Evaluation of the Second Fraction of Excreted /Secreted Antigens from Toxoplasma gondii by Ion Exchange Chromatography forSerodiagnosis of Toxoplasmosis in Rat.
AbstractBackground and Purpose: According to the previous studies, Toxoplasma gondii excreted / secreted antigens (E/SA) appear to be suitable marker for serodiagnosis of toxoplasmosis. Most of the previous studies used whole E/SA. The present study, was carried out to evaluate the ELISA method usin...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2008-01-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://www.mazums.ac.ir/index.php?digital_library&do=downloadPdf&sid=628 |
| _version_ | 1811247607869079552 |
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| author | SH. Abdollahi M. Mahmoodi |
| author_facet | SH. Abdollahi M. Mahmoodi |
| author_sort | SH. Abdollahi |
| collection | DOAJ |
| description | AbstractBackground and Purpose: According to the previous studies, Toxoplasma gondii excreted / secreted antigens (E/SA) appear to be suitable marker for serodiagnosis of toxoplasmosis. Most of the previous studies used whole E/SA. The present study, was carried out to evaluate the ELISA method using E/SA components from Toxoplasma gondii for the diagnosis of toxoplasmosis.Materials and Methods: Components obtained by incubation of tachiziotes at RPMI-1640 culture medium were purified by Ion exchange chromatogeraphy and fractions were analyzed by native-PAGE electrophoresis. Forty noninfected rats were injected as IP with 4×106 Toxoplasma tachyzoites and their serum samples were collected at 8, 15, 22 and 60 days after infection then were tested by dye-test. Based on these results the sera of 15 and 60 days were selected and tested by ELISA using E/SA.Results: Second fraction of chromatography was selected as antigens. The cut-off point of ELISA with 99% confidence was found to be 0.41. Optical density of all sera samples of 15 and 3 of 60 days after infection and 2 negative sera were over the test cut-off. Therefore sensitivity and specificity of the method were determined to be 100% and 91% respectively.Conclusion: These results indicated that the second fraction of Ion exchange chromatography of Toxoplasma E/SA under these conditions may be useful tool for the serediagnosis and differentiation of acute and chronic phases of toxoplasmosis. J Mazand Univ Med Sci 2008; 18(65):42-51 (Persian) |
| first_indexed | 2024-04-12T15:12:37Z |
| format | Article |
| id | doaj.art-fc56981a88f145d3a2a5278caa5ed571 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-12T15:12:37Z |
| publishDate | 2008-01-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-fc56981a88f145d3a2a5278caa5ed5712022-12-22T03:27:43ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792008-01-0118654251Evaluation of the Second Fraction of Excreted /Secreted Antigens from Toxoplasma gondii by Ion Exchange Chromatography forSerodiagnosis of Toxoplasmosis in Rat.SH. AbdollahiM. MahmoodiAbstractBackground and Purpose: According to the previous studies, Toxoplasma gondii excreted / secreted antigens (E/SA) appear to be suitable marker for serodiagnosis of toxoplasmosis. Most of the previous studies used whole E/SA. The present study, was carried out to evaluate the ELISA method using E/SA components from Toxoplasma gondii for the diagnosis of toxoplasmosis.Materials and Methods: Components obtained by incubation of tachiziotes at RPMI-1640 culture medium were purified by Ion exchange chromatogeraphy and fractions were analyzed by native-PAGE electrophoresis. Forty noninfected rats were injected as IP with 4×106 Toxoplasma tachyzoites and their serum samples were collected at 8, 15, 22 and 60 days after infection then were tested by dye-test. Based on these results the sera of 15 and 60 days were selected and tested by ELISA using E/SA.Results: Second fraction of chromatography was selected as antigens. The cut-off point of ELISA with 99% confidence was found to be 0.41. Optical density of all sera samples of 15 and 3 of 60 days after infection and 2 negative sera were over the test cut-off. Therefore sensitivity and specificity of the method were determined to be 100% and 91% respectively.Conclusion: These results indicated that the second fraction of Ion exchange chromatography of Toxoplasma E/SA under these conditions may be useful tool for the serediagnosis and differentiation of acute and chronic phases of toxoplasmosis. J Mazand Univ Med Sci 2008; 18(65):42-51 (Persian)http://www.mazums.ac.ir/index.php?digital_library&do=downloadPdf&sid=628TxoplasmosisExcreted / Secreted AntigensELISAToxoplasma gondii |
| spellingShingle | SH. Abdollahi M. Mahmoodi Evaluation of the Second Fraction of Excreted /Secreted Antigens from Toxoplasma gondii by Ion Exchange Chromatography forSerodiagnosis of Toxoplasmosis in Rat. Journal of Mazandaran University of Medical Sciences Txoplasmosis Excreted / Secreted Antigens ELISA Toxoplasma gondii |
| title | Evaluation of the Second Fraction of Excreted /Secreted Antigens from Toxoplasma gondii by Ion Exchange Chromatography forSerodiagnosis of Toxoplasmosis in Rat. |
| title_full | Evaluation of the Second Fraction of Excreted /Secreted Antigens from Toxoplasma gondii by Ion Exchange Chromatography forSerodiagnosis of Toxoplasmosis in Rat. |
| title_fullStr | Evaluation of the Second Fraction of Excreted /Secreted Antigens from Toxoplasma gondii by Ion Exchange Chromatography forSerodiagnosis of Toxoplasmosis in Rat. |
| title_full_unstemmed | Evaluation of the Second Fraction of Excreted /Secreted Antigens from Toxoplasma gondii by Ion Exchange Chromatography forSerodiagnosis of Toxoplasmosis in Rat. |
| title_short | Evaluation of the Second Fraction of Excreted /Secreted Antigens from Toxoplasma gondii by Ion Exchange Chromatography forSerodiagnosis of Toxoplasmosis in Rat. |
| title_sort | evaluation of the second fraction of excreted secreted antigens from toxoplasma gondii by ion exchange chromatography forserodiagnosis of toxoplasmosis in rat |
| topic | Txoplasmosis Excreted / Secreted Antigens ELISA Toxoplasma gondii |
| url | http://www.mazums.ac.ir/index.php?digital_library&do=downloadPdf&sid=628 |
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