Generation of skeletal muscle cells from pluripotent stem cells: advances and challenges
Human pluripotent stem cells (hPSCs) possess unlimited proliferative potential while maintaining the ability to differentiate into any cell type including skeletal muscle cells (SMCs). hPSCs are amenable to genetic editing and can be derived from patient somatic cells, and thus represent a promising...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-05-01
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| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fcell.2015.00029/full |
| _version_ | 1818901911595646976 |
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| author | Ramzey eAbujarour Bahram eValamehr |
| author_facet | Ramzey eAbujarour Bahram eValamehr |
| author_sort | Ramzey eAbujarour |
| collection | DOAJ |
| description | Human pluripotent stem cells (hPSCs) possess unlimited proliferative potential while maintaining the ability to differentiate into any cell type including skeletal muscle cells (SMCs). hPSCs are amenable to genetic editing and can be derived from patient somatic cells, and thus represent a promising option for cell therapies for the treatment of degenerative diseases such as muscular dystrophies. There are unresolved challenges however associated with the derivation and scale-up of hPSCs and generation of differentiated cells in large quantity and high purity. Reported myogenic differentiation protocols are long, require cell sorting and/or rely on ectopic expression of myogenic master regulators. More recent advances have been made with the application of small molecules to enhance the myogenic differentiation efficiency and the identification of more selective markers for the enrichment of myogenic progenitors with enhanced regenerative potential. Here we review the field of myogenic differentiation and highlight areas requiring further research. |
| first_indexed | 2024-12-19T20:27:17Z |
| format | Article |
| id | doaj.art-fc740313a16143aaacda09c96ea4933f |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-19T20:27:17Z |
| publishDate | 2015-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-fc740313a16143aaacda09c96ea4933f2022-12-21T20:06:48ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2015-05-01310.3389/fcell.2015.00029141688Generation of skeletal muscle cells from pluripotent stem cells: advances and challengesRamzey eAbujarour0Bahram eValamehr1Fate TherapeuticsFate TherapeuticsHuman pluripotent stem cells (hPSCs) possess unlimited proliferative potential while maintaining the ability to differentiate into any cell type including skeletal muscle cells (SMCs). hPSCs are amenable to genetic editing and can be derived from patient somatic cells, and thus represent a promising option for cell therapies for the treatment of degenerative diseases such as muscular dystrophies. There are unresolved challenges however associated with the derivation and scale-up of hPSCs and generation of differentiated cells in large quantity and high purity. Reported myogenic differentiation protocols are long, require cell sorting and/or rely on ectopic expression of myogenic master regulators. More recent advances have been made with the application of small molecules to enhance the myogenic differentiation efficiency and the identification of more selective markers for the enrichment of myogenic progenitors with enhanced regenerative potential. Here we review the field of myogenic differentiation and highlight areas requiring further research.http://journal.frontiersin.org/Journal/10.3389/fcell.2015.00029/fullMyogenic Regulatory FactorsSatellite Cells, Skeletal MuscleMyogenic Differentiationpluripotentpax7IPSC |
| spellingShingle | Ramzey eAbujarour Bahram eValamehr Generation of skeletal muscle cells from pluripotent stem cells: advances and challenges Frontiers in Cell and Developmental Biology Myogenic Regulatory Factors Satellite Cells, Skeletal Muscle Myogenic Differentiation pluripotent pax7 IPSC |
| title | Generation of skeletal muscle cells from pluripotent stem cells: advances and challenges |
| title_full | Generation of skeletal muscle cells from pluripotent stem cells: advances and challenges |
| title_fullStr | Generation of skeletal muscle cells from pluripotent stem cells: advances and challenges |
| title_full_unstemmed | Generation of skeletal muscle cells from pluripotent stem cells: advances and challenges |
| title_short | Generation of skeletal muscle cells from pluripotent stem cells: advances and challenges |
| title_sort | generation of skeletal muscle cells from pluripotent stem cells advances and challenges |
| topic | Myogenic Regulatory Factors Satellite Cells, Skeletal Muscle Myogenic Differentiation pluripotent pax7 IPSC |
| url | http://journal.frontiersin.org/Journal/10.3389/fcell.2015.00029/full |
| work_keys_str_mv | AT ramzeyeabujarour generationofskeletalmusclecellsfrompluripotentstemcellsadvancesandchallenges AT bahramevalamehr generationofskeletalmusclecellsfrompluripotentstemcellsadvancesandchallenges |