Long noncoding RNAs and circular RNAs as potential diagnostic biomarkers of inflammatory bowel diseases: a systematic review and meta-analysis
IntroductionInflammatory bowel disease (IBD) poses a growing global burden, necessitating the discovery of reliable biomarkers for early diagnosis. The clinical significance of dysregulated expression of long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) in diagnosing IBD has not been well e...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1362437/full |
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| author | Melaku Ashagrie Belete Selamyhun Tadesse Mihret Tilahun Alemu Gedefie Agumas Shibabaw Zewudu Mulatie Muluken Amare Wudu Saba Gebremichael Habtu Debash Mihreteab Alebachew Ermiyas Alemayehu |
| author_facet | Melaku Ashagrie Belete Selamyhun Tadesse Mihret Tilahun Alemu Gedefie Agumas Shibabaw Zewudu Mulatie Muluken Amare Wudu Saba Gebremichael Habtu Debash Mihreteab Alebachew Ermiyas Alemayehu |
| author_sort | Melaku Ashagrie Belete |
| collection | DOAJ |
| description | IntroductionInflammatory bowel disease (IBD) poses a growing global burden, necessitating the discovery of reliable biomarkers for early diagnosis. The clinical significance of dysregulated expression of long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) in diagnosing IBD has not been well established. Thus, our study aimed to investigate the diagnostic value of lncRNAs and circRNAs for IBD based on currently available studies.MethodsA comprehensive search was carried out in diverse electronic databases, such as PubMed, Embase, Scopus, Science Direct and Wiley Online Library to retrieve articles published until October 30, 2023. Stata 17.0 software was employed to determine pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic ratio (DOR), and area under the curve (AUC). Heterogeneity, subgroup analysis, and meta-regression were explored, and publication bias was assessed using Deeks’ funnel plot. Fagan’s nomogram and likelihood ratio scattergram were employed to evaluate the clinical validity.ResultA total of 11 articles encompassing 21 studies which involved 1239 IBD patients and 985 healthy controls were investigated. The findings revealed lncRNAs exhibit high level of pooled sensitivity 0.94 (95% CI: 0.87-0.97) and specificity 0.99 (95% CI: 0.89-1.00), along with PLR, NLR, DOR, and AUC values of 64.25 (95% CI: 7.39-558.66), 0.06 (95% CI: 0.03-0.13), 1055.25 (95% CI: 70.61-15770.77), and 0.99 (95% CI: 0.97-0.99), respectively. Conversely, CircRNAs showed moderate accuracy in IBD diagnosis, with sensitivity of 0.68 (95% CI: 0.61-0.73), specificity of 0.73 (95% CI: 0.65-0.79), PLR of 2.47 (95% CI: 1.94-3.16), NLR of 0.45 (95% CI: 0.38-0.53), DOR of 5.54 (95% CI: 3.88-7.93), and AUC value of 0.75 (95% CI: 0.71-0.79). Moreover, findings from subgroup analysis depicted heightened diagnostic efficacy when employing lncRNA H19 and a large sample size (≥100), with notable efficacy in diagnosing both ulcerative colitis (UC) and Crohn’s disease (CD).ConclusionLncRNAs exhibit high diagnostic accuracy in distinguishing patients with IBD from healthy controls signifying their possible use as potential biomarkers, while circRNAs showed moderate diagnostic accuracy. Nevertheless, to validate our findings and confirm the clinical utility of lncRNAs and circRNAs in IBD diagnosis, a large pool of prospective and multi-center studies should be undertaken.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO, identifier CRD42023491840. |
| first_indexed | 2024-04-25T01:42:44Z |
| format | Article |
| id | doaj.art-fc4aedc2e59143bba84416ea23d9668f |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-25T01:42:44Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-fc4aedc2e59143bba84416ea23d9668f2024-03-08T04:44:31ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-03-011510.3389/fimmu.2024.13624371362437Long noncoding RNAs and circular RNAs as potential diagnostic biomarkers of inflammatory bowel diseases: a systematic review and meta-analysisMelaku Ashagrie Belete0Selamyhun Tadesse1Mihret Tilahun2Alemu Gedefie3Agumas Shibabaw4Zewudu Mulatie5Muluken Amare Wudu6Saba Gebremichael7Habtu Debash8Mihreteab Alebachew9Ermiyas Alemayehu10Department of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaDepartment of Medical Laboratory Science, College of Health Sciences, Woldia University, Woldia, EthiopiaDepartment of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaDepartment of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaDepartment of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaDepartment of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaDepartment of Pediatric and Child Health Nursing, School of Nursing and Midwifery, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaDepartment of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaDepartment of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaDepartment of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaDepartment of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaIntroductionInflammatory bowel disease (IBD) poses a growing global burden, necessitating the discovery of reliable biomarkers for early diagnosis. The clinical significance of dysregulated expression of long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) in diagnosing IBD has not been well established. Thus, our study aimed to investigate the diagnostic value of lncRNAs and circRNAs for IBD based on currently available studies.MethodsA comprehensive search was carried out in diverse electronic databases, such as PubMed, Embase, Scopus, Science Direct and Wiley Online Library to retrieve articles published until October 30, 2023. Stata 17.0 software was employed to determine pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic ratio (DOR), and area under the curve (AUC). Heterogeneity, subgroup analysis, and meta-regression were explored, and publication bias was assessed using Deeks’ funnel plot. Fagan’s nomogram and likelihood ratio scattergram were employed to evaluate the clinical validity.ResultA total of 11 articles encompassing 21 studies which involved 1239 IBD patients and 985 healthy controls were investigated. The findings revealed lncRNAs exhibit high level of pooled sensitivity 0.94 (95% CI: 0.87-0.97) and specificity 0.99 (95% CI: 0.89-1.00), along with PLR, NLR, DOR, and AUC values of 64.25 (95% CI: 7.39-558.66), 0.06 (95% CI: 0.03-0.13), 1055.25 (95% CI: 70.61-15770.77), and 0.99 (95% CI: 0.97-0.99), respectively. Conversely, CircRNAs showed moderate accuracy in IBD diagnosis, with sensitivity of 0.68 (95% CI: 0.61-0.73), specificity of 0.73 (95% CI: 0.65-0.79), PLR of 2.47 (95% CI: 1.94-3.16), NLR of 0.45 (95% CI: 0.38-0.53), DOR of 5.54 (95% CI: 3.88-7.93), and AUC value of 0.75 (95% CI: 0.71-0.79). Moreover, findings from subgroup analysis depicted heightened diagnostic efficacy when employing lncRNA H19 and a large sample size (≥100), with notable efficacy in diagnosing both ulcerative colitis (UC) and Crohn’s disease (CD).ConclusionLncRNAs exhibit high diagnostic accuracy in distinguishing patients with IBD from healthy controls signifying their possible use as potential biomarkers, while circRNAs showed moderate diagnostic accuracy. Nevertheless, to validate our findings and confirm the clinical utility of lncRNAs and circRNAs in IBD diagnosis, a large pool of prospective and multi-center studies should be undertaken.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO, identifier CRD42023491840.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1362437/fulllncRNAscircRNAsdiagnostic biomarkersinflammatory bowel diseaseulcerative colitisCrohn’s disease |
| spellingShingle | Melaku Ashagrie Belete Selamyhun Tadesse Mihret Tilahun Alemu Gedefie Agumas Shibabaw Zewudu Mulatie Muluken Amare Wudu Saba Gebremichael Habtu Debash Mihreteab Alebachew Ermiyas Alemayehu Long noncoding RNAs and circular RNAs as potential diagnostic biomarkers of inflammatory bowel diseases: a systematic review and meta-analysis Frontiers in Immunology lncRNAs circRNAs diagnostic biomarkers inflammatory bowel disease ulcerative colitis Crohn’s disease |
| title | Long noncoding RNAs and circular RNAs as potential diagnostic biomarkers of inflammatory bowel diseases: a systematic review and meta-analysis |
| title_full | Long noncoding RNAs and circular RNAs as potential diagnostic biomarkers of inflammatory bowel diseases: a systematic review and meta-analysis |
| title_fullStr | Long noncoding RNAs and circular RNAs as potential diagnostic biomarkers of inflammatory bowel diseases: a systematic review and meta-analysis |
| title_full_unstemmed | Long noncoding RNAs and circular RNAs as potential diagnostic biomarkers of inflammatory bowel diseases: a systematic review and meta-analysis |
| title_short | Long noncoding RNAs and circular RNAs as potential diagnostic biomarkers of inflammatory bowel diseases: a systematic review and meta-analysis |
| title_sort | long noncoding rnas and circular rnas as potential diagnostic biomarkers of inflammatory bowel diseases a systematic review and meta analysis |
| topic | lncRNAs circRNAs diagnostic biomarkers inflammatory bowel disease ulcerative colitis Crohn’s disease |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1362437/full |
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