Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients
Background: Type 2 diabetes (T2D) is a multifactorial disease with susceptibility of several genes that are related to T2D. Insulin secretion pathway starts with potassium channels in pancreatic beta cells. KCNJ11 gene encodes ATP-sensitive potassium channel subunits. Some studies suggested that KCN...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Advanced Biomedical Research |
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| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=1;epage=1;aulast=Rastegari |
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| author | Ali Rastegari Mohammad Rabbani Hamid Mirmohammad Sadeghi Elham Faghih Imani Akbar Hasanzadeh Fatemeh Moazen |
| author_facet | Ali Rastegari Mohammad Rabbani Hamid Mirmohammad Sadeghi Elham Faghih Imani Akbar Hasanzadeh Fatemeh Moazen |
| author_sort | Ali Rastegari |
| collection | DOAJ |
| description | Background: Type 2 diabetes (T2D) is a multifactorial disease with susceptibility of several genes that are related to T2D. Insulin secretion pathway starts with potassium channels in pancreatic beta cells. KCNJ11 gene encodes ATP-sensitive potassium channel subunits. Some studies suggested that KCNJ11 (E23K) mutation increases the risk of T2D. Therefore, present study was designed to investigate the association between E23K polymorphism of KCNJ11 gene and type 2 diabetes mellitus (T2DM) in the Iranian population.
Materials and Methods: The type of study was case-control and 40 unrelated subjects, including 20 healthy controls and 20 diabetic patients were recruited (diagnosed based on American Diabetes Association criteria). Blood samples were used for isolation of genomic deoxyribonucleic acid (DNA). Having extracted the genomic DNA from human blood leukocytes by means of High Pure PCR Template Preparation Kit, PCR-restriction fragment length polymorphism method was used to detect KCNJ11 E23K gene polymorphism. BanII restriction enzyme was used for digestion. Data were analyzed using Chi-square or Fisher exact test or independent t-test, as appropriate. P < 0.05 was considered.
Results: We found that the carrier homozygous for KK genotype are susceptible to T2D (0.049) and in patients the frequency of K allele was higher than control subjects (0.048).
Conclusion: The present study suggests that KCNJ11 (E23K) gene polymorphism is associated with T2DM. |
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| issn | 2277-9175 2277-9175 |
| language | English |
| last_indexed | 2024-12-21T02:00:56Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-fc5859686da34792a6341be3b73bde4a2022-12-21T19:19:38ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752277-91752015-01-01411110.4103/2277-9175.148256Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patientsAli RastegariMohammad RabbaniHamid Mirmohammad SadeghiElham Faghih ImaniAkbar HasanzadehFatemeh MoazenBackground: Type 2 diabetes (T2D) is a multifactorial disease with susceptibility of several genes that are related to T2D. Insulin secretion pathway starts with potassium channels in pancreatic beta cells. KCNJ11 gene encodes ATP-sensitive potassium channel subunits. Some studies suggested that KCNJ11 (E23K) mutation increases the risk of T2D. Therefore, present study was designed to investigate the association between E23K polymorphism of KCNJ11 gene and type 2 diabetes mellitus (T2DM) in the Iranian population. Materials and Methods: The type of study was case-control and 40 unrelated subjects, including 20 healthy controls and 20 diabetic patients were recruited (diagnosed based on American Diabetes Association criteria). Blood samples were used for isolation of genomic deoxyribonucleic acid (DNA). Having extracted the genomic DNA from human blood leukocytes by means of High Pure PCR Template Preparation Kit, PCR-restriction fragment length polymorphism method was used to detect KCNJ11 E23K gene polymorphism. BanII restriction enzyme was used for digestion. Data were analyzed using Chi-square or Fisher exact test or independent t-test, as appropriate. P < 0.05 was considered. Results: We found that the carrier homozygous for KK genotype are susceptible to T2D (0.049) and in patients the frequency of K allele was higher than control subjects (0.048). Conclusion: The present study suggests that KCNJ11 (E23K) gene polymorphism is associated with T2DM.http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=1;epage=1;aulast=RastegariIranian populationKCNJ11polymorphismpotassium channelrestriction fragment length polymorphismtype 2 diabetes |
| spellingShingle | Ali Rastegari Mohammad Rabbani Hamid Mirmohammad Sadeghi Elham Faghih Imani Akbar Hasanzadeh Fatemeh Moazen Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients Advanced Biomedical Research Iranian population KCNJ11 polymorphism potassium channel restriction fragment length polymorphism type 2 diabetes |
| title | Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients |
| title_full | Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients |
| title_fullStr | Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients |
| title_full_unstemmed | Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients |
| title_short | Association of KCNJ11 (E23K) gene polymorphism with susceptibility to type 2 diabetes in Iranian patients |
| title_sort | association of kcnj11 e23k gene polymorphism with susceptibility to type 2 diabetes in iranian patients |
| topic | Iranian population KCNJ11 polymorphism potassium channel restriction fragment length polymorphism type 2 diabetes |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2015;volume=4;issue=1;spage=1;epage=1;aulast=Rastegari |
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