Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphism
Background and Aim: Milk adulteration is pivotal because it leads to worse effects in public health as human adverse reactions with clinical signs ranged from gastrointestinal signs to anaphylactic shock. This study was carried out to estimate the prevalence of adulteration in buffalo's milk so...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2018-06-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.11/June-2018/15.pdf |
| _version_ | 1818590638430486528 |
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| author | Rania M. Ewida Doaa Safwat M. Abd El-Magiud |
| author_facet | Rania M. Ewida Doaa Safwat M. Abd El-Magiud |
| author_sort | Rania M. Ewida |
| collection | DOAJ |
| description | Background and Aim: Milk adulteration is pivotal because it leads to worse effects in public health as human adverse reactions with clinical signs ranged from gastrointestinal signs to anaphylactic shock. This study was carried out to estimate the prevalence of adulteration in buffalo's milk sold in Assiut City, Egypt.
Materials and Methods: A total of 50 raw buffalo's milk samples were collected and examined for adulteration by addition of cow's milk. The examination carried out by applying polymerase chain reaction-restriction fragment length polymorphism technique using cytochrome b (cyt b) gene primers and Hinf I enzymes. The size of target gene was 360 bp in both animal species and amplicon can be digested using Hinf I enzyme, this restriction enzyme divided the essential band to clear three bands at 360, 210, and 150 bp in cows' milk, while, the enzyme could not be cleaved the amplicon in buffalo's samples.
Results: The obtained results cleared that the incidence of adulteration of buffalo's milk very high percentage reaches 90%.
Conclusion: It could be concluded that the raw buffalo's milk sold in Assiut City subject to fraudulent practice and thus can lead to public health hazards. |
| first_indexed | 2024-12-16T09:59:43Z |
| format | Article |
| id | doaj.art-fc4a6344cd514c268ce2fe1d075642de |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-16T09:59:43Z |
| publishDate | 2018-06-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-fc4a6344cd514c268ce2fe1d075642de2022-12-21T22:35:49ZengVeterinary WorldVeterinary World0972-89882231-09162018-06-0111683083310.14202/vetworld.2018.830-833Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphismRania M. Ewida0Doaa Safwat M. Abd El-Magiud1Department of Food Hygiene (Milk Hygiene), Faculty of Veterinary Medicine, New Valley Branch, Assiut University, Assiut 71526, Egypt.Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, New Valley Branch, Assiut University, Assiut 71526, Egypt.Background and Aim: Milk adulteration is pivotal because it leads to worse effects in public health as human adverse reactions with clinical signs ranged from gastrointestinal signs to anaphylactic shock. This study was carried out to estimate the prevalence of adulteration in buffalo's milk sold in Assiut City, Egypt. Materials and Methods: A total of 50 raw buffalo's milk samples were collected and examined for adulteration by addition of cow's milk. The examination carried out by applying polymerase chain reaction-restriction fragment length polymorphism technique using cytochrome b (cyt b) gene primers and Hinf I enzymes. The size of target gene was 360 bp in both animal species and amplicon can be digested using Hinf I enzyme, this restriction enzyme divided the essential band to clear three bands at 360, 210, and 150 bp in cows' milk, while, the enzyme could not be cleaved the amplicon in buffalo's samples. Results: The obtained results cleared that the incidence of adulteration of buffalo's milk very high percentage reaches 90%. Conclusion: It could be concluded that the raw buffalo's milk sold in Assiut City subject to fraudulent practice and thus can lead to public health hazards.http://www.veterinaryworld.org/Vol.11/June-2018/15.pdfbuffalo's milkcyt b geneHinf I enzymemedicolegalmilk adulterationpolymerase chain reaction-restriction fragment length polymorphism |
| spellingShingle | Rania M. Ewida Doaa Safwat M. Abd El-Magiud Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphism Veterinary World buffalo's milk cyt b gene Hinf I enzyme medicolegal milk adulteration polymerase chain reaction-restriction fragment length polymorphism |
| title | Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphism |
| title_full | Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphism |
| title_fullStr | Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphism |
| title_full_unstemmed | Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphism |
| title_short | Species adulteration in raw milk samples using polymerase chain reaction-restriction fragment length polymorphism |
| title_sort | species adulteration in raw milk samples using polymerase chain reaction restriction fragment length polymorphism |
| topic | buffalo's milk cyt b gene Hinf I enzyme medicolegal milk adulteration polymerase chain reaction-restriction fragment length polymorphism |
| url | http://www.veterinaryworld.org/Vol.11/June-2018/15.pdf |
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