Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical Artery
The gold standard vascular substitutes, used in cardiovascular surgery, are the Dacron or expanded polytetrafluoroethylene (ePTFE)-derived grafts. However, major adverse reactions accompany their use. For this purpose, decellularized human umbilical arteries (hUAs) may be proven as a significant sou...
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| Format: | Article |
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MDPI AG
2020-08-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/8/8/280 |
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| author | Panagiotis Mallis Dimitrios P. Sokolis Manousos Makridakis Jerome Zoidakis Athanasios D. Velentzas Michalis Katsimpoulas Antonia Vlahou Alkiviadis Kostakis Catherine Stavropoulos-Giokas Efstathios Michalopoulos |
| author_facet | Panagiotis Mallis Dimitrios P. Sokolis Manousos Makridakis Jerome Zoidakis Athanasios D. Velentzas Michalis Katsimpoulas Antonia Vlahou Alkiviadis Kostakis Catherine Stavropoulos-Giokas Efstathios Michalopoulos |
| author_sort | Panagiotis Mallis |
| collection | DOAJ |
| description | The gold standard vascular substitutes, used in cardiovascular surgery, are the Dacron or expanded polytetrafluoroethylene (ePTFE)-derived grafts. However, major adverse reactions accompany their use. For this purpose, decellularized human umbilical arteries (hUAs) may be proven as a significant source for the development of small diameter conduits. The aim of this study was the evaluation of a decellularization protocol in hUAs. To study the effect of the decellularization to the hUAs, histological analysis was performed. Then, native and decellularized hUAs were biochemically and biomechanically evaluated. Finally, broad proteomic analysis was applied. Histological analysis revealed the successful decellularization of the hUAs. Furthermore, a great amount of DNA was removed from the decellularized hUAs. Biomechanical analysis revealed statistically significant differences in longitudinal direction only in maximum stress (<i>p</i> < 0.013) and strain (<i>p</i> < 0.001). On the contrary, all parameters tested for circumferential direction exhibited significant differences (<i>p</i> < 0.05). Proteomic analysis showed the preservation of the extracellular matrix and cytoskeletal proteins in both groups. Proteomic data are available via ProteomeXchange with identifier PXD020187. The above results indicated that hUAs were efficiently decellularized. The tissue function properties of these conduits were well retained, making them ideal candidates for the development of small diameter vascular grafts. |
| first_indexed | 2024-03-10T17:40:23Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T17:40:23Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-fc7ef99382f24e42881a35e4477018db2023-11-20T09:43:33ZengMDPI AGBiomedicines2227-90592020-08-018828010.3390/biomedicines8080280Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical ArteryPanagiotis Mallis0Dimitrios P. Sokolis1Manousos Makridakis2Jerome Zoidakis3Athanasios D. Velentzas4Michalis Katsimpoulas5Antonia Vlahou6Alkiviadis Kostakis7Catherine Stavropoulos-Giokas8Efstathios Michalopoulos9Hellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, GreeceLaboratory of Biomechanics, Center for Experimental Surgery, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, GreeceBiotechnology division, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, GreeceBiotechnology division, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, GreeceDepartment of Biology, Section of Cell Biology and Biophysics, School of Science, National and Kapodistrian University of Athens, 161 Gr. Kousidi, Zografos, Street, 115 27 Athens, GreeceCenter of Experimental Surgery and Translational Research, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, GreeceBiotechnology division, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, GreeceCenter of Experimental Surgery and Translational Research, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, GreeceHellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, GreeceHellenic Cord Blood Bank, Biomedical Research Foundation Academy of Athens, 4 Soranou Ephessiou Street, 115 27 Athens, GreeceThe gold standard vascular substitutes, used in cardiovascular surgery, are the Dacron or expanded polytetrafluoroethylene (ePTFE)-derived grafts. However, major adverse reactions accompany their use. For this purpose, decellularized human umbilical arteries (hUAs) may be proven as a significant source for the development of small diameter conduits. The aim of this study was the evaluation of a decellularization protocol in hUAs. To study the effect of the decellularization to the hUAs, histological analysis was performed. Then, native and decellularized hUAs were biochemically and biomechanically evaluated. Finally, broad proteomic analysis was applied. Histological analysis revealed the successful decellularization of the hUAs. Furthermore, a great amount of DNA was removed from the decellularized hUAs. Biomechanical analysis revealed statistically significant differences in longitudinal direction only in maximum stress (<i>p</i> < 0.013) and strain (<i>p</i> < 0.001). On the contrary, all parameters tested for circumferential direction exhibited significant differences (<i>p</i> < 0.05). Proteomic analysis showed the preservation of the extracellular matrix and cytoskeletal proteins in both groups. Proteomic data are available via ProteomeXchange with identifier PXD020187. The above results indicated that hUAs were efficiently decellularized. The tissue function properties of these conduits were well retained, making them ideal candidates for the development of small diameter vascular grafts.https://www.mdpi.com/2227-9059/8/8/280human umbilical arteriesdecellularizationDNAbiomechanical analysisLC/MScollagen |
| spellingShingle | Panagiotis Mallis Dimitrios P. Sokolis Manousos Makridakis Jerome Zoidakis Athanasios D. Velentzas Michalis Katsimpoulas Antonia Vlahou Alkiviadis Kostakis Catherine Stavropoulos-Giokas Efstathios Michalopoulos Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical Artery Biomedicines human umbilical arteries decellularization DNA biomechanical analysis LC/MS collagen |
| title | Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical Artery |
| title_full | Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical Artery |
| title_fullStr | Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical Artery |
| title_full_unstemmed | Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical Artery |
| title_short | Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical Artery |
| title_sort | insights into biomechanical and proteomic characteristics of small diameter vascular grafts utilizing the human umbilical artery |
| topic | human umbilical arteries decellularization DNA biomechanical analysis LC/MS collagen |
| url | https://www.mdpi.com/2227-9059/8/8/280 |
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