Proliferation and differentiation potential of human adipose-derived stem cells grown on chitosan hydrogel.
Applied tissue engineering in regenerative medicine warrants our enhanced understanding of the biomaterials and its function. The aim of this study was to evaluate the proliferation and differentiation potential of human adipose-derived stem cells (hADSCs) grown on chitosan hydrogel. The stability o...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4352002?pdf=render |
| _version_ | 1818369097543450624 |
|---|---|
| author | Tanya Debnath Sutapa Ghosh Usha Shalini Potlapuvu Lakshmi Kona Suguna Ratnakar Kamaraju Suprabhat Sarkar Sumanlatha Gaddam Lakshmi Kiran Chelluri |
| author_facet | Tanya Debnath Sutapa Ghosh Usha Shalini Potlapuvu Lakshmi Kona Suguna Ratnakar Kamaraju Suprabhat Sarkar Sumanlatha Gaddam Lakshmi Kiran Chelluri |
| author_sort | Tanya Debnath |
| collection | DOAJ |
| description | Applied tissue engineering in regenerative medicine warrants our enhanced understanding of the biomaterials and its function. The aim of this study was to evaluate the proliferation and differentiation potential of human adipose-derived stem cells (hADSCs) grown on chitosan hydrogel. The stability of this hydrogel is pH-dependent and its swelling property is pivotal in providing a favorable matrix for cell growth. The study utilized an economical method of cross linking the chitosan with 0.5% glutaraldehyde. Following the isolation of hADSCs from omentum tissue, these cells were cultured and characterized on chitosan hydrogel. Subsequent assays that were performed included JC-1 staining for the mitochondrial integrity as a surrogate marker for viability, cell proliferation and growth kinetics by MTT assay, lineage specific differentiation under two-dimensional culture conditions. Confocal imaging, scanning electron microscopy (SEM), and flow cytometry were used to evaluate these assays. The study revealed that chitosan hydrogel promotes cell proliferation coupled with > 90% cell viability. Cytotoxicity assays demonstrated safety profile. Furthermore, glutaraldehyde cross linked chitosan showed < 5% cytotoxicity, thus serving as a scaffold and facilitating the expansion and differentiation of hADSCs across endoderm, ectoderm and mesoderm lineages. Additional functionalities can be added to this hydrogel, particularly those that regulate stem cell fate. |
| first_indexed | 2024-12-13T23:18:26Z |
| format | Article |
| id | doaj.art-fc5c27f76431438eb7f93d4e44f171c4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T23:18:26Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fc5c27f76431438eb7f93d4e44f171c42022-12-21T23:27:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e012080310.1371/journal.pone.0120803Proliferation and differentiation potential of human adipose-derived stem cells grown on chitosan hydrogel.Tanya DebnathSutapa GhoshUsha Shalini PotlapuvuLakshmi KonaSuguna Ratnakar KamarajuSuprabhat SarkarSumanlatha GaddamLakshmi Kiran ChelluriApplied tissue engineering in regenerative medicine warrants our enhanced understanding of the biomaterials and its function. The aim of this study was to evaluate the proliferation and differentiation potential of human adipose-derived stem cells (hADSCs) grown on chitosan hydrogel. The stability of this hydrogel is pH-dependent and its swelling property is pivotal in providing a favorable matrix for cell growth. The study utilized an economical method of cross linking the chitosan with 0.5% glutaraldehyde. Following the isolation of hADSCs from omentum tissue, these cells were cultured and characterized on chitosan hydrogel. Subsequent assays that were performed included JC-1 staining for the mitochondrial integrity as a surrogate marker for viability, cell proliferation and growth kinetics by MTT assay, lineage specific differentiation under two-dimensional culture conditions. Confocal imaging, scanning electron microscopy (SEM), and flow cytometry were used to evaluate these assays. The study revealed that chitosan hydrogel promotes cell proliferation coupled with > 90% cell viability. Cytotoxicity assays demonstrated safety profile. Furthermore, glutaraldehyde cross linked chitosan showed < 5% cytotoxicity, thus serving as a scaffold and facilitating the expansion and differentiation of hADSCs across endoderm, ectoderm and mesoderm lineages. Additional functionalities can be added to this hydrogel, particularly those that regulate stem cell fate.http://europepmc.org/articles/PMC4352002?pdf=render |
| spellingShingle | Tanya Debnath Sutapa Ghosh Usha Shalini Potlapuvu Lakshmi Kona Suguna Ratnakar Kamaraju Suprabhat Sarkar Sumanlatha Gaddam Lakshmi Kiran Chelluri Proliferation and differentiation potential of human adipose-derived stem cells grown on chitosan hydrogel. PLoS ONE |
| title | Proliferation and differentiation potential of human adipose-derived stem cells grown on chitosan hydrogel. |
| title_full | Proliferation and differentiation potential of human adipose-derived stem cells grown on chitosan hydrogel. |
| title_fullStr | Proliferation and differentiation potential of human adipose-derived stem cells grown on chitosan hydrogel. |
| title_full_unstemmed | Proliferation and differentiation potential of human adipose-derived stem cells grown on chitosan hydrogel. |
| title_short | Proliferation and differentiation potential of human adipose-derived stem cells grown on chitosan hydrogel. |
| title_sort | proliferation and differentiation potential of human adipose derived stem cells grown on chitosan hydrogel |
| url | http://europepmc.org/articles/PMC4352002?pdf=render |
| work_keys_str_mv | AT tanyadebnath proliferationanddifferentiationpotentialofhumanadiposederivedstemcellsgrownonchitosanhydrogel AT sutapaghosh proliferationanddifferentiationpotentialofhumanadiposederivedstemcellsgrownonchitosanhydrogel AT ushashalinipotlapuvu proliferationanddifferentiationpotentialofhumanadiposederivedstemcellsgrownonchitosanhydrogel AT lakshmikona proliferationanddifferentiationpotentialofhumanadiposederivedstemcellsgrownonchitosanhydrogel AT sugunaratnakarkamaraju proliferationanddifferentiationpotentialofhumanadiposederivedstemcellsgrownonchitosanhydrogel AT suprabhatsarkar proliferationanddifferentiationpotentialofhumanadiposederivedstemcellsgrownonchitosanhydrogel AT sumanlathagaddam proliferationanddifferentiationpotentialofhumanadiposederivedstemcellsgrownonchitosanhydrogel AT lakshmikiranchelluri proliferationanddifferentiationpotentialofhumanadiposederivedstemcellsgrownonchitosanhydrogel |