Kinetics of antibody response by Dot-ELISA in rabbits immunized with adult Haemonchus contortus antigen
Whole adult soluble extract of Haemonchus contortus as an antigen along with Freund's complete adjuvant, was used to immunize rabbits. Antisera from immunized rabbits were collected at intervals of 30, 60, 90, 120, 150 and 180 days. For the detection and titration of anti-H. contortus antibodie...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2002-12-01
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| Series: | Parasite |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1051/parasite/2002094363 |
| _version_ | 1797642897789550592 |
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| author | Kaur K. Kapur J. Parmar A. Sood M.L. |
| author_facet | Kaur K. Kapur J. Parmar A. Sood M.L. |
| author_sort | Kaur K. |
| collection | DOAJ |
| description | Whole adult soluble extract of Haemonchus contortus as an antigen along with Freund's complete adjuvant, was used to immunize rabbits. Antisera from immunized rabbits were collected at intervals of 30, 60, 90, 120, 150 and 180 days. For the detection and titration of anti-H. contortus antibodies in these sera, Dot-ELISA was developed. Sera collected 30 days post-immunization exhibited a titre of 1:5,000 in all the rabbits except one, where a titre of 20,000 was recorded. Later, all the rabbits attained the highest titre of 40,000 at different periods of post-immunizations, which were maintained 150-180 days. These high titre sera can be of immense use in the identification and characterization of immunodominant antigens of adult H. contortus. |
| first_indexed | 2024-03-11T14:07:48Z |
| format | Article |
| id | doaj.art-fc81f22403f54b309a09718cd37a98eb |
| institution | Directory Open Access Journal |
| issn | 1252-607X 1776-1042 |
| language | English |
| last_indexed | 2024-03-11T14:07:48Z |
| publishDate | 2002-12-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-fc81f22403f54b309a09718cd37a98eb2023-11-02T01:39:11ZengEDP SciencesParasite1252-607X1776-10422002-12-019436336510.1051/parasite/2002094363parasite2002094p363Kinetics of antibody response by Dot-ELISA in rabbits immunized with adult Haemonchus contortus antigenKaur K.Kapur J.Parmar A.Sood M.L.Whole adult soluble extract of Haemonchus contortus as an antigen along with Freund's complete adjuvant, was used to immunize rabbits. Antisera from immunized rabbits were collected at intervals of 30, 60, 90, 120, 150 and 180 days. For the detection and titration of anti-H. contortus antibodies in these sera, Dot-ELISA was developed. Sera collected 30 days post-immunization exhibited a titre of 1:5,000 in all the rabbits except one, where a titre of 20,000 was recorded. Later, all the rabbits attained the highest titre of 40,000 at different periods of post-immunizations, which were maintained 150-180 days. These high titre sera can be of immense use in the identification and characterization of immunodominant antigens of adult H. contortus.http://dx.doi.org/10.1051/parasite/2002094363Haemonchus contortusDot-ELISAantigenimmune response |
| spellingShingle | Kaur K. Kapur J. Parmar A. Sood M.L. Kinetics of antibody response by Dot-ELISA in rabbits immunized with adult Haemonchus contortus antigen Parasite Haemonchus contortus Dot-ELISA antigen immune response |
| title | Kinetics of antibody response by Dot-ELISA in rabbits immunized with adult Haemonchus contortus antigen |
| title_full | Kinetics of antibody response by Dot-ELISA in rabbits immunized with adult Haemonchus contortus antigen |
| title_fullStr | Kinetics of antibody response by Dot-ELISA in rabbits immunized with adult Haemonchus contortus antigen |
| title_full_unstemmed | Kinetics of antibody response by Dot-ELISA in rabbits immunized with adult Haemonchus contortus antigen |
| title_short | Kinetics of antibody response by Dot-ELISA in rabbits immunized with adult Haemonchus contortus antigen |
| title_sort | kinetics of antibody response by dot elisa in rabbits immunized with adult haemonchus contortus antigen |
| topic | Haemonchus contortus Dot-ELISA antigen immune response |
| url | http://dx.doi.org/10.1051/parasite/2002094363 |
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