Bovine abortion by a vaccine strain of Bacillus anthracis
ABSTRACT: This paper reports the abortion of a male Aberdeen Angus bovine by a vaccine strain of Bacillus anthracis, describing the pathological and microbiological findings and the genome sequence. Necropsy findings included multifocal areas of hemorrhage in different organs. Histologically, variou...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
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| Series: | Ciência Rural |
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| Online Access: | http://www.scielo.br/pdf/cr/v50n12/1678-4596-cr-50-12-e20200264.pdf |
| _version_ | 1819179156841168896 |
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| author | Daniele Mariath Bassuino Franciele Maboni Siqueira Guilherme Konradt Andréia Vielmo Verônica Machado Rolim Maiara Aline Gonçalves Samuel Paulo Cibulski Gustavo Snel Fabiana Quoos Mayer Agueda Castagna de Vargas David Driemeier Saulo Petinatti Pavarini |
| author_facet | Daniele Mariath Bassuino Franciele Maboni Siqueira Guilherme Konradt Andréia Vielmo Verônica Machado Rolim Maiara Aline Gonçalves Samuel Paulo Cibulski Gustavo Snel Fabiana Quoos Mayer Agueda Castagna de Vargas David Driemeier Saulo Petinatti Pavarini |
| author_sort | Daniele Mariath Bassuino |
| collection | DOAJ |
| description | ABSTRACT: This paper reports the abortion of a male Aberdeen Angus bovine by a vaccine strain of Bacillus anthracis, describing the pathological and microbiological findings and the genome sequence. Necropsy findings included multifocal areas of hemorrhage in different organs. Histologically, various organs showed hemorrhage, fibrin exudation, necrosis associated with countless bacillary bacterial clumps and severe neutrophilic inflammatory infiltrate. In the microbiological examination, numerous rough, nonhemolytic, gray and dry colonies with irregular edges were isolated from liver, lung and abomasum content samples. Gram staining revealed square-ended Gram-positive rods arranged in chains. B. anthracis identification was confirmed by detection of the molecular chromosomal marker Ba813. The genomes from the isolated B. anthracis (named SPV842_15) and from the isolated vaccinal strain (Brazilian vaccinal strain), which was recovered from a commercial vaccine used in the pregnant cow, were sequenced. Genomic comparisons displayed a high level of nucleotide identity in the comparisons between B. anthracis SPV842_15 and the B. anthracis Brazilian vaccinal strain (98,2%). Furthermore, in both strains, only the plasmid pX01 sequence was detected. Although, vaccination against anthrax is characterized by an elevated protective profile and very low residual virulence, immunization with Sterne strains can cause abortion in cattle, presumably by the plasmid pX01 toxins in rare or special situations. |
| first_indexed | 2024-12-22T21:53:58Z |
| format | Article |
| id | doaj.art-fc83b390719f45e3a70d16f4db61c063 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-12-22T21:53:58Z |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-fc83b390719f45e3a70d16f4db61c0632022-12-21T18:11:17ZengUniversidade Federal de Santa MariaCiência Rural1678-459610.1590/0103-8478cr20200264Bovine abortion by a vaccine strain of Bacillus anthracisDaniele Mariath BassuinoFranciele Maboni SiqueiraGuilherme KonradtAndréia VielmoVerônica Machado RolimMaiara Aline GonçalvesSamuel Paulo CibulskiGustavo SnelFabiana Quoos MayerAgueda Castagna de VargasDavid DriemeierSaulo Petinatti PavariniABSTRACT: This paper reports the abortion of a male Aberdeen Angus bovine by a vaccine strain of Bacillus anthracis, describing the pathological and microbiological findings and the genome sequence. Necropsy findings included multifocal areas of hemorrhage in different organs. Histologically, various organs showed hemorrhage, fibrin exudation, necrosis associated with countless bacillary bacterial clumps and severe neutrophilic inflammatory infiltrate. In the microbiological examination, numerous rough, nonhemolytic, gray and dry colonies with irregular edges were isolated from liver, lung and abomasum content samples. Gram staining revealed square-ended Gram-positive rods arranged in chains. B. anthracis identification was confirmed by detection of the molecular chromosomal marker Ba813. The genomes from the isolated B. anthracis (named SPV842_15) and from the isolated vaccinal strain (Brazilian vaccinal strain), which was recovered from a commercial vaccine used in the pregnant cow, were sequenced. Genomic comparisons displayed a high level of nucleotide identity in the comparisons between B. anthracis SPV842_15 and the B. anthracis Brazilian vaccinal strain (98,2%). Furthermore, in both strains, only the plasmid pX01 sequence was detected. Although, vaccination against anthrax is characterized by an elevated protective profile and very low residual virulence, immunization with Sterne strains can cause abortion in cattle, presumably by the plasmid pX01 toxins in rare or special situations.http://www.scielo.br/pdf/cr/v50n12/1678-4596-cr-50-12-e20200264.pdfanthraxcattlefetal lossinfectious diseases |
| spellingShingle | Daniele Mariath Bassuino Franciele Maboni Siqueira Guilherme Konradt Andréia Vielmo Verônica Machado Rolim Maiara Aline Gonçalves Samuel Paulo Cibulski Gustavo Snel Fabiana Quoos Mayer Agueda Castagna de Vargas David Driemeier Saulo Petinatti Pavarini Bovine abortion by a vaccine strain of Bacillus anthracis Ciência Rural anthrax cattle fetal loss infectious diseases |
| title | Bovine abortion by a vaccine strain of Bacillus anthracis |
| title_full | Bovine abortion by a vaccine strain of Bacillus anthracis |
| title_fullStr | Bovine abortion by a vaccine strain of Bacillus anthracis |
| title_full_unstemmed | Bovine abortion by a vaccine strain of Bacillus anthracis |
| title_short | Bovine abortion by a vaccine strain of Bacillus anthracis |
| title_sort | bovine abortion by a vaccine strain of bacillus anthracis |
| topic | anthrax cattle fetal loss infectious diseases |
| url | http://www.scielo.br/pdf/cr/v50n12/1678-4596-cr-50-12-e20200264.pdf |
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