Protocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin models
Summary: Glycosylation is one of the most common protein modifications in living organisms and has important regulatory roles in animal tissue development and homeostasis. Here, we present a protocol for generation of 3D organotypic skin models using CRISPR-Cas9 genetically engineered human keratino...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721003750 |
| _version_ | 1819239465768452096 |
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| author | Irina N. Marinova Hans H. Wandall Sally Dabelsteen |
| author_facet | Irina N. Marinova Hans H. Wandall Sally Dabelsteen |
| author_sort | Irina N. Marinova |
| collection | DOAJ |
| description | Summary: Glycosylation is one of the most common protein modifications in living organisms and has important regulatory roles in animal tissue development and homeostasis. Here, we present a protocol for generation of 3D organotypic skin models using CRISPR-Cas9 genetically engineered human keratinocytes (N/TERT-1) to study the role of glycans in epithelial tissue formation. This strategy is also applicable to other gene targets and organotypic tissue models. Careful handling of the cell cultures is critical for the successful formation of the organoids.For complete details on the use and execution of this protocol, please refer to Dabelsteen et al. (2020). |
| first_indexed | 2024-12-23T13:52:33Z |
| format | Article |
| id | doaj.art-fc8cfc673456492c9f0a28d065f3921c |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-23T13:52:33Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-fc8cfc673456492c9f0a28d065f3921c2022-12-21T17:44:33ZengElsevierSTAR Protocols2666-16672021-09-0123100668Protocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin modelsIrina N. Marinova0Hans H. Wandall1Sally Dabelsteen2Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, DenmarkCopenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark; Corresponding authorDepartment of Oral Pathology, School of Dentistry, University of Copenhagen, Copenhagen, Denmark; Corresponding authorSummary: Glycosylation is one of the most common protein modifications in living organisms and has important regulatory roles in animal tissue development and homeostasis. Here, we present a protocol for generation of 3D organotypic skin models using CRISPR-Cas9 genetically engineered human keratinocytes (N/TERT-1) to study the role of glycans in epithelial tissue formation. This strategy is also applicable to other gene targets and organotypic tissue models. Careful handling of the cell cultures is critical for the successful formation of the organoids.For complete details on the use and execution of this protocol, please refer to Dabelsteen et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721003750Cell BiologyCRISPROrganoids |
| spellingShingle | Irina N. Marinova Hans H. Wandall Sally Dabelsteen Protocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin models STAR Protocols Cell Biology CRISPR Organoids |
| title | Protocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin models |
| title_full | Protocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin models |
| title_fullStr | Protocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin models |
| title_full_unstemmed | Protocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin models |
| title_short | Protocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin models |
| title_sort | protocol for crispr cas9 modification of glycosylation in 3d organotypic skin models |
| topic | Cell Biology CRISPR Organoids |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721003750 |
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