Functional redundancy of OsPIN1 paralogous genes in regulating plant growth and development in rice
The OsPIN1 paralogous genes (OsPIN1a-1d) are important for root and panicle development in rice (Oryza sativa L.). However, the specific role of OsPIN1 paralogous genes is still not clear. To understand the specific roles of PIN1 paralogs in rice, we generated pin1 triple and quadruple mutants by cr...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2022.2065432 |
| _version_ | 1827379208455716864 |
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| author | Yong Li Lingling Wu Meiyan Ren Jianshu Zhu Jiming Xu Han Hu Xiaokang Quan Chongping Huang Chuanzao Mao |
| author_facet | Yong Li Lingling Wu Meiyan Ren Jianshu Zhu Jiming Xu Han Hu Xiaokang Quan Chongping Huang Chuanzao Mao |
| author_sort | Yong Li |
| collection | DOAJ |
| description | The OsPIN1 paralogous genes (OsPIN1a-1d) are important for root and panicle development in rice (Oryza sativa L.). However, the specific role of OsPIN1 paralogous genes is still not clear. To understand the specific roles of PIN1 paralogs in rice, we generated pin1 triple and quadruple mutants by crossing the pin1a pin1b and pin1c pin1d double mutants which we previously created. Compared with the 7-day-old wild type, the pin1a pin1c pin1d and pin1b pin1c pin1d triple mutants showed no obvious phenotype variation except that the pin1a pin1c pin1d triple mutant had shorter primary root and shoot. The pin1a pin1b pin1c and pin1a pin1b pin1d triple mutants exhibited a series of developmental abnormalities, including shorter primary roots, longer root hairs, fewer crown roots and lateral roots, shorter and curved shoots. Furthermore, the pin1a pin1b pin1c pin1d quadruple mutant displayed more severe phenotypic defects which was lethal. In addition, the expression levels of some hormone signal transduction and crown root development related genes, such as OsIAAs, OsARFs, OsRRs, and OsCRLs, were significantly altered in the stem base of all examined pin1 multiple mutants. Taken together, our results demonstrated that the four OsPIN1 paralogous genes function redundantly in regulating rice growth and development. |
| first_indexed | 2024-03-08T13:08:51Z |
| format | Article |
| id | doaj.art-fc81ad821f6b41b29c99d675782dadf2 |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:51Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-fc81ad821f6b41b29c99d675782dadf22024-01-18T15:58:21ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242022-12-0117110.1080/15592324.2022.20654322065432Functional redundancy of OsPIN1 paralogous genes in regulating plant growth and development in riceYong Li0Lingling Wu1Meiyan Ren2Jianshu Zhu3Jiming Xu4Han Hu5Xiaokang Quan6Chongping Huang7Chuanzao Mao8Zhejiang UniversityZhejiang UniversityZhejiang UniversityZhejiang UniversityZhejiang UniversityAgricultural Experiment Station of Zhejiang UniversityAgricultural Experiment Station of Zhejiang UniversityAgricultural Experiment Station of Zhejiang UniversityZhejiang UniversityThe OsPIN1 paralogous genes (OsPIN1a-1d) are important for root and panicle development in rice (Oryza sativa L.). However, the specific role of OsPIN1 paralogous genes is still not clear. To understand the specific roles of PIN1 paralogs in rice, we generated pin1 triple and quadruple mutants by crossing the pin1a pin1b and pin1c pin1d double mutants which we previously created. Compared with the 7-day-old wild type, the pin1a pin1c pin1d and pin1b pin1c pin1d triple mutants showed no obvious phenotype variation except that the pin1a pin1c pin1d triple mutant had shorter primary root and shoot. The pin1a pin1b pin1c and pin1a pin1b pin1d triple mutants exhibited a series of developmental abnormalities, including shorter primary roots, longer root hairs, fewer crown roots and lateral roots, shorter and curved shoots. Furthermore, the pin1a pin1b pin1c pin1d quadruple mutant displayed more severe phenotypic defects which was lethal. In addition, the expression levels of some hormone signal transduction and crown root development related genes, such as OsIAAs, OsARFs, OsRRs, and OsCRLs, were significantly altered in the stem base of all examined pin1 multiple mutants. Taken together, our results demonstrated that the four OsPIN1 paralogous genes function redundantly in regulating rice growth and development.http://dx.doi.org/10.1080/15592324.2022.2065432ospin1auxinoryza sativaroot |
| spellingShingle | Yong Li Lingling Wu Meiyan Ren Jianshu Zhu Jiming Xu Han Hu Xiaokang Quan Chongping Huang Chuanzao Mao Functional redundancy of OsPIN1 paralogous genes in regulating plant growth and development in rice Plant Signaling & Behavior ospin1 auxin oryza sativa root |
| title | Functional redundancy of OsPIN1 paralogous genes in regulating plant growth and development in rice |
| title_full | Functional redundancy of OsPIN1 paralogous genes in regulating plant growth and development in rice |
| title_fullStr | Functional redundancy of OsPIN1 paralogous genes in regulating plant growth and development in rice |
| title_full_unstemmed | Functional redundancy of OsPIN1 paralogous genes in regulating plant growth and development in rice |
| title_short | Functional redundancy of OsPIN1 paralogous genes in regulating plant growth and development in rice |
| title_sort | functional redundancy of ospin1 paralogous genes in regulating plant growth and development in rice |
| topic | ospin1 auxin oryza sativa root |
| url | http://dx.doi.org/10.1080/15592324.2022.2065432 |
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