The vital role of covS in the establishment of Streptococcus equi subsp. zooepidemicus virulence
Streptococcus equi subsp. zooepidemicus (SEZ) is an important zoonotic agent. Here, a virulence-attenuated strain M35246 derived from natural variation of wild-type SEZ ATCC35246 was found. M35246 showed a deletion of 25 contiguous genes as well as a loss-of-function mutation in covS. Subsequently,...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311922001873 |
| _version_ | 1811176483777937408 |
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| author | Bin XU Zhe MA Hong ZHOU Hui-xing LIN Hong-jie FAN |
| author_facet | Bin XU Zhe MA Hong ZHOU Hui-xing LIN Hong-jie FAN |
| author_sort | Bin XU |
| collection | DOAJ |
| description | Streptococcus equi subsp. zooepidemicus (SEZ) is an important zoonotic agent. Here, a virulence-attenuated strain M35246 derived from natural variation of wild-type SEZ ATCC35246 was found. M35246 showed a deletion of 25 contiguous genes as well as a loss-of-function mutation in covS. Subsequently, a 25-gene-deleted strain (ΔPI), a covS-mutant strain (McovS), and relevant complementary strains were constructed and investigated. M35246 and McovS were significantly less encapsulated and exhibited poorer anti-phagocytic capacity compared to wild-type SEZ. McovS was significantly more sensitive to β-lactams, aminoglycosides, macrolides, and lincosamides than wild-type SEZ. M35246, McovS, and ΔPI exhibited an increase in median lethal dose (LD50) in mice by 105, 105, and 5 times when compared to wild-type SEZ, respectively. Neither M35246 nor McovS were isolated from mice 48 h after being challenged with approximately 2 000 times the LD50 of wild-type SEZ. Transcriptome analysis showed that 668 significantly differentially expressed genes existed between McovS and wild-type SEZ. Numerous virulence factor-encoding genes and anabolic-related genes in McovS that were involved in anti-phagocytosis, capsule formation, pathogenicity, and antibiotic resistance were downregulated significantly relative to the wild-type strain. This study revealed that the CovS plays a vital role in the establishment of SEZ virulence. |
| first_indexed | 2024-04-10T19:52:20Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-04-10T19:52:20Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-fc7f0a9da80845b5935082bc258e9bfd2023-01-28T04:07:38ZengElsevierJournal of Integrative Agriculture2095-31192023-02-01222568584The vital role of covS in the establishment of Streptococcus equi subsp. zooepidemicus virulenceBin XU0Zhe MA1Hong ZHOU2Hui-xing LIN3Hong-jie FAN4MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China; Key Laboratory of Veterinary Biological Engineering and Technology of Ministry of Agriculture and Rural Affairs, National Research Center of Veterinary Biologicals Engineering and Technology, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, P.R.ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China; Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, P.R.ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China; Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, P.R.China; Correspondence FAN Hong-jie, Tel/Fax: +86-25-84399592Streptococcus equi subsp. zooepidemicus (SEZ) is an important zoonotic agent. Here, a virulence-attenuated strain M35246 derived from natural variation of wild-type SEZ ATCC35246 was found. M35246 showed a deletion of 25 contiguous genes as well as a loss-of-function mutation in covS. Subsequently, a 25-gene-deleted strain (ΔPI), a covS-mutant strain (McovS), and relevant complementary strains were constructed and investigated. M35246 and McovS were significantly less encapsulated and exhibited poorer anti-phagocytic capacity compared to wild-type SEZ. McovS was significantly more sensitive to β-lactams, aminoglycosides, macrolides, and lincosamides than wild-type SEZ. M35246, McovS, and ΔPI exhibited an increase in median lethal dose (LD50) in mice by 105, 105, and 5 times when compared to wild-type SEZ, respectively. Neither M35246 nor McovS were isolated from mice 48 h after being challenged with approximately 2 000 times the LD50 of wild-type SEZ. Transcriptome analysis showed that 668 significantly differentially expressed genes existed between McovS and wild-type SEZ. Numerous virulence factor-encoding genes and anabolic-related genes in McovS that were involved in anti-phagocytosis, capsule formation, pathogenicity, and antibiotic resistance were downregulated significantly relative to the wild-type strain. This study revealed that the CovS plays a vital role in the establishment of SEZ virulence.http://www.sciencedirect.com/science/article/pii/S2095311922001873Streptococcus equi subsp. zooepidemicuscovSnatural variationvirulenceregulation |
| spellingShingle | Bin XU Zhe MA Hong ZHOU Hui-xing LIN Hong-jie FAN The vital role of covS in the establishment of Streptococcus equi subsp. zooepidemicus virulence Journal of Integrative Agriculture Streptococcus equi subsp. zooepidemicus covS natural variation virulence regulation |
| title | The vital role of covS in the establishment of Streptococcus equi subsp. zooepidemicus virulence |
| title_full | The vital role of covS in the establishment of Streptococcus equi subsp. zooepidemicus virulence |
| title_fullStr | The vital role of covS in the establishment of Streptococcus equi subsp. zooepidemicus virulence |
| title_full_unstemmed | The vital role of covS in the establishment of Streptococcus equi subsp. zooepidemicus virulence |
| title_short | The vital role of covS in the establishment of Streptococcus equi subsp. zooepidemicus virulence |
| title_sort | vital role of covs in the establishment of streptococcus equi subsp zooepidemicus virulence |
| topic | Streptococcus equi subsp. zooepidemicus covS natural variation virulence regulation |
| url | http://www.sciencedirect.com/science/article/pii/S2095311922001873 |
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