Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis
To elucidate the toxic mechanism of snake venom at the protein level, proteomics technology was applied to investigate the effect of venom on circulation in the mammalian body. Temporal proteomic analysis was performed to profile the dynamic changes in the sera of Sprague–Dawley rats administered wi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2020-10-01
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| Series: | Forensic Sciences Research |
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| Online Access: | http://dx.doi.org/10.1080/20961790.2017.1405565 |
| _version_ | 1797717506684616704 |
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| author | Hui Yan Ping Xiang Jingshuo Zhang Liqi Xie Min Shen |
| author_facet | Hui Yan Ping Xiang Jingshuo Zhang Liqi Xie Min Shen |
| author_sort | Hui Yan |
| collection | DOAJ |
| description | To elucidate the toxic mechanism of snake venom at the protein level, proteomics technology was applied to investigate the effect of venom on circulation in the mammalian body. Temporal proteomic analysis was performed to profile the dynamic changes in the sera of Sprague–Dawley rats administered with Chinese cobra venom or saline. Using 8-plex iTRAQ analysis, 392 and 636 serum proteins were identified to be linearly upregulated or downregulated over time in the low-dose group and high-dose group, respectively. These proteins were mainly associated with the acute phase response pathway, complement system, and liver X receptor (LXR)/retinoid X receptor (RXR) and farnesoid X receptor (FXR)/RXR activation pathways. Compared with the low-dose group, the immune response and integrin pathways were inhibited in the high-dose group, although no obvious effect was observed. With consistently higher or lower expression in the high-dose group compared to the low-dose group throughout the whole process of venom poisoning, two proteins, Kininogen-1 (KNG1) and orosomucoid 1 (ORM1), which are involved in metabolism and immune response, occupied a core position in the pathway network and are considered venom dose-dependent biomarker candidates. |
| first_indexed | 2024-03-12T08:37:20Z |
| format | Article |
| id | doaj.art-fc71a2586b5e42c89cbed71fb0059ef4 |
| institution | Directory Open Access Journal |
| issn | 2096-1790 2471-1411 |
| language | English |
| last_indexed | 2024-03-12T08:37:20Z |
| publishDate | 2020-10-01 |
| publisher | Oxford University Press |
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| series | Forensic Sciences Research |
| spelling | doaj.art-fc71a2586b5e42c89cbed71fb0059ef42023-09-02T17:09:29ZengOxford University PressForensic Sciences Research2096-17902471-14112020-10-015430932110.1080/20961790.2017.14055651405565Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysisHui Yan0Ping Xiang1Jingshuo Zhang2Liqi Xie3Min Shen4Shanghai Key Laboratory of Forensic Science, Shanghai Forensic Platform, Department of Forensic Toxicology, Academy of Forensic ScienceShanghai Key Laboratory of Forensic Science, Shanghai Forensic Platform, Department of Forensic Toxicology, Academy of Forensic ScienceCollege of Pharmaceutical Sciences, Soochow UniverstityInstitutes of Biomedical Sciences, Fudan UniversityShanghai Key Laboratory of Forensic Science, Shanghai Forensic Platform, Department of Forensic Toxicology, Academy of Forensic ScienceTo elucidate the toxic mechanism of snake venom at the protein level, proteomics technology was applied to investigate the effect of venom on circulation in the mammalian body. Temporal proteomic analysis was performed to profile the dynamic changes in the sera of Sprague–Dawley rats administered with Chinese cobra venom or saline. Using 8-plex iTRAQ analysis, 392 and 636 serum proteins were identified to be linearly upregulated or downregulated over time in the low-dose group and high-dose group, respectively. These proteins were mainly associated with the acute phase response pathway, complement system, and liver X receptor (LXR)/retinoid X receptor (RXR) and farnesoid X receptor (FXR)/RXR activation pathways. Compared with the low-dose group, the immune response and integrin pathways were inhibited in the high-dose group, although no obvious effect was observed. With consistently higher or lower expression in the high-dose group compared to the low-dose group throughout the whole process of venom poisoning, two proteins, Kininogen-1 (KNG1) and orosomucoid 1 (ORM1), which are involved in metabolism and immune response, occupied a core position in the pathway network and are considered venom dose-dependent biomarker candidates.http://dx.doi.org/10.1080/20961790.2017.1405565forensic sciencesforensic toxicologyproteomicssnake bitecobraserumrats |
| spellingShingle | Hui Yan Ping Xiang Jingshuo Zhang Liqi Xie Min Shen Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis Forensic Sciences Research forensic sciences forensic toxicology proteomics snake bite cobra serum rats |
| title | Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis |
| title_full | Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis |
| title_fullStr | Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis |
| title_full_unstemmed | Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis |
| title_short | Dynamic changes of serum protein in rats with acute intoxication of Chinese cobra snake venom by proteomic analysis |
| title_sort | dynamic changes of serum protein in rats with acute intoxication of chinese cobra snake venom by proteomic analysis |
| topic | forensic sciences forensic toxicology proteomics snake bite cobra serum rats |
| url | http://dx.doi.org/10.1080/20961790.2017.1405565 |
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