis |
| title_short | Erwinia carotovora subsp. carotovora and Erwinia-Derived Elicitors HrpN and PehA Trigger Distinct but Interacting Defense Responses and Cell Death in Arabidopsis |
| title_sort | erwinia carotovora subsp carotovora and erwinia derived elicitors hrpn and peha trigger distinct but interacting defense responses and cell death in arabidopsis |
| topic | induced resistance programmed cell death signal pathway |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2003.16.3.179 |
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