The Antigenic Structure Characterization of Oestrus Ovis Larvae
With the aim of proteic components definition from Oestrus ovis larvae, endowed with antigenic properties, able to induce immune responses in vivo and to react in vitro with induced molecular effectors were been performed: electrophoresis in poliacrilamid gel, western blot technique preceded by immu...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/595 |
| _version_ | 1797214997428830208 |
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| author | Daniela Moţ |
| author_facet | Daniela Moţ |
| author_sort | Daniela Moţ |
| collection | DOAJ |
| description | With the aim of proteic components definition from Oestrus ovis larvae, endowed with antigenic properties, able to induce immune responses in vivo and to react in vitro with induced molecular effectors were been performed: electrophoresis in poliacrilamid gel, western blot technique preceded by immunotransfer, immunoassay test. Total soluble larval antigens of O. ovis were been prepared through ultrasonic disintegration, from all three larval stages. Western blot technique allowed and emphasized the specific antigens with a superior sensitivity in comparison with SDS-PAGE electrophoresis. After antigenic characteristics demonstration of investigated larval antigens were been performed the immunoassay test to emphasized the antibodies dozes for O. ovis infestation diagnosis. |
| first_indexed | 2024-04-24T11:23:04Z |
| format | Article |
| id | doaj.art-fc5b172d0a5741828b8075ec29b554ed |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:23:04Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-fc5b172d0a5741828b8075ec29b554ed2024-04-11T04:13:08ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-01451314314595The Antigenic Structure Characterization of Oestrus Ovis LarvaeDaniela Moţ0University of Agricultural Sciences and Veterinary Medicine from Timişoara, Faculty of Animal Sciences and Biotechnologies, 300645-Timişoara, Aradului, 119, RomaniaWith the aim of proteic components definition from Oestrus ovis larvae, endowed with antigenic properties, able to induce immune responses in vivo and to react in vitro with induced molecular effectors were been performed: electrophoresis in poliacrilamid gel, western blot technique preceded by immunotransfer, immunoassay test. Total soluble larval antigens of O. ovis were been prepared through ultrasonic disintegration, from all three larval stages. Western blot technique allowed and emphasized the specific antigens with a superior sensitivity in comparison with SDS-PAGE electrophoresis. After antigenic characteristics demonstration of investigated larval antigens were been performed the immunoassay test to emphasized the antibodies dozes for O. ovis infestation diagnosis.https://spasb.ro/index.php/public_html/article/view/595antigenselectrophoresisimmunotransferoestrus ovis larvaewestern blot |
| spellingShingle | Daniela Moţ The Antigenic Structure Characterization of Oestrus Ovis Larvae Scientific Papers Animal Science and Biotechnologies antigens electrophoresis immunotransfer oestrus ovis larvae western blot |
| title | The Antigenic Structure Characterization of Oestrus Ovis Larvae |
| title_full | The Antigenic Structure Characterization of Oestrus Ovis Larvae |
| title_fullStr | The Antigenic Structure Characterization of Oestrus Ovis Larvae |
| title_full_unstemmed | The Antigenic Structure Characterization of Oestrus Ovis Larvae |
| title_short | The Antigenic Structure Characterization of Oestrus Ovis Larvae |
| title_sort | antigenic structure characterization of oestrus ovis larvae |
| topic | antigens electrophoresis immunotransfer oestrus ovis larvae western blot |
| url | https://spasb.ro/index.php/public_html/article/view/595 |
| work_keys_str_mv | AT danielamot theantigenicstructurecharacterizationofoestrusovislarvae AT danielamot antigenicstructurecharacterizationofoestrusovislarvae |