Optimization of Extraction Process and Analysis of Physicochemical Properties of Wild Rice Protein
Wild rice was used as raw material to extract protein by alkali extraction and acid precipitation. Based on the single-factor test, the best extraction process of wild rice protein was obtained by using the index of extraction rate of rice protein and the optimization of the response surface method....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022060125 |
| _version_ | 1797737819641217024 |
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| author | Meng YUAN Chunmei LI Jiamin SU Yu ZHOU Yansong CHEN Haifeng ZHANG |
| author_facet | Meng YUAN Chunmei LI Jiamin SU Yu ZHOU Yansong CHEN Haifeng ZHANG |
| author_sort | Meng YUAN |
| collection | DOAJ |
| description | Wild rice was used as raw material to extract protein by alkali extraction and acid precipitation. Based on the single-factor test, the best extraction process of wild rice protein was obtained by using the index of extraction rate of rice protein and the optimization of the response surface method. The wild rice protein powder was obtained by isoelectric point precipitation and freeze-drying. Compared with the Huai rice protein extracted under the same conditions, wild rice protein's molecular composition, thermal stability, and digestion characteristics were compared and analyzed. The results showed that the effects of different extraction conditions on the extraction of wild rice protein by alkali extraction and acid precipitation were as follows: Ratio of material to liquid>temperature>time, and the optimum extraction conditions were as follows: Extraction temperature 58 ℃, the ratio of material to liquid 1:25 g/mL, extraction time 3.5 h. Under these conditions, the wild rice protein extraction rate was 45.97%, and the isoelectric point (pH) of wild rice protein was 3.5. Compared with Huai rice protein, the denaturation peak temperature of wild rice protein was 108.4 ℃, ΔH=164.4 J/g, the high molecular weight protein content was less, and the digestibility was the same, however, the content of free amino acids was always higher, which was more beneficial to human digestion and absorption. In conclusion, wild rice protein is a high quality source of supplemental protein for human body. |
| first_indexed | 2024-03-12T13:34:57Z |
| format | Article |
| id | doaj.art-fc6fe9d5784d4e74aab51090807a66bf |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:34:57Z |
| publishDate | 2023-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-fc6fe9d5784d4e74aab51090807a66bf2023-08-24T06:01:19ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-06-01441117117810.13386/j.issn1002-0306.20220601252022060125-11Optimization of Extraction Process and Analysis of Physicochemical Properties of Wild Rice ProteinMeng YUAN0Chunmei LI1Jiamin SU2Yu ZHOU3Yansong CHEN4Haifeng ZHANG5School of Food Science and Engineering, Yangzhou University, Yangzhou 225100, ChinaSchool of Food Science and Engineering, Yangzhou University, Yangzhou 225100, ChinaSchool of Food Science and Engineering, Yangzhou University, Yangzhou 225100, ChinaSchool of Food Science and Engineering, Yangzhou University, Yangzhou 225100, ChinaSchool of Food Science and Engineering, Yangzhou University, Yangzhou 225100, ChinaSchool of Food Science and Engineering, Yangzhou University, Yangzhou 225100, ChinaWild rice was used as raw material to extract protein by alkali extraction and acid precipitation. Based on the single-factor test, the best extraction process of wild rice protein was obtained by using the index of extraction rate of rice protein and the optimization of the response surface method. The wild rice protein powder was obtained by isoelectric point precipitation and freeze-drying. Compared with the Huai rice protein extracted under the same conditions, wild rice protein's molecular composition, thermal stability, and digestion characteristics were compared and analyzed. The results showed that the effects of different extraction conditions on the extraction of wild rice protein by alkali extraction and acid precipitation were as follows: Ratio of material to liquid>temperature>time, and the optimum extraction conditions were as follows: Extraction temperature 58 ℃, the ratio of material to liquid 1:25 g/mL, extraction time 3.5 h. Under these conditions, the wild rice protein extraction rate was 45.97%, and the isoelectric point (pH) of wild rice protein was 3.5. Compared with Huai rice protein, the denaturation peak temperature of wild rice protein was 108.4 ℃, ΔH=164.4 J/g, the high molecular weight protein content was less, and the digestibility was the same, however, the content of free amino acids was always higher, which was more beneficial to human digestion and absorption. In conclusion, wild rice protein is a high quality source of supplemental protein for human body.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022060125wild rice proteinextraction processresponse surfacephysicochemical propertiesdigestion |
| spellingShingle | Meng YUAN Chunmei LI Jiamin SU Yu ZHOU Yansong CHEN Haifeng ZHANG Optimization of Extraction Process and Analysis of Physicochemical Properties of Wild Rice Protein Shipin gongye ke-ji wild rice protein extraction process response surface physicochemical properties digestion |
| title | Optimization of Extraction Process and Analysis of Physicochemical Properties of Wild Rice Protein |
| title_full | Optimization of Extraction Process and Analysis of Physicochemical Properties of Wild Rice Protein |
| title_fullStr | Optimization of Extraction Process and Analysis of Physicochemical Properties of Wild Rice Protein |
| title_full_unstemmed | Optimization of Extraction Process and Analysis of Physicochemical Properties of Wild Rice Protein |
| title_short | Optimization of Extraction Process and Analysis of Physicochemical Properties of Wild Rice Protein |
| title_sort | optimization of extraction process and analysis of physicochemical properties of wild rice protein |
| topic | wild rice protein extraction process response surface physicochemical properties digestion |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022060125 |
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