Optimizing oxytocin LC-MS/MS sensitivity by choosing the right column

Objective: Sensitivity is often an issue in bioanalytical LC-MS/MS applications. Commonly investigated parameters to improve it include additives to mobile phase, derivatization and sample-preparation. The nature of the column, however, is not frequently evaluated. Design and Methods: The sensitivit...

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Bibliographic Details
Main Author: Mariëtte T. Ackermans
Format: Article
Language:English
Published: Elsevier 2021-11-01
Series:Practical Laboratory Medicine
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352551721000548
Description
Summary:Objective: Sensitivity is often an issue in bioanalytical LC-MS/MS applications. Commonly investigated parameters to improve it include additives to mobile phase, derivatization and sample-preparation. The nature of the column, however, is not frequently evaluated. Design and Methods: The sensitivity is compared for 18 different reversed phase and 2 different HILIC columns using 2 different mobile phase compositions. Sensitivity was evaluated in terms of S/N for 1,5 pg oxytocin on column, using a scouting gradient. Results: The measured signal to noise ranged from 55 to 1473, indicating a substantial difference in sensitivity. The most sensitive columns were the Synergi Hydro RP (reversed phase) and the Atlantis HILIC (HILIC). Conclusions: This study shows that choosing the right column contributes to the sensitivity of the method.
ISSN:2352-5517