Comparative staining of Rhinolophus spp. white blood cells in blood smears
Background and Aim: A drawback of studies on bat blood smears in the field is the lack of time for fixation because blood sampling using a non-lethal method often provides less time for fixation in smear preparations due to the small volume of blood collected. Usually, there is insufficient blood fo...
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| Format: | Article |
| Language: | English |
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Veterinary World
2022-03-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.15/March-2022/28.pdf |
| _version_ | 1811332425473589248 |
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| author | Astghik Ghazaryan Seda Adamyan Tigran Hayrapetyan George Papov Lina Hakobyan Liana Abroyan Nane Bayramyan Sona Hakobyan Arpine Poghosyan Hrag Torossian Zaven Karalyan |
| author_facet | Astghik Ghazaryan Seda Adamyan Tigran Hayrapetyan George Papov Lina Hakobyan Liana Abroyan Nane Bayramyan Sona Hakobyan Arpine Poghosyan Hrag Torossian Zaven Karalyan |
| author_sort | Astghik Ghazaryan |
| collection | DOAJ |
| description | Background and Aim: A drawback of studies on bat blood smears in the field is the lack of time for fixation because blood sampling using a non-lethal method often provides less time for fixation in smear preparations due to the small volume of blood collected. Usually, there is insufficient blood for another smear preparation, so it is necessary to use blood smears as rationally as possible, especially for rare bats. Many stains are used for staining peripheral blood smears, and they have advantages and disadvantages. This study aimed to examine commonly used stains for blood smears to select the best stain for staining peripheral blood smears in bats.
Materials and Methods: In this study, 48 blood smears of Rhinolophus spp. bats were examined using several blood staining methods. Four methods that showed the best results were used in further experiments: Romanowsky-Giemsa, Pappenheim, hematoxylin-eosin, and eosin methylene blue.
Results: Comparative analysis of different methods for staining bat blood smears revealed that the most convenient method for analyzing blood cells is Pappenheim method.
Conclusion: Staining blood smears using Pappenheim method yield the least number of unsuccessful blood smear stains and are quite effective for the morphological analysis of blood cells. |
| first_indexed | 2024-04-13T16:36:47Z |
| format | Article |
| id | doaj.art-fc69f05b48a148f28f14fe8f7682e3fd |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-04-13T16:36:47Z |
| publishDate | 2022-03-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-fc69f05b48a148f28f14fe8f7682e3fd2022-12-22T02:39:24ZengVeterinary WorldVeterinary World0972-89882231-09162022-03-0115375075610.14202/vetworld.2022.750-756Comparative staining of Rhinolophus spp. white blood cells in blood smearsAstghik Ghazaryan0Seda Adamyan1Tigran Hayrapetyan2George Papov3Lina Hakobyan4Liana Abroyan5Nane Bayramyan6Sona Hakobyan7https://orcid.org/0000-0003-3579-0738Arpine Poghosyan8Hrag Torossian9Zaven Karalyan10https://orcid.org/0000-0003-0861-2207Department of Zoology, Faculty of Biology, Yerevan State University, A. Manoogian 1, 0025, Yerevan, Armenia.Department of Zoology, Faculty of Biology, Yerevan State University, A. Manoogian 1, 0025, Yerevan, Armenia.Department of Zoology, Faculty of Biology, Yerevan State University, A. Manoogian 1, 0025, Yerevan, Armenia.Department of Zoology, Faculty of Biology, Yerevan State University, A. Manoogian 1, 0025, Yerevan, Armenia.Laboratory of Cell Biology and Virology, Institute of Molecular Biology NAS RA, Yerevan, Armenia.Laboratory of Cell Biology and Virology, Institute of Molecular Biology NAS RA, Yerevan, Armenia.Laboratory of Cell Biology and Virology, Institute of Molecular Biology NAS RA, Yerevan, Armenia.Laboratory of Cell Biology and Virology, Institute of Molecular Biology NAS RA, Yerevan, Armenia.Laboratory of Cell Biology and Virology, Institute of Molecular Biology NAS RA, Yerevan, Armenia.Department of Medical Biology, Yerevan State Medical University after M. Heratsi, Yerevan, Armenia.Laboratory of Cell Biology and Virology, Institute of Molecular Biology NAS RA, Yerevan, Armenia; Department of Medical Biology, Yerevan State Medical University after M. Heratsi, Yerevan, Armenia.Background and Aim: A drawback of studies on bat blood smears in the field is the lack of time for fixation because blood sampling using a non-lethal method often provides less time for fixation in smear preparations due to the small volume of blood collected. Usually, there is insufficient blood for another smear preparation, so it is necessary to use blood smears as rationally as possible, especially for rare bats. Many stains are used for staining peripheral blood smears, and they have advantages and disadvantages. This study aimed to examine commonly used stains for blood smears to select the best stain for staining peripheral blood smears in bats. Materials and Methods: In this study, 48 blood smears of Rhinolophus spp. bats were examined using several blood staining methods. Four methods that showed the best results were used in further experiments: Romanowsky-Giemsa, Pappenheim, hematoxylin-eosin, and eosin methylene blue. Results: Comparative analysis of different methods for staining bat blood smears revealed that the most convenient method for analyzing blood cells is Pappenheim method. Conclusion: Staining blood smears using Pappenheim method yield the least number of unsuccessful blood smear stains and are quite effective for the morphological analysis of blood cells.http://www.veterinaryworld.org/Vol.15/March-2022/28.pdfblood smearsrhinolophidaestainingwhite blood cells |
| spellingShingle | Astghik Ghazaryan Seda Adamyan Tigran Hayrapetyan George Papov Lina Hakobyan Liana Abroyan Nane Bayramyan Sona Hakobyan Arpine Poghosyan Hrag Torossian Zaven Karalyan Comparative staining of Rhinolophus spp. white blood cells in blood smears Veterinary World blood smears rhinolophidae staining white blood cells |
| title | Comparative staining of Rhinolophus spp. white blood cells in blood smears |
| title_full | Comparative staining of Rhinolophus spp. white blood cells in blood smears |
| title_fullStr | Comparative staining of Rhinolophus spp. white blood cells in blood smears |
| title_full_unstemmed | Comparative staining of Rhinolophus spp. white blood cells in blood smears |
| title_short | Comparative staining of Rhinolophus spp. white blood cells in blood smears |
| title_sort | comparative staining of rhinolophus spp white blood cells in blood smears |
| topic | blood smears rhinolophidae staining white blood cells |
| url | http://www.veterinaryworld.org/Vol.15/March-2022/28.pdf |
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