High-frequency (50 Hz) electroacupuncture ameliorates cognitive impairment in rats with amyloid beta 1–42-induced Alzheimer's disease
Acupuncture has been shown to ameliorate cognitive impairment of Alzheimer's disease. Acupoints and stimulation frequency influence the therapeutic effect of electroacupuncture. Rat models of Alzheimer's disease were established by injecting amyloid beta 1–42 (Aβ1–42) into the bilateral la...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2018-01-01
|
| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=10;spage=1833;epage=1841;aulast=Yu |
| _version_ | 1811206474523738112 |
|---|---|
| author | Chao-Chao Yu Ying Wang Feng Shen Li-Hong Kong Ya-Wen Wang Hua Zhou Lei Tang |
| author_facet | Chao-Chao Yu Ying Wang Feng Shen Li-Hong Kong Ya-Wen Wang Hua Zhou Lei Tang |
| author_sort | Chao-Chao Yu |
| collection | DOAJ |
| description | Acupuncture has been shown to ameliorate cognitive impairment of Alzheimer's disease. Acupoints and stimulation frequency influence the therapeutic effect of electroacupuncture. Rat models of Alzheimer's disease were established by injecting amyloid beta 1–42 (Aβ1–42) into the bilateral lateral ventricles. Electroacupuncture at 2, 30, and 50 Hz was carried out at Baihui (GV20; 15° obliquely to a depth of 2 mm) and Shenshu (BL23; perpendicularly to 4–6 mm depth), once a day for 20 minutes (each), for 15 days, taking a break every 7 days. The Morris water maze test was conducted to assess the learning and memory. The expression levels of glycogen synthase kinase-3β (GSK-3β), pSer9-GSK-3β, pTyr216-GSK-3β, amyloid precursor protein and Aβ1–40 in the hippocampus were determined by western blot assay. Results demonstrated that electroacupuncture treatment at different frequencies markedly improved learning and memory ability, increased synaptic curvatures, decreased the width of synaptic clefts, thickened postsynaptic densities, and downregulated the expression of GSK-3β, amyloid precursor protein, and Aβ1–40. pSer9-GSK-3β expression markedly decreased, while pTyr216-GSK-3β expression increased. High-frequency (50 Hz) electroacupuncture was more effective than low (2 Hz) or medium-frequency (30 Hz) electroacupuncture. In conclusion, electroacupuncture treatment exerts a protective effect against Aβ1–42-induced learning and memory deficits and synapse-ultrastructure impairment via inhibition of GSK-3β activity. Moreover, high-frequency electroacupuncture was the most effective therapy. |
| first_indexed | 2024-04-12T03:49:12Z |
| format | Article |
| id | doaj.art-fc6032c9026a45e58b5adb2d40afcd17 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-04-12T03:49:12Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-fc6032c9026a45e58b5adb2d40afcd172022-12-22T03:49:03ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742018-01-0113101833184110.4103/1673-5374.238620High-frequency (50 Hz) electroacupuncture ameliorates cognitive impairment in rats with amyloid beta 1–42-induced Alzheimer's diseaseChao-Chao YuYing WangFeng ShenLi-Hong KongYa-Wen WangHua ZhouLei TangAcupuncture has been shown to ameliorate cognitive impairment of Alzheimer's disease. Acupoints and stimulation frequency influence the therapeutic effect of electroacupuncture. Rat models of Alzheimer's disease were established by injecting amyloid beta 1–42 (Aβ1–42) into the bilateral lateral ventricles. Electroacupuncture at 2, 30, and 50 Hz was carried out at Baihui (GV20; 15° obliquely to a depth of 2 mm) and Shenshu (BL23; perpendicularly to 4–6 mm depth), once a day for 20 minutes (each), for 15 days, taking a break every 7 days. The Morris water maze test was conducted to assess the learning and memory. The expression levels of glycogen synthase kinase-3β (GSK-3β), pSer9-GSK-3β, pTyr216-GSK-3β, amyloid precursor protein and Aβ1–40 in the hippocampus were determined by western blot assay. Results demonstrated that electroacupuncture treatment at different frequencies markedly improved learning and memory ability, increased synaptic curvatures, decreased the width of synaptic clefts, thickened postsynaptic densities, and downregulated the expression of GSK-3β, amyloid precursor protein, and Aβ1–40. pSer9-GSK-3β expression markedly decreased, while pTyr216-GSK-3β expression increased. High-frequency (50 Hz) electroacupuncture was more effective than low (2 Hz) or medium-frequency (30 Hz) electroacupuncture. In conclusion, electroacupuncture treatment exerts a protective effect against Aβ1–42-induced learning and memory deficits and synapse-ultrastructure impairment via inhibition of GSK-3β activity. Moreover, high-frequency electroacupuncture was the most effective therapy.http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=10;spage=1833;epage=1841;aulast=Yunerve regeneration; electroacupuncture; different frequencies; Alzheimer′s disease; cognitive impairment; hippocampus; glycogen synthase kinase-3β; synaptic curvatures; width of synaptic cleft; postsynaptic density; Baihui (GV20); Shenshu (BL23); neural regeneration |
| spellingShingle | Chao-Chao Yu Ying Wang Feng Shen Li-Hong Kong Ya-Wen Wang Hua Zhou Lei Tang High-frequency (50 Hz) electroacupuncture ameliorates cognitive impairment in rats with amyloid beta 1–42-induced Alzheimer's disease Neural Regeneration Research nerve regeneration; electroacupuncture; different frequencies; Alzheimer′s disease; cognitive impairment; hippocampus; glycogen synthase kinase-3β; synaptic curvatures; width of synaptic cleft; postsynaptic density; Baihui (GV20); Shenshu (BL23); neural regeneration |
| title | High-frequency (50 Hz) electroacupuncture ameliorates cognitive impairment in rats with amyloid beta 1–42-induced Alzheimer's disease |
| title_full | High-frequency (50 Hz) electroacupuncture ameliorates cognitive impairment in rats with amyloid beta 1–42-induced Alzheimer's disease |
| title_fullStr | High-frequency (50 Hz) electroacupuncture ameliorates cognitive impairment in rats with amyloid beta 1–42-induced Alzheimer's disease |
| title_full_unstemmed | High-frequency (50 Hz) electroacupuncture ameliorates cognitive impairment in rats with amyloid beta 1–42-induced Alzheimer's disease |
| title_short | High-frequency (50 Hz) electroacupuncture ameliorates cognitive impairment in rats with amyloid beta 1–42-induced Alzheimer's disease |
| title_sort | high frequency 50 hz electroacupuncture ameliorates cognitive impairment in rats with amyloid beta 1 42 induced alzheimer s disease |
| topic | nerve regeneration; electroacupuncture; different frequencies; Alzheimer′s disease; cognitive impairment; hippocampus; glycogen synthase kinase-3β; synaptic curvatures; width of synaptic cleft; postsynaptic density; Baihui (GV20); Shenshu (BL23); neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=10;spage=1833;epage=1841;aulast=Yu |
| work_keys_str_mv | AT chaochaoyu highfrequency50hzelectroacupunctureamelioratescognitiveimpairmentinratswithamyloidbeta142inducedalzheimersdisease AT yingwang highfrequency50hzelectroacupunctureamelioratescognitiveimpairmentinratswithamyloidbeta142inducedalzheimersdisease AT fengshen highfrequency50hzelectroacupunctureamelioratescognitiveimpairmentinratswithamyloidbeta142inducedalzheimersdisease AT lihongkong highfrequency50hzelectroacupunctureamelioratescognitiveimpairmentinratswithamyloidbeta142inducedalzheimersdisease AT yawenwang highfrequency50hzelectroacupunctureamelioratescognitiveimpairmentinratswithamyloidbeta142inducedalzheimersdisease AT huazhou highfrequency50hzelectroacupunctureamelioratescognitiveimpairmentinratswithamyloidbeta142inducedalzheimersdisease AT leitang highfrequency50hzelectroacupunctureamelioratescognitiveimpairmentinratswithamyloidbeta142inducedalzheimersdisease |