Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients
Osteosarcoma (OS) is a rare malignant primary bone tumours characterized by a high genetic and cell composition heterogeneity. Unfortunately, despite the use of drug combinations and the recent development of immunotherapies, the overall survival has not improved in the last four decades. Due to the...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Journal of Bone Oncology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212137422000410 |
| _version_ | 1798020774832898048 |
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| author | Clément J.F. Heymann Christine Bobin-Dubigeon Javier Muñoz-Garcia Denis Cochonneau Emilie Ollivier Marie-Françoise Heymann Dominique Heymann |
| author_facet | Clément J.F. Heymann Christine Bobin-Dubigeon Javier Muñoz-Garcia Denis Cochonneau Emilie Ollivier Marie-Françoise Heymann Dominique Heymann |
| author_sort | Clément J.F. Heymann |
| collection | DOAJ |
| description | Osteosarcoma (OS) is a rare malignant primary bone tumours characterized by a high genetic and cell composition heterogeneity. Unfortunately, despite the use of drug combinations and the recent development of immunotherapies, the overall survival has not improved in the last four decades. Due to the key role of the tumour microenvironment in the pathogenesis of OS, a better understanding of its microenvironment is mandatory to develop new therapeutic approaches. From retrospective biological cohorts of OS, we analysed by immunohistochemistry the presence of lipopolysaccharide (LPS)-binding protein (LBP) in diagnostic biopsies with local disease and compared their level of infiltration to patients suffering from metastatic status. LBP is considered as a marker of LPS exposure and can indirectly reflect the presence of Gram-negative microbiota. LBP were detected in the cytoplasm of OS cells as well as in tumour-associated macrophage. Tumour samples of patients with local disease were significantly enriched in LBP compared to tumour tissues of patients with metastatic status. Lung metastatic tissues showed similar level of LBP compared to paired primary tumours. Overall, this study strongly suggests the presence of Gram-negative bacteria in OS tissues and demonstrated their significant differential level according the metastatic status. This tumour-associated microbiome may help in the conceptualisation of new therapeutic approach to trigger efficient therapeutic responses against cancer. |
| first_indexed | 2024-04-11T17:03:07Z |
| format | Article |
| id | doaj.art-fc7bf4d197b84970a04d270624959e68 |
| institution | Directory Open Access Journal |
| issn | 2212-1374 |
| language | English |
| last_indexed | 2024-04-11T17:03:07Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Bone Oncology |
| spelling | doaj.art-fc7bf4d197b84970a04d270624959e682022-12-22T04:13:07ZengElsevierJournal of Bone Oncology2212-13742022-10-0136100451Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patientsClément J.F. Heymann0Christine Bobin-Dubigeon1Javier Muñoz-Garcia2Denis Cochonneau3Emilie Ollivier4Marie-Françoise Heymann5Dominique Heymann6University of Amsterdam, Academic Medical Center, Swammerdam Institute for Life Sciences, Amsterdam, the Netherlands; Institut de Cancérologie de l’Ouest, Tumour Heterogeneity and Precison Medicine Laboratory, Saint-Herblain, FranceInstitut de Cancérologie de l’Ouest, Tumour Heterogeneity and Precison Medicine Laboratory, Saint-Herblain, France; Nantes Université, CNRS, UMR6286, US2B, Nantes, FranceInstitut de Cancérologie de l’Ouest, Tumour Heterogeneity and Precison Medicine Laboratory, Saint-Herblain, FranceInstitut de Cancérologie de l’Ouest, Tumour Heterogeneity and Precison Medicine Laboratory, Saint-Herblain, FranceInstitut de Cancérologie de l’Ouest, Tumour Heterogeneity and Precison Medicine Laboratory, Saint-Herblain, FranceInstitut de Cancérologie de l’Ouest, Tumour Heterogeneity and Precison Medicine Laboratory, Saint-Herblain, France; Institut de Cancérologie de l’Ouest, Research Pathology Platform, Saint-Herblain, France; Corresponding authors at: Institut de Cancérologie de l’Ouest, Blvd Jacques Monod, 44805 Saint-Herblain, France.Institut de Cancérologie de l’Ouest, Tumour Heterogeneity and Precison Medicine Laboratory, Saint-Herblain, France; Nantes Université, CNRS, UMR6286, US2B, Nantes, France; University of Sheffield, Department of Oncology and Metabolism, Medical School, Sheffield, UK; Corresponding authors at: Institut de Cancérologie de l’Ouest, Blvd Jacques Monod, 44805 Saint-Herblain, France.Osteosarcoma (OS) is a rare malignant primary bone tumours characterized by a high genetic and cell composition heterogeneity. Unfortunately, despite the use of drug combinations and the recent development of immunotherapies, the overall survival has not improved in the last four decades. Due to the key role of the tumour microenvironment in the pathogenesis of OS, a better understanding of its microenvironment is mandatory to develop new therapeutic approaches. From retrospective biological cohorts of OS, we analysed by immunohistochemistry the presence of lipopolysaccharide (LPS)-binding protein (LBP) in diagnostic biopsies with local disease and compared their level of infiltration to patients suffering from metastatic status. LBP is considered as a marker of LPS exposure and can indirectly reflect the presence of Gram-negative microbiota. LBP were detected in the cytoplasm of OS cells as well as in tumour-associated macrophage. Tumour samples of patients with local disease were significantly enriched in LBP compared to tumour tissues of patients with metastatic status. Lung metastatic tissues showed similar level of LBP compared to paired primary tumours. Overall, this study strongly suggests the presence of Gram-negative bacteria in OS tissues and demonstrated their significant differential level according the metastatic status. This tumour-associated microbiome may help in the conceptualisation of new therapeutic approach to trigger efficient therapeutic responses against cancer.http://www.sciencedirect.com/science/article/pii/S2212137422000410OsteosarcomaIntratumour bacteriaGram-negative microbiotaTumour-associated macrophages |
| spellingShingle | Clément J.F. Heymann Christine Bobin-Dubigeon Javier Muñoz-Garcia Denis Cochonneau Emilie Ollivier Marie-Françoise Heymann Dominique Heymann Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients Journal of Bone Oncology Osteosarcoma Intratumour bacteria Gram-negative microbiota Tumour-associated macrophages |
| title | Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
| title_full | Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
| title_fullStr | Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
| title_full_unstemmed | Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
| title_short | Lipopolysaccharide-binding protein expression is associated to the metastatic status of osteosarcoma patients |
| title_sort | lipopolysaccharide binding protein expression is associated to the metastatic status of osteosarcoma patients |
| topic | Osteosarcoma Intratumour bacteria Gram-negative microbiota Tumour-associated macrophages |
| url | http://www.sciencedirect.com/science/article/pii/S2212137422000410 |
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