Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung
The division of labor between pulmonary phagocytic subsets [macrophage/monocyte and dendritic cell (DC) subpopulations] has been described at the functional level. However, whether these lung phagocytes also display unique spatial distribution remains unclear. Here, to analyze cellular distribution...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-05-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2018.01046/full |
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| author | Franziska M. Hoffmann Franziska M. Hoffmann Johann L. Berger Johann L. Berger Imke Lingel Imke Lingel Yves Laumonnier Ian P. Lewkowich Ian P. Lewkowich Inken Schmudde Inken Schmudde Peter König Peter König |
| author_facet | Franziska M. Hoffmann Franziska M. Hoffmann Johann L. Berger Johann L. Berger Imke Lingel Imke Lingel Yves Laumonnier Ian P. Lewkowich Ian P. Lewkowich Inken Schmudde Inken Schmudde Peter König Peter König |
| author_sort | Franziska M. Hoffmann |
| collection | DOAJ |
| description | The division of labor between pulmonary phagocytic subsets [macrophage/monocyte and dendritic cell (DC) subpopulations] has been described at the functional level. However, whether these lung phagocytes also display unique spatial distribution remains unclear. Here, to analyze cellular distribution in lung compartments and contacts between phagocyte subpopulations, we established an immunohistochemistry (IHC)-based method to clearly identify murine lung phagocyte subsets in situ based on differential expression of CD11c, CD11b, MHC-II, Langerin and mPDCA-1. Furthermore, we investigated subset-specific functional differences in antigen uptake and spatial changes upon allergic sensitization. Our staining allowed the distinction between alveolar macrophages (AMs), interstitial macrophage (IM) subpopulations, CD11b+ DC subpopulations, CD103+ DCs, and plasmacytoid DCs (pDCs). We identified interstitial regions between airways and around airways as regions of IM/CD11b+ DC/CD103+ DC clusters, where a subset of IMs (IM2) and CD103+ DCs formed intense contacts that decreased upon allergic sensitization. These data indicate functional interactions between both cell types either in steady state or after antigen encounter affecting the development of allergies or tolerance. Furthermore, we observed major antigen uptake in AMs and IMs rather than DC subpopulations that was not restricted to airways and adjacent areas. This will enable to focus future studies to immunologically relevant cellular interactions and to unravel which cells are tipping the balance between pro-inflammatory immune responses or tolerance. |
| first_indexed | 2024-04-12T00:31:34Z |
| format | Article |
| id | doaj.art-fc77606964074defb532bf8287895e61 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T00:31:34Z |
| publishDate | 2018-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-fc77606964074defb532bf8287895e612022-12-22T03:55:19ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-05-01910.3389/fimmu.2018.01046342553Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic LungFranziska M. Hoffmann0Franziska M. Hoffmann1Johann L. Berger2Johann L. Berger3Imke Lingel4Imke Lingel5Yves Laumonnier6Ian P. Lewkowich7Ian P. Lewkowich8Inken Schmudde9Inken Schmudde10Peter König11Peter König12Institute of Anatomy, University of Lübeck, Lübeck, GermanyAirway Research Center North (ARCN), German Center for Lung Research (DZL), Lübeck, GermanyInstitute of Anatomy, University of Lübeck, Lübeck, GermanyAirway Research Center North (ARCN), German Center for Lung Research (DZL), Lübeck, GermanyInstitute of Anatomy, University of Lübeck, Lübeck, GermanyAirway Research Center North (ARCN), German Center for Lung Research (DZL), Lübeck, GermanyInstitute for Systemic Inflammation Research, University of Lübeck, Lübeck, GermanyDivision of Immunobiology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, United StatesDepartment of Pediatrics, University of Cincinnati, Cincinnati, OH, United StatesInstitute of Anatomy, University of Lübeck, Lübeck, GermanyAirway Research Center North (ARCN), German Center for Lung Research (DZL), Lübeck, GermanyInstitute of Anatomy, University of Lübeck, Lübeck, GermanyAirway Research Center North (ARCN), German Center for Lung Research (DZL), Lübeck, GermanyThe division of labor between pulmonary phagocytic subsets [macrophage/monocyte and dendritic cell (DC) subpopulations] has been described at the functional level. However, whether these lung phagocytes also display unique spatial distribution remains unclear. Here, to analyze cellular distribution in lung compartments and contacts between phagocyte subpopulations, we established an immunohistochemistry (IHC)-based method to clearly identify murine lung phagocyte subsets in situ based on differential expression of CD11c, CD11b, MHC-II, Langerin and mPDCA-1. Furthermore, we investigated subset-specific functional differences in antigen uptake and spatial changes upon allergic sensitization. Our staining allowed the distinction between alveolar macrophages (AMs), interstitial macrophage (IM) subpopulations, CD11b+ DC subpopulations, CD103+ DCs, and plasmacytoid DCs (pDCs). We identified interstitial regions between airways and around airways as regions of IM/CD11b+ DC/CD103+ DC clusters, where a subset of IMs (IM2) and CD103+ DCs formed intense contacts that decreased upon allergic sensitization. These data indicate functional interactions between both cell types either in steady state or after antigen encounter affecting the development of allergies or tolerance. Furthermore, we observed major antigen uptake in AMs and IMs rather than DC subpopulations that was not restricted to airways and adjacent areas. This will enable to focus future studies to immunologically relevant cellular interactions and to unravel which cells are tipping the balance between pro-inflammatory immune responses or tolerance.http://journal.frontiersin.org/article/10.3389/fimmu.2018.01046/fulldendritic cellsmacrophageslungimmunohistochemistryspatial distributionantigen uptake |
| spellingShingle | Franziska M. Hoffmann Franziska M. Hoffmann Johann L. Berger Johann L. Berger Imke Lingel Imke Lingel Yves Laumonnier Ian P. Lewkowich Ian P. Lewkowich Inken Schmudde Inken Schmudde Peter König Peter König Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung Frontiers in Immunology dendritic cells macrophages lung immunohistochemistry spatial distribution antigen uptake |
| title | Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung |
| title_full | Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung |
| title_fullStr | Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung |
| title_full_unstemmed | Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung |
| title_short | Distribution and Interaction of Murine Pulmonary Phagocytes in the Naive and Allergic Lung |
| title_sort | distribution and interaction of murine pulmonary phagocytes in the naive and allergic lung |
| topic | dendritic cells macrophages lung immunohistochemistry spatial distribution antigen uptake |
| url | http://journal.frontiersin.org/article/10.3389/fimmu.2018.01046/full |
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