Six New Compounds from the Herbaceous Stems of <i>Ephedra intermedia</i> Schrenket C. A. Meyer and Their Lung-Protective Activity
Six new compounds, (7<i>R</i>,8<i>S</i>,8′<i>R</i>)-balanophorone (<b>1</b>), (7′<i>S</i>,8′<i>R</i>,8<i>R</i>)-yunnanensin A (<b>2</b>), (3<i>S</i>)-thunberginol C (<b>3</b>), (8&...
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MDPI AG
2024-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/2/432 |
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| author | Xiling Fan Yangang Cao Mengnan Zeng Yingjie Ren Xiaoke Zheng Weisheng Feng |
| author_facet | Xiling Fan Yangang Cao Mengnan Zeng Yingjie Ren Xiaoke Zheng Weisheng Feng |
| author_sort | Xiling Fan |
| collection | DOAJ |
| description | Six new compounds, (7<i>R</i>,8<i>S</i>,8′<i>R</i>)-balanophorone (<b>1</b>), (7′<i>S</i>,8′<i>R</i>,8<i>R</i>)-yunnanensin A (<b>2</b>), (3<i>S</i>)-thunberginol C (<b>3</b>), (8<i>R</i>,8′<i>R</i>)-maninsigin B (<b>4</b>), (7<i>S</i>,8<i>R</i>)-4,7,8-dihydroxy-9,9-dimethyl-chroman (<b>5</b>), and 4-hydroxy-1-(4-hydroxy-3-methoxyphenyl)butan-1-one (<b>6</b>), along with eight known compounds (<b>7</b>–<b>14</b>), were isolated from the herbaceous stems of <i>Ephedra intermedia</i> Schrenket C. A. Meyer. Their structures were elucidated based on their spectroscopic (MS, NMR, IR, and UV) data, and their absolute configurations were determined by comparing their calculated and experimental electronic circular dichroic (ECD) spectra. Moreover, compounds <b>1</b> and <b>3</b>–<b>6</b> were evaluated for their ability to protect human pulmonary epithelial cells (BEAS-2B) from injury induced by lipopolysaccharide (LPS) in vitro. The results showed that compound <b>6</b> exhibited a significant protective effect against LPS-induced injury in BEAS-2B, and compound <b>5</b> exhibited a slightly protective effect at the concentration of 10 μM. |
| first_indexed | 2024-03-08T09:50:24Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-08T09:50:24Z |
| publishDate | 2024-01-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-fc74d1cadda944a3a61b142ebd02523f2024-01-29T14:08:34ZengMDPI AGMolecules1420-30492024-01-0129243210.3390/molecules29020432Six New Compounds from the Herbaceous Stems of <i>Ephedra intermedia</i> Schrenket C. A. Meyer and Their Lung-Protective ActivityXiling Fan0Yangang Cao1Mengnan Zeng2Yingjie Ren3Xiaoke Zheng4Weisheng Feng5School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaSchool of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaSchool of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaSchool of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaSchool of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaSchool of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaSix new compounds, (7<i>R</i>,8<i>S</i>,8′<i>R</i>)-balanophorone (<b>1</b>), (7′<i>S</i>,8′<i>R</i>,8<i>R</i>)-yunnanensin A (<b>2</b>), (3<i>S</i>)-thunberginol C (<b>3</b>), (8<i>R</i>,8′<i>R</i>)-maninsigin B (<b>4</b>), (7<i>S</i>,8<i>R</i>)-4,7,8-dihydroxy-9,9-dimethyl-chroman (<b>5</b>), and 4-hydroxy-1-(4-hydroxy-3-methoxyphenyl)butan-1-one (<b>6</b>), along with eight known compounds (<b>7</b>–<b>14</b>), were isolated from the herbaceous stems of <i>Ephedra intermedia</i> Schrenket C. A. Meyer. Their structures were elucidated based on their spectroscopic (MS, NMR, IR, and UV) data, and their absolute configurations were determined by comparing their calculated and experimental electronic circular dichroic (ECD) spectra. Moreover, compounds <b>1</b> and <b>3</b>–<b>6</b> were evaluated for their ability to protect human pulmonary epithelial cells (BEAS-2B) from injury induced by lipopolysaccharide (LPS) in vitro. The results showed that compound <b>6</b> exhibited a significant protective effect against LPS-induced injury in BEAS-2B, and compound <b>5</b> exhibited a slightly protective effect at the concentration of 10 μM.https://www.mdpi.com/1420-3049/29/2/432<i>Ephedra intermedia</i> Schrenket C. A. Meyerchemical componentslung-protective activityMTT assay |
| spellingShingle | Xiling Fan Yangang Cao Mengnan Zeng Yingjie Ren Xiaoke Zheng Weisheng Feng Six New Compounds from the Herbaceous Stems of <i>Ephedra intermedia</i> Schrenket C. A. Meyer and Their Lung-Protective Activity Molecules <i>Ephedra intermedia</i> Schrenket C. A. Meyer chemical components lung-protective activity MTT assay |
| title | Six New Compounds from the Herbaceous Stems of <i>Ephedra intermedia</i> Schrenket C. A. Meyer and Their Lung-Protective Activity |
| title_full | Six New Compounds from the Herbaceous Stems of <i>Ephedra intermedia</i> Schrenket C. A. Meyer and Their Lung-Protective Activity |
| title_fullStr | Six New Compounds from the Herbaceous Stems of <i>Ephedra intermedia</i> Schrenket C. A. Meyer and Their Lung-Protective Activity |
| title_full_unstemmed | Six New Compounds from the Herbaceous Stems of <i>Ephedra intermedia</i> Schrenket C. A. Meyer and Their Lung-Protective Activity |
| title_short | Six New Compounds from the Herbaceous Stems of <i>Ephedra intermedia</i> Schrenket C. A. Meyer and Their Lung-Protective Activity |
| title_sort | six new compounds from the herbaceous stems of i ephedra intermedia i schrenket c a meyer and their lung protective activity |
| topic | <i>Ephedra intermedia</i> Schrenket C. A. Meyer chemical components lung-protective activity MTT assay |
| url | https://www.mdpi.com/1420-3049/29/2/432 |
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