The role of Na<sup>+</sup>/H<sup>+ </sup>exchanger in Ca<sup>2+ </sup>overload and ischemic myocardial damage in hearts from type 2 diabetic <it>db/db </it>mice
<p>Abstract</p> <p>Background</p> <p>A higher increase in intracellular Na<sup>+ </sup>via Na<sup>+</sup>/H<sup>+ </sup>exchanger (NHE) during ischemia has been reported in type 2 diabetic mouse hearts. We investigated the role of NHE...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-04-01
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| Series: | Cardiovascular Diabetology |
| Online Access: | http://www.cardiab.com/content/11/1/33 |
| _version_ | 1818210981987221504 |
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| author | Anzawa Ryuko Seki Shingo Nagoshi Tomohisa Taniguchi Ikuo Feuvray Danielle Yoshimura Michihiro |
| author_facet | Anzawa Ryuko Seki Shingo Nagoshi Tomohisa Taniguchi Ikuo Feuvray Danielle Yoshimura Michihiro |
| author_sort | Anzawa Ryuko |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>A higher increase in intracellular Na<sup>+ </sup>via Na<sup>+</sup>/H<sup>+ </sup>exchanger (NHE) during ischemia has been reported in type 2 diabetic mouse hearts. We investigated the role of NHE in inducing changes in cytoplasmic Ca<sup>2+ </sup>concentration ([Ca<sup>2+</sup>]<sub>i</sub>) and alterations in ventricular function during ischemia-reperfusion in type 2 diabetic mouse hearts.</p> <p>Methods</p> <p>Hearts from male type 2 diabetic <it>db/db </it>(12-15 weeks old) and age-matched control <it>db/+ </it>mice were subjected to Langendorff perfusion and loaded with 4μM of the Ca<sup>2+ </sup>indicator fura-2. The hearts were exposed to no-flow ischemia for 15 minutes and then reperfused. [Ca<sup>2+</sup>]<sub>i </sub>was measured by monitoring fura-2 fluorescence at 500 nm (excitation wavelengths of 340 and 380 nm), while left ventricular (LV) pressure was simultaneously measured.</p> <p>Results</p> <p><it>db/db </it>hearts exhibited a lower recovery of LV developed pressure than <it>db/+ </it>hearts during reperfusion following ischemia. Diastolic [Ca<sup>2+</sup>]<sub>i </sub>was increased to a greater level in diabetic hearts than in the control hearts during ischemia and reperfusion. Such an increase in cytoplasmic Ca<sup>2+ </sup>overload during ischemia-reperfusion in diabetic hearts was markedly reduced in the presence of the NHE inhibitor cariporide. This was accompanied by a significantly improved recovery of ventricular function on reperfusion, as shown by a lower increase in diastolic pressure and increased recovery of developed pressure.</p> <p>Conclusion</p> <p>NHE plays a key role in enhancing cytoplasmic Ca<sup>2+ </sup>overload during ischemia-reperfusion and severely impairing post-ischemic cardiac function in hearts from type 2 diabetic <it>db/db </it>mice.</p> |
| first_indexed | 2024-12-12T05:25:15Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1475-2840 |
| language | English |
| last_indexed | 2024-12-12T05:25:15Z |
| publishDate | 2012-04-01 |
| publisher | BMC |
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| series | Cardiovascular Diabetology |
| spelling | doaj.art-fc7eb2b3ed2c45e9b49137056d91e9f02022-12-22T00:36:30ZengBMCCardiovascular Diabetology1475-28402012-04-011113310.1186/1475-2840-11-33The role of Na<sup>+</sup>/H<sup>+ </sup>exchanger in Ca<sup>2+ </sup>overload and ischemic myocardial damage in hearts from type 2 diabetic <it>db/db </it>miceAnzawa RyukoSeki ShingoNagoshi TomohisaTaniguchi IkuoFeuvray DanielleYoshimura Michihiro<p>Abstract</p> <p>Background</p> <p>A higher increase in intracellular Na<sup>+ </sup>via Na<sup>+</sup>/H<sup>+ </sup>exchanger (NHE) during ischemia has been reported in type 2 diabetic mouse hearts. We investigated the role of NHE in inducing changes in cytoplasmic Ca<sup>2+ </sup>concentration ([Ca<sup>2+</sup>]<sub>i</sub>) and alterations in ventricular function during ischemia-reperfusion in type 2 diabetic mouse hearts.</p> <p>Methods</p> <p>Hearts from male type 2 diabetic <it>db/db </it>(12-15 weeks old) and age-matched control <it>db/+ </it>mice were subjected to Langendorff perfusion and loaded with 4μM of the Ca<sup>2+ </sup>indicator fura-2. The hearts were exposed to no-flow ischemia for 15 minutes and then reperfused. [Ca<sup>2+</sup>]<sub>i </sub>was measured by monitoring fura-2 fluorescence at 500 nm (excitation wavelengths of 340 and 380 nm), while left ventricular (LV) pressure was simultaneously measured.</p> <p>Results</p> <p><it>db/db </it>hearts exhibited a lower recovery of LV developed pressure than <it>db/+ </it>hearts during reperfusion following ischemia. Diastolic [Ca<sup>2+</sup>]<sub>i </sub>was increased to a greater level in diabetic hearts than in the control hearts during ischemia and reperfusion. Such an increase in cytoplasmic Ca<sup>2+ </sup>overload during ischemia-reperfusion in diabetic hearts was markedly reduced in the presence of the NHE inhibitor cariporide. This was accompanied by a significantly improved recovery of ventricular function on reperfusion, as shown by a lower increase in diastolic pressure and increased recovery of developed pressure.</p> <p>Conclusion</p> <p>NHE plays a key role in enhancing cytoplasmic Ca<sup>2+ </sup>overload during ischemia-reperfusion and severely impairing post-ischemic cardiac function in hearts from type 2 diabetic <it>db/db </it>mice.</p>http://www.cardiab.com/content/11/1/33 |
| spellingShingle | Anzawa Ryuko Seki Shingo Nagoshi Tomohisa Taniguchi Ikuo Feuvray Danielle Yoshimura Michihiro The role of Na<sup>+</sup>/H<sup>+ </sup>exchanger in Ca<sup>2+ </sup>overload and ischemic myocardial damage in hearts from type 2 diabetic <it>db/db </it>mice Cardiovascular Diabetology |
| title | The role of Na<sup>+</sup>/H<sup>+ </sup>exchanger in Ca<sup>2+ </sup>overload and ischemic myocardial damage in hearts from type 2 diabetic <it>db/db </it>mice |
| title_full | The role of Na<sup>+</sup>/H<sup>+ </sup>exchanger in Ca<sup>2+ </sup>overload and ischemic myocardial damage in hearts from type 2 diabetic <it>db/db </it>mice |
| title_fullStr | The role of Na<sup>+</sup>/H<sup>+ </sup>exchanger in Ca<sup>2+ </sup>overload and ischemic myocardial damage in hearts from type 2 diabetic <it>db/db </it>mice |
| title_full_unstemmed | The role of Na<sup>+</sup>/H<sup>+ </sup>exchanger in Ca<sup>2+ </sup>overload and ischemic myocardial damage in hearts from type 2 diabetic <it>db/db </it>mice |
| title_short | The role of Na<sup>+</sup>/H<sup>+ </sup>exchanger in Ca<sup>2+ </sup>overload and ischemic myocardial damage in hearts from type 2 diabetic <it>db/db </it>mice |
| title_sort | role of na sup sup h sup sup exchanger in ca sup 2 sup overload and ischemic myocardial damage in hearts from type 2 diabetic it db db it mice |
| url | http://www.cardiab.com/content/11/1/33 |
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