| Summary: | Essential oils were obtained from different parts of <i>Agastache foeniculum</i> (<i>Lophanthus anisatus</i>) plants by means of extraction: green extraction using hydro-distillation (HD) and bio-solvent distillation, BiAD, discontinuous distillation, and supercritical fluid extraction, in two stages: (1) with CO<sub>2</sub>, and (2) with CO<sub>2</sub> and ethanol co-solvent. The extraction yields were determined. The yield values varied for different parts of the plant, as well as the method of extraction. Thus, they had the values of 0.62 ± 0.020 and 0.92 ± 0.015 g/100 g for the samples from the whole aerial plant, 0.75 ± 0.008 and 1.06 ± 0.005 g/100 g for the samples of leaves, and 1.22 ± 0.011 and 1.60 ± 0.049 g/100 g for the samples of flowers for HD and BiAD, respectively. The yield values for supercritical fluid extraction were of 0.94 ± 0.010 and 0.32 ± 0.007 g/100 g for the samples of whole aerial plant, 0.9 ± 0.010 and 1.14 ± 0.008 g/100 g for the samples of leaves, and 1.94 ± 0.030 and 0.57 ± 0.003 g/100 g for the samples of flowers, in the first and second stages, respectively. The main components of <i>Lophanthus anisatus</i> were identified as: estragon, limonene, eugenol, chavicol, benzaldehyde, and pentanol. The essential oil from <i>Agatache foeniculum</i> has antimicrobial effects against <i>Staphylococcus aureus</i>, the <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>. Acclimatization of <i>Lophantus anisatus</i> in Romania gives it special qualities by concentrating components such as: estragole over 93%, limonene over 8%, especially in flowers; and chavicol over 14%, estragole over 30%, eugenol and derivatives (methoxy eugenol, methyl eugenol, etc.) over 30% and phenyl ether alcohol over 20% in leaves. As a result of the research carried out, it was proven that <i>Lophanthus anisatus</i> can be used as a medicinal plant for many diseases, it can be used as a spice and preservative for various foods, etc.
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