Oligodendrocyte Progenitors Display Enhanced Proliferation and Autophagy after Space Flight
Intracranial hypertension (ICP) and visual impairment intracranial pressure (VIIP) are some of the consequences of long-term space missions. Here we examined the behavior of oligodendrocyte progenitors (OLPs) after space flight using time-lapse microscopy. We show that most OLPs divided more than gr...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/2/201 |
| _version_ | 1797622171009286144 |
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| author | Victoria Tran Nicholas Carpo Carlos Cepeda Araceli Espinosa-Jeffrey |
| author_facet | Victoria Tran Nicholas Carpo Carlos Cepeda Araceli Espinosa-Jeffrey |
| author_sort | Victoria Tran |
| collection | DOAJ |
| description | Intracranial hypertension (ICP) and visual impairment intracranial pressure (VIIP) are some of the consequences of long-term space missions. Here we examined the behavior of oligodendrocyte progenitors (OLPs) after space flight using time-lapse microscopy. We show that most OLPs divided more than ground control (GC) counterparts did. Nonetheless, a subpopulation of OLPs flown to space presented a significant increase in autophagic cell death. Examination of the proteomic profile of the secretome of space flown OLPs (SPC-OLPs) revealed that the stress protein heat shock protein-90 beta “HSP-90β” was the 5<sup>th</sup> most enriched (6.8 times) and the secreted protein acidic and rich in cysteine “SPARC” was the 7<sup>th</sup> most enriched (5.2 times), with respect to ground control cells. SPARC induces endoplasmic reticulum stress, which leads to autophagy. Given the roles and importance of these two proteins in mammalian cells’ metabolism, their upregulation may hold the key to modulating cell proliferation and autophagy, in order to mitigate ICP and VIIP during and after space missions. |
| first_indexed | 2024-03-11T09:06:32Z |
| format | Article |
| id | doaj.art-fc655c71006d47389a7935a7796bd868 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T09:06:32Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-fc655c71006d47389a7935a7796bd8682023-11-16T19:21:48ZengMDPI AGBiomolecules2218-273X2023-01-0113220110.3390/biom13020201Oligodendrocyte Progenitors Display Enhanced Proliferation and Autophagy after Space FlightVictoria Tran0Nicholas Carpo1Carlos Cepeda2Araceli Espinosa-Jeffrey3Department of Psychiatry, Semel Institute for Neuroscience and Human Behavior, The University of California Los Angeles, Los Angeles, CA 90095, USADepartment of Psychiatry, Semel Institute for Neuroscience and Human Behavior, The University of California Los Angeles, Los Angeles, CA 90095, USADepartment of Psychiatry, Semel Institute for Neuroscience and Human Behavior, The University of California Los Angeles, Los Angeles, CA 90095, USADepartment of Psychiatry, Semel Institute for Neuroscience and Human Behavior, The University of California Los Angeles, Los Angeles, CA 90095, USAIntracranial hypertension (ICP) and visual impairment intracranial pressure (VIIP) are some of the consequences of long-term space missions. Here we examined the behavior of oligodendrocyte progenitors (OLPs) after space flight using time-lapse microscopy. We show that most OLPs divided more than ground control (GC) counterparts did. Nonetheless, a subpopulation of OLPs flown to space presented a significant increase in autophagic cell death. Examination of the proteomic profile of the secretome of space flown OLPs (SPC-OLPs) revealed that the stress protein heat shock protein-90 beta “HSP-90β” was the 5<sup>th</sup> most enriched (6.8 times) and the secreted protein acidic and rich in cysteine “SPARC” was the 7<sup>th</sup> most enriched (5.2 times), with respect to ground control cells. SPARC induces endoplasmic reticulum stress, which leads to autophagy. Given the roles and importance of these two proteins in mammalian cells’ metabolism, their upregulation may hold the key to modulating cell proliferation and autophagy, in order to mitigate ICP and VIIP during and after space missions.https://www.mdpi.com/2218-273X/13/2/201space flightoligodendrocyte progenitorsautophagyproliferationvisual impairment intracranial pressureintracranial hypertension |
| spellingShingle | Victoria Tran Nicholas Carpo Carlos Cepeda Araceli Espinosa-Jeffrey Oligodendrocyte Progenitors Display Enhanced Proliferation and Autophagy after Space Flight Biomolecules space flight oligodendrocyte progenitors autophagy proliferation visual impairment intracranial pressure intracranial hypertension |
| title | Oligodendrocyte Progenitors Display Enhanced Proliferation and Autophagy after Space Flight |
| title_full | Oligodendrocyte Progenitors Display Enhanced Proliferation and Autophagy after Space Flight |
| title_fullStr | Oligodendrocyte Progenitors Display Enhanced Proliferation and Autophagy after Space Flight |
| title_full_unstemmed | Oligodendrocyte Progenitors Display Enhanced Proliferation and Autophagy after Space Flight |
| title_short | Oligodendrocyte Progenitors Display Enhanced Proliferation and Autophagy after Space Flight |
| title_sort | oligodendrocyte progenitors display enhanced proliferation and autophagy after space flight |
| topic | space flight oligodendrocyte progenitors autophagy proliferation visual impairment intracranial pressure intracranial hypertension |
| url | https://www.mdpi.com/2218-273X/13/2/201 |
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