Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae)
The maize weevil, Sitophilus zeamais (Motschulsky), is a major pest of stored grain kernels. Irradiation is an established technique for controlling insects in stored grain and is a major stress factor affecting these insects. Since heat shock protein (hsp) genes respond to this stress, we proposed...
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| Format: | Article |
| Language: | English |
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Institute of Entomology, Biology Centre, Czech Academy of Science
2016-01-01
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| Series: | European Journal of Entomology |
| Subjects: | |
| Online Access: | https://www.eje.cz/artkey/eje-201601-0017_Effects_of_ultraviolet-C_and_microwave_irradiation_on_the_expression_of_heat_shock_protein_genes_in_the_maize_w.php |
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| author | Jatuporn TUNGJITWITAYAKUL Nujira TATUN Boongeua VAJARASATHIRA Sho SAKURAI |
| author_facet | Jatuporn TUNGJITWITAYAKUL Nujira TATUN Boongeua VAJARASATHIRA Sho SAKURAI |
| author_sort | Jatuporn TUNGJITWITAYAKUL |
| collection | DOAJ |
| description | The maize weevil, Sitophilus zeamais (Motschulsky), is a major pest of stored grain kernels. Irradiation is an established technique for controlling insects in stored grain and is a major stress factor affecting these insects. Since heat shock protein (hsp) genes respond to this stress, we proposed that hsps may be associated with irradiated stress tolerance in S. zeamais. The responses of the maize weevil to exposure to ultraviolet-C (UV-C) and microwave irradiation were assessed at four developmental stages: egg, larva, pupa and adult. The results revealed that exposure to UV-C (254 nm, < 8 h) did not affect the survival of the maize weevils; however, Szhsp70, Szhsc70 and Szhsp90 mRNA levels significantly increased during the first 1 h of UV-C exposure. The median lethal time (LT50) of exposure to microwave irradiation indicated that the adult stage was more tolerant of microwave irradiation than the other developmental stages. Microwave irradiation enhanced the expression of the three hsps, but the intensity of up-regulation differed among the three genes, with Szhsp70 the most highly up-regulated. Our experiments revealed that UV-C and microwave irradiation influenced the expression profile of hsp genes in S. zeamais. At the tissue level, the gene responses to UV-C and microwave irradiation varied greatly in different tissues. |
| first_indexed | 2024-12-24T01:19:07Z |
| format | Article |
| id | doaj.art-fc854821ae53409aaf86b75ed83ddeaf |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-24T01:19:07Z |
| publishDate | 2016-01-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-fc854821ae53409aaf86b75ed83ddeaf2022-12-21T17:22:42ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292016-01-01113113514210.14411/eje.2016.017eje-201601-0017Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae)Jatuporn TUNGJITWITAYAKUL0Nujira TATUN1Boongeua VAJARASATHIRA2Sho SAKURAI3School of Science, University of Phayao, Phayao 56000, Thailand; e-mails: jatuporn.tu@up.ac.th, nujira.ta@up.ac.thSchool of Science, University of Phayao, Phayao 56000, Thailand; e-mails: jatuporn.tu@up.ac.th, nujira.ta@up.ac.thDepartment of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand; e-mail: fscibov@ku.ac.thCreative Science Museum, Komatsunomori, Komatsu 923-8610, Japan; e-mail: ssakurai@staff.kanazawa-u.ac.jpThe maize weevil, Sitophilus zeamais (Motschulsky), is a major pest of stored grain kernels. Irradiation is an established technique for controlling insects in stored grain and is a major stress factor affecting these insects. Since heat shock protein (hsp) genes respond to this stress, we proposed that hsps may be associated with irradiated stress tolerance in S. zeamais. The responses of the maize weevil to exposure to ultraviolet-C (UV-C) and microwave irradiation were assessed at four developmental stages: egg, larva, pupa and adult. The results revealed that exposure to UV-C (254 nm, < 8 h) did not affect the survival of the maize weevils; however, Szhsp70, Szhsc70 and Szhsp90 mRNA levels significantly increased during the first 1 h of UV-C exposure. The median lethal time (LT50) of exposure to microwave irradiation indicated that the adult stage was more tolerant of microwave irradiation than the other developmental stages. Microwave irradiation enhanced the expression of the three hsps, but the intensity of up-regulation differed among the three genes, with Szhsp70 the most highly up-regulated. Our experiments revealed that UV-C and microwave irradiation influenced the expression profile of hsp genes in S. zeamais. At the tissue level, the gene responses to UV-C and microwave irradiation varied greatly in different tissues.https://www.eje.cz/artkey/eje-201601-0017_Effects_of_ultraviolet-C_and_microwave_irradiation_on_the_expression_of_heat_shock_protein_genes_in_the_maize_w.phpcoleopteracurculionidaesitophilus zeamaisuv-cmicrowaveheat shock proteinshsp70hsc70hsp90 |
| spellingShingle | Jatuporn TUNGJITWITAYAKUL Nujira TATUN Boongeua VAJARASATHIRA Sho SAKURAI Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae) European Journal of Entomology coleoptera curculionidae sitophilus zeamais uv-c microwave heat shock proteins hsp70 hsc70 hsp90 |
| title | Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae) |
| title_full | Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae) |
| title_fullStr | Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae) |
| title_full_unstemmed | Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae) |
| title_short | Effects of ultraviolet-C and microwave irradiation on the expression of heat shock protein genes in the maize weevil (Coleoptera: Curculionidae) |
| title_sort | effects of ultraviolet c and microwave irradiation on the expression of heat shock protein genes in the maize weevil coleoptera curculionidae |
| topic | coleoptera curculionidae sitophilus zeamais uv-c microwave heat shock proteins hsp70 hsc70 hsp90 |
| url | https://www.eje.cz/artkey/eje-201601-0017_Effects_of_ultraviolet-C_and_microwave_irradiation_on_the_expression_of_heat_shock_protein_genes_in_the_maize_w.php |
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