TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella
Cilia/flagella are assembled and maintained by the process of intraflagellar transport (IFT), a highly conserved mechanism involving more than 20 IFT proteins. However, the functions of individual IFT proteins are mostly unclear. To help address this issue, we focused on a putative IFT protein TTC26...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2014-03-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/01566 |
| _version_ | 1818018543569993728 |
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| author | Hiroaki Ishikawa Takahiro Ide Toshiki Yagi Xue Jiang Masafumi Hirono Hiroyuki Sasaki Haruaki Yanagisawa Kimberly A Wemmer Didier YR Stainier Hongmin Qin Ritsu Kamiya Wallace F Marshall |
| author_facet | Hiroaki Ishikawa Takahiro Ide Toshiki Yagi Xue Jiang Masafumi Hirono Hiroyuki Sasaki Haruaki Yanagisawa Kimberly A Wemmer Didier YR Stainier Hongmin Qin Ritsu Kamiya Wallace F Marshall |
| author_sort | Hiroaki Ishikawa |
| collection | DOAJ |
| description | Cilia/flagella are assembled and maintained by the process of intraflagellar transport (IFT), a highly conserved mechanism involving more than 20 IFT proteins. However, the functions of individual IFT proteins are mostly unclear. To help address this issue, we focused on a putative IFT protein TTC26/DYF13. Using live imaging and biochemical approaches we show that TTC26/DYF13 is an IFT complex B protein in mammalian cells and Chlamydomonas reinhardtii. Knockdown of TTC26/DYF13 in zebrafish embryos or mutation of TTC26/DYF13 in C. reinhardtii, produced short cilia with abnormal motility. Surprisingly, IFT particle assembly and speed were normal in dyf13 mutant flagella, unlike in other IFT complex B mutants. Proteomic and biochemical analyses indicated a particular set of proteins involved in motility was specifically depleted in the dyf13 mutant. These results support the concept that different IFT proteins are responsible for different cargo subsets, providing a possible explanation for the complexity of the IFT machinery. |
| first_indexed | 2024-04-14T07:41:53Z |
| format | Article |
| id | doaj.art-fc512ec5234640318b6b428f8480ae5e |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:41:53Z |
| publishDate | 2014-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-fc512ec5234640318b6b428f8480ae5e2022-12-22T02:05:29ZengeLife Sciences Publications LtdeLife2050-084X2014-03-01310.7554/eLife.01566TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagellaHiroaki Ishikawa0Takahiro Ide1Toshiki Yagi2Xue Jiang3Masafumi Hirono4Hiroyuki Sasaki5Haruaki Yanagisawa6Kimberly A Wemmer7Didier YR Stainier8Hongmin Qin9Ritsu Kamiya10Wallace F Marshall11Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United StatesDepartment of Biological Science, Graduate School of Science, University of Tokyo, Tokyo, JapanDepartment of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Tokyo, JapanDepartment of Biology, Texas A&M University, College Station, United StatesDepartment of Biological Science, Graduate School of Science, University of Tokyo, Tokyo, JapanDivision of Fine Morphology, Core Research Facilities, The Jikei University School of Medicine, Tokyo, Japan; The Center for Advanced Medical Engineering and Informatics, Osaka University, Osaka, JapanDepartment of Biological Science, Graduate School of Science, University of Tokyo, Tokyo, JapanDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United StatesDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United States; Department of Developmental Genetics, Max Planck Institute for Heart and Lung Research, Bad Nauheim, GermanyDepartment of Biology, Texas A&M University, College Station, United StatesDepartment of Biological Science, Graduate School of Science, University of Tokyo, Tokyo, Japan; Department of Life Science, Faculty of Science, Gakushuin University, Tokyo, JapanDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United StatesCilia/flagella are assembled and maintained by the process of intraflagellar transport (IFT), a highly conserved mechanism involving more than 20 IFT proteins. However, the functions of individual IFT proteins are mostly unclear. To help address this issue, we focused on a putative IFT protein TTC26/DYF13. Using live imaging and biochemical approaches we show that TTC26/DYF13 is an IFT complex B protein in mammalian cells and Chlamydomonas reinhardtii. Knockdown of TTC26/DYF13 in zebrafish embryos or mutation of TTC26/DYF13 in C. reinhardtii, produced short cilia with abnormal motility. Surprisingly, IFT particle assembly and speed were normal in dyf13 mutant flagella, unlike in other IFT complex B mutants. Proteomic and biochemical analyses indicated a particular set of proteins involved in motility was specifically depleted in the dyf13 mutant. These results support the concept that different IFT proteins are responsible for different cargo subsets, providing a possible explanation for the complexity of the IFT machinery.https://elifesciences.org/articles/01566Chlamydomonasflagelladyneinaxoneme |
| spellingShingle | Hiroaki Ishikawa Takahiro Ide Toshiki Yagi Xue Jiang Masafumi Hirono Hiroyuki Sasaki Haruaki Yanagisawa Kimberly A Wemmer Didier YR Stainier Hongmin Qin Ritsu Kamiya Wallace F Marshall TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella eLife Chlamydomonas flagella dynein axoneme |
| title | TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella |
| title_full | TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella |
| title_fullStr | TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella |
| title_full_unstemmed | TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella |
| title_short | TTC26/DYF13 is an intraflagellar transport protein required for transport of motility-related proteins into flagella |
| title_sort | ttc26 dyf13 is an intraflagellar transport protein required for transport of motility related proteins into flagella |
| topic | Chlamydomonas flagella dynein axoneme |
| url | https://elifesciences.org/articles/01566 |
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