Characterization of the Antiproliferative Activity of <em>Sargassum muticum</em> Low and High Molecular Weight Polysaccharide Fractions
The extract obtained by pressurized hot water extraction from <i>Sargassum muticum</i>, to recover the bioactive compound known as fucoidan, was fractionated using membranes of 100, 50, 30, 10, and 5 kDa, obtaining five retentates and the final permeate. These fractions were characterize...
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MDPI AG
2023-12-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/22/1/16 |
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| author | Lara Diego-González Milena Álvarez-Viñas Rosana Simón-Vázquez Herminia Domínguez Maria Dolores Torres Noelia Flórez-Fernández |
| author_facet | Lara Diego-González Milena Álvarez-Viñas Rosana Simón-Vázquez Herminia Domínguez Maria Dolores Torres Noelia Flórez-Fernández |
| author_sort | Lara Diego-González |
| collection | DOAJ |
| description | The extract obtained by pressurized hot water extraction from <i>Sargassum muticum</i>, to recover the bioactive compound known as fucoidan, was fractionated using membranes of 100, 50, 30, 10, and 5 kDa, obtaining five retentates and the final permeate. These fractions were characterized for phloroglucinol content, protein content, sulfate content, and trolox equivalent antioxidant capacity (TEAC); apart from oligosaccharides, FTIR and molar mass distribution were also evaluated. Retentates of 100 and 50 kDa showed higher values for phloroglucinol, TEAC, and sulfate content. The rheology of the alginate fraction was also evaluated. Regarding the potential antitumoral activity, all fractions were assessed in MCF-7 cells using a metabolic activity assay based on the reduction of a tetrazolium compound, the most efficient being R100 and R50. Based on the results, these fractions were compared with commercial fucoidans at the same concentrations, and similar results were found. In addition, synergistic cytotoxic effects using two drugs commonly used in breast cancer, cis-Platinum (cis-Pt) and 5-fluorouracil (5-FU), were tested in combination with R100 and R50. Promising results were obtained when the retentate and the drugs were mixed, showing an improvement in the cytotoxicity induced by the chemotherapy. |
| first_indexed | 2024-03-08T10:43:55Z |
| format | Article |
| id | doaj.art-fc79669299424effbeaf04794b5d0863 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-08T10:43:55Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-fc79669299424effbeaf04794b5d08632024-01-26T17:25:55ZengMDPI AGMarine Drugs1660-33972023-12-012211610.3390/md22010016Characterization of the Antiproliferative Activity of <em>Sargassum muticum</em> Low and High Molecular Weight Polysaccharide FractionsLara Diego-González0Milena Álvarez-Viñas1Rosana Simón-Vázquez2Herminia Domínguez3Maria Dolores Torres4Noelia Flórez-Fernández5CINBIO, Universidade de Vigo, Grupo Inmunología, 36310 Vigo, SpainCINBIO, Universidade de Vigo, Grupo Biomasa y Desarrollo Sostenible, 32004 Ourense, SpainCINBIO, Universidade de Vigo, Grupo Inmunología, 36310 Vigo, SpainCINBIO, Universidade de Vigo, Grupo Biomasa y Desarrollo Sostenible, 32004 Ourense, SpainCINBIO, Universidade de Vigo, Grupo Biomasa y Desarrollo Sostenible, 32004 Ourense, SpainCINBIO, Universidade de Vigo, Grupo Biomasa y Desarrollo Sostenible, 32004 Ourense, SpainThe extract obtained by pressurized hot water extraction from <i>Sargassum muticum</i>, to recover the bioactive compound known as fucoidan, was fractionated using membranes of 100, 50, 30, 10, and 5 kDa, obtaining five retentates and the final permeate. These fractions were characterized for phloroglucinol content, protein content, sulfate content, and trolox equivalent antioxidant capacity (TEAC); apart from oligosaccharides, FTIR and molar mass distribution were also evaluated. Retentates of 100 and 50 kDa showed higher values for phloroglucinol, TEAC, and sulfate content. The rheology of the alginate fraction was also evaluated. Regarding the potential antitumoral activity, all fractions were assessed in MCF-7 cells using a metabolic activity assay based on the reduction of a tetrazolium compound, the most efficient being R100 and R50. Based on the results, these fractions were compared with commercial fucoidans at the same concentrations, and similar results were found. In addition, synergistic cytotoxic effects using two drugs commonly used in breast cancer, cis-Platinum (cis-Pt) and 5-fluorouracil (5-FU), were tested in combination with R100 and R50. Promising results were obtained when the retentate and the drugs were mixed, showing an improvement in the cytotoxicity induced by the chemotherapy.https://www.mdpi.com/1660-3397/22/1/16antitumoralpressurized hot waterfucoidanfractionationalginaterheology |
| spellingShingle | Lara Diego-González Milena Álvarez-Viñas Rosana Simón-Vázquez Herminia Domínguez Maria Dolores Torres Noelia Flórez-Fernández Characterization of the Antiproliferative Activity of <em>Sargassum muticum</em> Low and High Molecular Weight Polysaccharide Fractions Marine Drugs antitumoral pressurized hot water fucoidan fractionation alginate rheology |
| title | Characterization of the Antiproliferative Activity of <em>Sargassum muticum</em> Low and High Molecular Weight Polysaccharide Fractions |
| title_full | Characterization of the Antiproliferative Activity of <em>Sargassum muticum</em> Low and High Molecular Weight Polysaccharide Fractions |
| title_fullStr | Characterization of the Antiproliferative Activity of <em>Sargassum muticum</em> Low and High Molecular Weight Polysaccharide Fractions |
| title_full_unstemmed | Characterization of the Antiproliferative Activity of <em>Sargassum muticum</em> Low and High Molecular Weight Polysaccharide Fractions |
| title_short | Characterization of the Antiproliferative Activity of <em>Sargassum muticum</em> Low and High Molecular Weight Polysaccharide Fractions |
| title_sort | characterization of the antiproliferative activity of em sargassum muticum em low and high molecular weight polysaccharide fractions |
| topic | antitumoral pressurized hot water fucoidan fractionation alginate rheology |
| url | https://www.mdpi.com/1660-3397/22/1/16 |
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