Biomineralization of Carbonates Induced by <i>Mucilaginibacter gossypii</i> HFF1: Significant Role of Biochemical Parameters
Although the precipitation of carbonate minerals induced by various bacteria is widely studied, the changes in the biochemical parameters, and their significant role in the biomineralization processes, still need further exploration. In this study, <i>Mucilaginibacter gossypii</i> HFF1 w...
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MDPI AG
2022-05-01
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| Series: | Minerals |
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| Online Access: | https://www.mdpi.com/2075-163X/12/5/614 |
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| author | Bin Sun Junbing Jiang Jiali Tao Zuozhen Han |
| author_facet | Bin Sun Junbing Jiang Jiali Tao Zuozhen Han |
| author_sort | Bin Sun |
| collection | DOAJ |
| description | Although the precipitation of carbonate minerals induced by various bacteria is widely studied, the changes in the biochemical parameters, and their significant role in the biomineralization processes, still need further exploration. In this study, <i>Mucilaginibacter gossypii</i> HFF1 was isolated, identified, and used to induce carbonate minerals at various Mg/Ca ratios. The biochemical parameters were determined in order to explore the biomineralization mechanisms, including cell concentration, pH, ammonia, carbonic anhydrase activity, and alkaline phosphatase activity. The characteristics of extracellular minerals and intracellular inclusions were both analyzed. In addition, the amino acid composition of the extracellular polymeric substance was also tested. Results show that the biochemical parameters provide an alkaline environment for precipitation, due to the combined effect of ammonia, carbonic anhydrase, and alkaline phosphatase. Biotic minerals are characterized by preferred orientation, specific shape, and better crystalline and better thermal stability, indicating their biogenesis. Most of the amino acids in the extracellular polymeric substance are negatived charged, and facilitate the binding of magnesium and calcium ions. The particles with weak crystalline structure in the EPS prove that it acts as a nucleation site. Intracellular analyses prove the presence of the intracellular amorphous inclusions. Our results suggest that the changes in the biochemical parameters caused by bacteria are beneficial to biomineralization, and play a necessary role in its process. This offers new insight into understanding the biomineralization mechanism of the bacteria HFF1. |
| first_indexed | 2024-03-10T03:21:40Z |
| format | Article |
| id | doaj.art-fc5959af295444229949180f1a51ef51 |
| institution | Directory Open Access Journal |
| issn | 2075-163X |
| language | English |
| last_indexed | 2024-03-10T03:21:40Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Minerals |
| spelling | doaj.art-fc5959af295444229949180f1a51ef512023-11-23T12:19:27ZengMDPI AGMinerals2075-163X2022-05-0112561410.3390/min12050614Biomineralization of Carbonates Induced by <i>Mucilaginibacter gossypii</i> HFF1: Significant Role of Biochemical ParametersBin Sun0Junbing Jiang1Jiali Tao2Zuozhen Han3The Cultivation Base of Shanxi Key Laboratory of Mining Area Ecological Restoration and Solid Wastes Utilization, Shanxi Institute of Technology, Yangquan 045000, ChinaThe Cultivation Base of Shanxi Key Laboratory of Mining Area Ecological Restoration and Solid Wastes Utilization, Shanxi Institute of Technology, Yangquan 045000, ChinaThe Cultivation Base of Shanxi Key Laboratory of Mining Area Ecological Restoration and Solid Wastes Utilization, Shanxi Institute of Technology, Yangquan 045000, ChinaShandong Provincial Key Laboratory of Depositional Mineralization and Sedimentary Minerals, College of Earth Science and Engineering, Shandong University of Science and Technology, Qingdao 266590, ChinaAlthough the precipitation of carbonate minerals induced by various bacteria is widely studied, the changes in the biochemical parameters, and their significant role in the biomineralization processes, still need further exploration. In this study, <i>Mucilaginibacter gossypii</i> HFF1 was isolated, identified, and used to induce carbonate minerals at various Mg/Ca ratios. The biochemical parameters were determined in order to explore the biomineralization mechanisms, including cell concentration, pH, ammonia, carbonic anhydrase activity, and alkaline phosphatase activity. The characteristics of extracellular minerals and intracellular inclusions were both analyzed. In addition, the amino acid composition of the extracellular polymeric substance was also tested. Results show that the biochemical parameters provide an alkaline environment for precipitation, due to the combined effect of ammonia, carbonic anhydrase, and alkaline phosphatase. Biotic minerals are characterized by preferred orientation, specific shape, and better crystalline and better thermal stability, indicating their biogenesis. Most of the amino acids in the extracellular polymeric substance are negatived charged, and facilitate the binding of magnesium and calcium ions. The particles with weak crystalline structure in the EPS prove that it acts as a nucleation site. Intracellular analyses prove the presence of the intracellular amorphous inclusions. Our results suggest that the changes in the biochemical parameters caused by bacteria are beneficial to biomineralization, and play a necessary role in its process. This offers new insight into understanding the biomineralization mechanism of the bacteria HFF1.https://www.mdpi.com/2075-163X/12/5/614<i>Mucilaginibacter gossypii</i>biomineralizationbiochemical parametersmolecular dynamicscarbonic anhydraseMg/Ca ratio |
| spellingShingle | Bin Sun Junbing Jiang Jiali Tao Zuozhen Han Biomineralization of Carbonates Induced by <i>Mucilaginibacter gossypii</i> HFF1: Significant Role of Biochemical Parameters Minerals <i>Mucilaginibacter gossypii</i> biomineralization biochemical parameters molecular dynamics carbonic anhydrase Mg/Ca ratio |
| title | Biomineralization of Carbonates Induced by <i>Mucilaginibacter gossypii</i> HFF1: Significant Role of Biochemical Parameters |
| title_full | Biomineralization of Carbonates Induced by <i>Mucilaginibacter gossypii</i> HFF1: Significant Role of Biochemical Parameters |
| title_fullStr | Biomineralization of Carbonates Induced by <i>Mucilaginibacter gossypii</i> HFF1: Significant Role of Biochemical Parameters |
| title_full_unstemmed | Biomineralization of Carbonates Induced by <i>Mucilaginibacter gossypii</i> HFF1: Significant Role of Biochemical Parameters |
| title_short | Biomineralization of Carbonates Induced by <i>Mucilaginibacter gossypii</i> HFF1: Significant Role of Biochemical Parameters |
| title_sort | biomineralization of carbonates induced by i mucilaginibacter gossypii i hff1 significant role of biochemical parameters |
| topic | <i>Mucilaginibacter gossypii</i> biomineralization biochemical parameters molecular dynamics carbonic anhydrase Mg/Ca ratio |
| url | https://www.mdpi.com/2075-163X/12/5/614 |
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