Involvement of the Retinal Pigment Epithelium in the Development of Retinal Lattice Degeneration
Lattice degeneration involves thinning of the retina that occurs over time. Here we performed an immunohistological study of tissue sections of human peripheral retinal lattice degeneration to investigate if retinal pigment epithelium (RPE) cells are involved in the pathogenesis of this condition. I...
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MDPI AG
2020-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/19/7347 |
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| author | Hiroshi Mizuno Masanori Fukumoto Takaki Sato Taeko Horie Teruyo Kida Hidehiro Oku Kimitoshi Nakamura Denan Jin Shinji Takai Tsunehiko Ikeda |
| author_facet | Hiroshi Mizuno Masanori Fukumoto Takaki Sato Taeko Horie Teruyo Kida Hidehiro Oku Kimitoshi Nakamura Denan Jin Shinji Takai Tsunehiko Ikeda |
| author_sort | Hiroshi Mizuno |
| collection | DOAJ |
| description | Lattice degeneration involves thinning of the retina that occurs over time. Here we performed an immunohistological study of tissue sections of human peripheral retinal lattice degeneration to investigate if retinal pigment epithelium (RPE) cells are involved in the pathogenesis of this condition. In two cases of retinal detachment with a large tear that underwent vitreous surgery, retinal lattice degeneration tissue specimens were collected during surgery. In the obtained specimens, both whole mounts and horizontal section slices were prepared, and immunostaining was then performed with hematoxylin and antibodies against glial fibrillary acidic protein (GFAP), RPE-specific protein 65 kDa (RPE65), pan-cytokeratin (pan-CK), and CK18. Hematoxylin staining showed no nuclei in the center of the degenerative lesion, thus suggesting the possibility of the occurrence of apoptosis. In the degenerative lesion specimens, GFAP staining was observed in the center, RPE65 staining was observed in the slightly peripheral region, and pan-CK staining was observed in all areas. However, no obvious CK18 staining was observed. In a monkey retina used as the control specimen of a normal healthy retina, no RPE65 or pan-CK staining was observed in the neural retina. Our findings suggest that migration, proliferation, and differentiation of RPE cells might be involved in the repair of retinal lattice degeneration. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T15:51:21Z |
| publishDate | 2020-10-01 |
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| spelling | doaj.art-fc77a21c3a4d4615891ce61cf5304a772023-11-20T16:06:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-012119734710.3390/ijms21197347Involvement of the Retinal Pigment Epithelium in the Development of Retinal Lattice DegenerationHiroshi Mizuno0Masanori Fukumoto1Takaki Sato2Taeko Horie3Teruyo Kida4Hidehiro Oku5Kimitoshi Nakamura6Denan Jin7Shinji Takai8Tsunehiko Ikeda9Department of Ophthalmology, Osaka Medical College, Takatsuki-City, Osaka 569-8686, JapanDepartment of Ophthalmology, Osaka Medical College, Takatsuki-City, Osaka 569-8686, JapanDepartment of Ophthalmology, Osaka Medical College, Takatsuki-City, Osaka 569-8686, JapanDepartment of Ophthalmology, Osaka Medical College, Takatsuki-City, Osaka 569-8686, JapanDepartment of Ophthalmology, Osaka Medical College, Takatsuki-City, Osaka 569-8686, JapanDepartment of Ophthalmology, Osaka Medical College, Takatsuki-City, Osaka 569-8686, JapanNakamura Eye Clinic, Matsumoto-City, Nagano 390-0811, JapanDepartment of Innovative Medicine, Graduate School of Medicine, Osaka Medical College, Takatsuki-City, Osaka 569-8686, JapanDepartment of Innovative Medicine, Graduate School of Medicine, Osaka Medical College, Takatsuki-City, Osaka 569-8686, JapanDepartment of Ophthalmology, Osaka Medical College, Takatsuki-City, Osaka 569-8686, JapanLattice degeneration involves thinning of the retina that occurs over time. Here we performed an immunohistological study of tissue sections of human peripheral retinal lattice degeneration to investigate if retinal pigment epithelium (RPE) cells are involved in the pathogenesis of this condition. In two cases of retinal detachment with a large tear that underwent vitreous surgery, retinal lattice degeneration tissue specimens were collected during surgery. In the obtained specimens, both whole mounts and horizontal section slices were prepared, and immunostaining was then performed with hematoxylin and antibodies against glial fibrillary acidic protein (GFAP), RPE-specific protein 65 kDa (RPE65), pan-cytokeratin (pan-CK), and CK18. Hematoxylin staining showed no nuclei in the center of the degenerative lesion, thus suggesting the possibility of the occurrence of apoptosis. In the degenerative lesion specimens, GFAP staining was observed in the center, RPE65 staining was observed in the slightly peripheral region, and pan-CK staining was observed in all areas. However, no obvious CK18 staining was observed. In a monkey retina used as the control specimen of a normal healthy retina, no RPE65 or pan-CK staining was observed in the neural retina. Our findings suggest that migration, proliferation, and differentiation of RPE cells might be involved in the repair of retinal lattice degeneration.https://www.mdpi.com/1422-0067/21/19/7347retinal lattice degenerationretinal detachmentretinal pigment epithelium (RPE)glial fibrillary acidic protein (GFAP)retinal pigment epithelium-specific protein 65 kDa (RPE65)cytokeratin (CK) |
| spellingShingle | Hiroshi Mizuno Masanori Fukumoto Takaki Sato Taeko Horie Teruyo Kida Hidehiro Oku Kimitoshi Nakamura Denan Jin Shinji Takai Tsunehiko Ikeda Involvement of the Retinal Pigment Epithelium in the Development of Retinal Lattice Degeneration International Journal of Molecular Sciences retinal lattice degeneration retinal detachment retinal pigment epithelium (RPE) glial fibrillary acidic protein (GFAP) retinal pigment epithelium-specific protein 65 kDa (RPE65) cytokeratin (CK) |
| title | Involvement of the Retinal Pigment Epithelium in the Development of Retinal Lattice Degeneration |
| title_full | Involvement of the Retinal Pigment Epithelium in the Development of Retinal Lattice Degeneration |
| title_fullStr | Involvement of the Retinal Pigment Epithelium in the Development of Retinal Lattice Degeneration |
| title_full_unstemmed | Involvement of the Retinal Pigment Epithelium in the Development of Retinal Lattice Degeneration |
| title_short | Involvement of the Retinal Pigment Epithelium in the Development of Retinal Lattice Degeneration |
| title_sort | involvement of the retinal pigment epithelium in the development of retinal lattice degeneration |
| topic | retinal lattice degeneration retinal detachment retinal pigment epithelium (RPE) glial fibrillary acidic protein (GFAP) retinal pigment epithelium-specific protein 65 kDa (RPE65) cytokeratin (CK) |
| url | https://www.mdpi.com/1422-0067/21/19/7347 |
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