Extraction, isolation and antioxidant activity of the polysaccharides from embryo of the seeds of Nelumbo nucifera Gaertn(莲子心多糖的提取、分离和抗氧化活性研究)

Extraction, isolation and antioxidant activity of the polysaccharides from embryo of the seeds of Nelumbo nucifera Gaertn(莲子心多糖的提取、分离和抗氧化活性研究)

干燥的莲子心经机械粉碎、乙醇脱脂、沸水提取、乙醇沉淀及中性酶和Sevag法除蛋白得其粗多糖，经DEAE-Cellulose-32阴离子交换和Sephacryl™ S-200凝胶过滤层析得组份ELPS-Ⅰ和混合物Ⅱ，Ⅱ再经过DEAE-Cellulose-32柱层析得组份ELPS-Ⅱ . GC-MS法测定粗多糖、ELPS-Ⅰ和ELPS-Ⅱ的中性糖组成.凝胶过滤色谱(GPC)法测得ELPS-Ⅰ和ELPS-Ⅱ的重均分子量分别为4.97×104 Da和2.96×106Da. β-胡萝卜素漂白实验结果显示：莲子心粗多糖具有显著的抗氧化活性（维生素C作为阳性对照）....

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Bibliographic Details
Main Authors:	YUYuan-zhi(俞远志), WUYa-lin(吴亚林), PANYuan-jiang(潘远江)
Format:	Article
Language:	zho
Published:	Zhejiang University Press 2008-01-01
Series:	Zhejiang Daxue xuebao. Lixue ban
Subjects:	莲子心多糖抗氧化活性
Online Access:	https://doi.org/zjup/1008-9497.2008.35.1.48-51

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