The effect of N-acetylcysteine on mechanical fatigue resistance of antibiotic-loaded bone cement
Abstract Background This biomechanical study evaluates the effect of N-acetylcysteine alone and in combination with the most commonly used antibiotic-loaded bone cement mixtures. Methods We mixed eight bone cement mixture groups including combinations of N-acetylcysteine, gentamicin, teicoplanin, an...
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13018-018-0843-9 |
| _version_ | 1811305059652206592 |
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| author | Erhan Sukur Abdulhalim Akar Huseyin Nevzat Topcu Ozgur Cicekli Alauddin Kochai Mehmet Turker |
| author_facet | Erhan Sukur Abdulhalim Akar Huseyin Nevzat Topcu Ozgur Cicekli Alauddin Kochai Mehmet Turker |
| author_sort | Erhan Sukur |
| collection | DOAJ |
| description | Abstract Background This biomechanical study evaluates the effect of N-acetylcysteine alone and in combination with the most commonly used antibiotic-loaded bone cement mixtures. Methods We mixed eight bone cement mixture groups including combinations of N-acetylcysteine, gentamicin, teicoplanin, and vancomycin and applied a four-point bending test individually to each sample on days 1 and 15 using an MTS Acumen test device. Results The result was less than 50 MPa—the limit declared by the ISO (International Standards Organization)—in only the “gentamicin + bone cement + N-acetylcysteine” group. Mechanical fatigue resistance of the bone cement decreased significantly with the addition of N-acetylcysteine both on day 1 and day 15 (p < 0.001). With the addition of N-acetylcysteine into the “gentamicin + bone cement” and “vancomycin + bone cement” mixtures, a significant decrease in mechanical fatigue resistance was observed both on day 1 and day 15 (p < 0.001). In contrast, with the addition of N-acetylcysteine into the “teicoplanin + bone cement” mixture, no significant difference in mechanical fatigue resistance was observed on days 1 and 15 (p = 0.093, p = 0.356). Conclusion Preliminary results indicate that adding N-acetylcysteine to teicoplanin-loaded bone cement does not significantly affect the cement’s mechanical resistance, potentially leading to a new avenue for preventing and treating peri-prosthetic joint infection. N-acetylcysteine may, therefore, be considered as an alternative agent to be added to antibiotic-loaded bone cement mixtures used in the prevention of peri-prosthetic joint infection. |
| first_indexed | 2024-04-13T08:18:35Z |
| format | Article |
| id | doaj.art-fc8203a38e8746e8ba1de0a5c5c399da |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-13T08:18:35Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-fc8203a38e8746e8ba1de0a5c5c399da2022-12-22T02:54:42ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2018-05-011311610.1186/s13018-018-0843-9The effect of N-acetylcysteine on mechanical fatigue resistance of antibiotic-loaded bone cementErhan Sukur0Abdulhalim Akar1Huseyin Nevzat Topcu2Ozgur Cicekli3Alauddin Kochai4Mehmet Turker5Department of Orthopedics and Traumatology, Sakarya University Research and Training HospitalDepartment of Orthopedics and Traumatology, Sakarya University Research and Training HospitalDepartment of Orthopedics and Traumatology, Kocaeli Medical Park HospitalDepartment of Orthopedics and Traumatology, Sakarya University Research and Training HospitalDepartment of Orthopedics and Traumatology, Sakarya University Research and Training HospitalDepartment of Orthopedics and Traumatology, Sakarya University Research and Training HospitalAbstract Background This biomechanical study evaluates the effect of N-acetylcysteine alone and in combination with the most commonly used antibiotic-loaded bone cement mixtures. Methods We mixed eight bone cement mixture groups including combinations of N-acetylcysteine, gentamicin, teicoplanin, and vancomycin and applied a four-point bending test individually to each sample on days 1 and 15 using an MTS Acumen test device. Results The result was less than 50 MPa—the limit declared by the ISO (International Standards Organization)—in only the “gentamicin + bone cement + N-acetylcysteine” group. Mechanical fatigue resistance of the bone cement decreased significantly with the addition of N-acetylcysteine both on day 1 and day 15 (p < 0.001). With the addition of N-acetylcysteine into the “gentamicin + bone cement” and “vancomycin + bone cement” mixtures, a significant decrease in mechanical fatigue resistance was observed both on day 1 and day 15 (p < 0.001). In contrast, with the addition of N-acetylcysteine into the “teicoplanin + bone cement” mixture, no significant difference in mechanical fatigue resistance was observed on days 1 and 15 (p = 0.093, p = 0.356). Conclusion Preliminary results indicate that adding N-acetylcysteine to teicoplanin-loaded bone cement does not significantly affect the cement’s mechanical resistance, potentially leading to a new avenue for preventing and treating peri-prosthetic joint infection. N-acetylcysteine may, therefore, be considered as an alternative agent to be added to antibiotic-loaded bone cement mixtures used in the prevention of peri-prosthetic joint infection.http://link.springer.com/article/10.1186/s13018-018-0843-9N-acetylcysteineAntibiotic-loadedBone cementPeri-prosthetic joint infection |
| spellingShingle | Erhan Sukur Abdulhalim Akar Huseyin Nevzat Topcu Ozgur Cicekli Alauddin Kochai Mehmet Turker The effect of N-acetylcysteine on mechanical fatigue resistance of antibiotic-loaded bone cement Journal of Orthopaedic Surgery and Research N-acetylcysteine Antibiotic-loaded Bone cement Peri-prosthetic joint infection |
| title | The effect of N-acetylcysteine on mechanical fatigue resistance of antibiotic-loaded bone cement |
| title_full | The effect of N-acetylcysteine on mechanical fatigue resistance of antibiotic-loaded bone cement |
| title_fullStr | The effect of N-acetylcysteine on mechanical fatigue resistance of antibiotic-loaded bone cement |
| title_full_unstemmed | The effect of N-acetylcysteine on mechanical fatigue resistance of antibiotic-loaded bone cement |
| title_short | The effect of N-acetylcysteine on mechanical fatigue resistance of antibiotic-loaded bone cement |
| title_sort | effect of n acetylcysteine on mechanical fatigue resistance of antibiotic loaded bone cement |
| topic | N-acetylcysteine Antibiotic-loaded Bone cement Peri-prosthetic joint infection |
| url | http://link.springer.com/article/10.1186/s13018-018-0843-9 |
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