Mycobacterial Infections in Invasive Turtle Species in Poland
Over the last 30 years, the number of invasive turtle species living in the wild has significantly increased in Poland. This proliferation carries many threats, which mainly include the displacement of native species of animals from their natural habitats. Turtles can also be reservoirs for pathogen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/12/4/570 |
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| author | Łukasz Radulski Monika Krajewska-Wędzina Marek Lipiec Marcin Weiner Anna Zabost Ewa Augustynowicz-Kopeć |
| author_facet | Łukasz Radulski Monika Krajewska-Wędzina Marek Lipiec Marcin Weiner Anna Zabost Ewa Augustynowicz-Kopeć |
| author_sort | Łukasz Radulski |
| collection | DOAJ |
| description | Over the last 30 years, the number of invasive turtle species living in the wild has significantly increased in Poland. This proliferation carries many threats, which mainly include the displacement of native species of animals from their natural habitats. Turtles can also be reservoirs for pathogens, including bacteria from the <i>Mycobacterium</i> genus. In order to confirm or rule out the presence of acid-fast mycobacteria in the population of invasive turtle species, samples from carapace, plastron, internal organs and mouth cavity swabs from 125 animals were tested. Twenty-eight mycobacterial strains were isolated in culture, which were classified as atypical following multiplex-PCR reactions. The GenoType Mycobacterium Common Mycobacteria (CM) test, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, PCR-restriction fragment length polymorphism (PRA)-<i>hsp65</i> and DNA sequencing were used to identify the species of isolates. Of the 28 strains, 11 were identified as <i>M. fortuitum</i>, 10 as <i>M. chelonae</i>, 3 as <i>M. avium</i> ssp. <i>avium</i>, 2 as <i>M. nonchromogenicum</i> and 1 each of <i>M. neoaurum</i> and <i>M. scrofulaceum</i>. The results of the research will also strengthen the understanding that these animals can be vectors for pathogens when living in the wild. |
| first_indexed | 2024-03-11T04:39:55Z |
| format | Article |
| id | doaj.art-fc8bcaf5bcb74b2592d844c762d7d178 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-11T04:39:55Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-fc8bcaf5bcb74b2592d844c762d7d1782023-11-17T20:48:35ZengMDPI AGPathogens2076-08172023-04-0112457010.3390/pathogens12040570Mycobacterial Infections in Invasive Turtle Species in PolandŁukasz Radulski0Monika Krajewska-Wędzina1Marek Lipiec2Marcin Weiner3Anna Zabost4Ewa Augustynowicz-Kopeć5County Veterinary Inspectorate in Puławy, C.K. Norwida 17, 24-100 Puławy, PolandNational Veterinary Research Institute, 24-100 Puławy, PolandNational Veterinary Research Institute, 24-100 Puławy, PolandPope John Paul II State School of Higher Education, Sidorska 95/97, 21-500 Biała Podlaska, PolandDepartment of Microbiology, National Tuberculosis and Lung Diseases Research Institute, 01-138 Warsaw, PolandDepartment of Microbiology, National Tuberculosis and Lung Diseases Research Institute, 01-138 Warsaw, PolandOver the last 30 years, the number of invasive turtle species living in the wild has significantly increased in Poland. This proliferation carries many threats, which mainly include the displacement of native species of animals from their natural habitats. Turtles can also be reservoirs for pathogens, including bacteria from the <i>Mycobacterium</i> genus. In order to confirm or rule out the presence of acid-fast mycobacteria in the population of invasive turtle species, samples from carapace, plastron, internal organs and mouth cavity swabs from 125 animals were tested. Twenty-eight mycobacterial strains were isolated in culture, which were classified as atypical following multiplex-PCR reactions. The GenoType Mycobacterium Common Mycobacteria (CM) test, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, PCR-restriction fragment length polymorphism (PRA)-<i>hsp65</i> and DNA sequencing were used to identify the species of isolates. Of the 28 strains, 11 were identified as <i>M. fortuitum</i>, 10 as <i>M. chelonae</i>, 3 as <i>M. avium</i> ssp. <i>avium</i>, 2 as <i>M. nonchromogenicum</i> and 1 each of <i>M. neoaurum</i> and <i>M. scrofulaceum</i>. The results of the research will also strengthen the understanding that these animals can be vectors for pathogens when living in the wild.https://www.mdpi.com/2076-0817/12/4/570mycobacteriosisinvasive turtle speciesatypical mycobacteria |
| spellingShingle | Łukasz Radulski Monika Krajewska-Wędzina Marek Lipiec Marcin Weiner Anna Zabost Ewa Augustynowicz-Kopeć Mycobacterial Infections in Invasive Turtle Species in Poland Pathogens mycobacteriosis invasive turtle species atypical mycobacteria |
| title | Mycobacterial Infections in Invasive Turtle Species in Poland |
| title_full | Mycobacterial Infections in Invasive Turtle Species in Poland |
| title_fullStr | Mycobacterial Infections in Invasive Turtle Species in Poland |
| title_full_unstemmed | Mycobacterial Infections in Invasive Turtle Species in Poland |
| title_short | Mycobacterial Infections in Invasive Turtle Species in Poland |
| title_sort | mycobacterial infections in invasive turtle species in poland |
| topic | mycobacteriosis invasive turtle species atypical mycobacteria |
| url | https://www.mdpi.com/2076-0817/12/4/570 |
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