Analysis of Narrow-Leaf Lupin Proteins in Lupin-Enriched Pasta by Untargeted and Targeted Mass Spectrometry
The supplementation of different food items with grain legumes and, in particular, with lupin has been demonstrated to provide useful health benefits, especially in the area of cardiovascular disease prevention. In this work, label free quantitative untargeted and targeted approaches based on liquid...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/8/1083 |
| _version_ | 1797559512900567040 |
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| author | Gilda Aiello Yuchen Li Giovanna Boschin Marco Stanziale Carmen Lammi Anna Arnoldi |
| author_facet | Gilda Aiello Yuchen Li Giovanna Boschin Marco Stanziale Carmen Lammi Anna Arnoldi |
| author_sort | Gilda Aiello |
| collection | DOAJ |
| description | The supplementation of different food items with grain legumes and, in particular, with lupin has been demonstrated to provide useful health benefits, especially in the area of cardiovascular disease prevention. In this work, label free quantitative untargeted and targeted approaches based on liquid chromatography−electrospray ionization−tandem mass spectrometry (LC−ESI−MS/MS) for investigating the protein profile of three pasta samples containing different percentages of narrow-leaf lupin flour were carried out. The untargeted method permitted the identification of the main acidic globulins (α-conglutin, β-conglutin, and δ-conglutin) and the comparison of their profile with raw lupin flour. The targeted method, based on High-performance liquid chromatography electrospray ionization tandem mass spectrometry HPLC-Chip-Multiple Reaction Monitoring (MRM) mode, allowed the quantification of γ-conglutin, the main hypoglycemic component of lupin protein: its concentration was around 2.25 mg/g in sample A, 2.16 mg/g in sample D, and 0.57 mg/g in sample F. |
| first_indexed | 2024-03-10T17:46:27Z |
| format | Article |
| id | doaj.art-fc8da2cfcc6546ffb0bf5e47929ea3fe |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T17:46:27Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-fc8da2cfcc6546ffb0bf5e47929ea3fe2023-11-20T09:31:48ZengMDPI AGFoods2304-81582020-08-0198108310.3390/foods9081083Analysis of Narrow-Leaf Lupin Proteins in Lupin-Enriched Pasta by Untargeted and Targeted Mass SpectrometryGilda Aiello0Yuchen Li1Giovanna Boschin2Marco Stanziale3Carmen Lammi4Anna Arnoldi5Department of Human Science and Quality of Life Promotion, Telematic University San Raffaele, 00166 Rome, ItalyDepartment of Pharmaceutical Sciences, University of Milan, 20133 Milan, ItalyDepartment of Pharmaceutical Sciences, University of Milan, 20133 Milan, ItalyDepartment of Research and Development, Rustichella d’Abruzzo S.p.a., 65019 Pianella (PE), ItalyDepartment of Pharmaceutical Sciences, University of Milan, 20133 Milan, ItalyDepartment of Pharmaceutical Sciences, University of Milan, 20133 Milan, ItalyThe supplementation of different food items with grain legumes and, in particular, with lupin has been demonstrated to provide useful health benefits, especially in the area of cardiovascular disease prevention. In this work, label free quantitative untargeted and targeted approaches based on liquid chromatography−electrospray ionization−tandem mass spectrometry (LC−ESI−MS/MS) for investigating the protein profile of three pasta samples containing different percentages of narrow-leaf lupin flour were carried out. The untargeted method permitted the identification of the main acidic globulins (α-conglutin, β-conglutin, and δ-conglutin) and the comparison of their profile with raw lupin flour. The targeted method, based on High-performance liquid chromatography electrospray ionization tandem mass spectrometry HPLC-Chip-Multiple Reaction Monitoring (MRM) mode, allowed the quantification of γ-conglutin, the main hypoglycemic component of lupin protein: its concentration was around 2.25 mg/g in sample A, 2.16 mg/g in sample D, and 0.57 mg/g in sample F.https://www.mdpi.com/2304-8158/9/8/1083food qualityliquid chromatography-tandem mass spectrometry LC-MS/MSfunctional foodslupin-enriched pastalabel free quantificationγ-conglutin |
| spellingShingle | Gilda Aiello Yuchen Li Giovanna Boschin Marco Stanziale Carmen Lammi Anna Arnoldi Analysis of Narrow-Leaf Lupin Proteins in Lupin-Enriched Pasta by Untargeted and Targeted Mass Spectrometry Foods food quality liquid chromatography-tandem mass spectrometry LC-MS/MS functional foods lupin-enriched pasta label free quantification γ-conglutin |
| title | Analysis of Narrow-Leaf Lupin Proteins in Lupin-Enriched Pasta by Untargeted and Targeted Mass Spectrometry |
| title_full | Analysis of Narrow-Leaf Lupin Proteins in Lupin-Enriched Pasta by Untargeted and Targeted Mass Spectrometry |
| title_fullStr | Analysis of Narrow-Leaf Lupin Proteins in Lupin-Enriched Pasta by Untargeted and Targeted Mass Spectrometry |
| title_full_unstemmed | Analysis of Narrow-Leaf Lupin Proteins in Lupin-Enriched Pasta by Untargeted and Targeted Mass Spectrometry |
| title_short | Analysis of Narrow-Leaf Lupin Proteins in Lupin-Enriched Pasta by Untargeted and Targeted Mass Spectrometry |
| title_sort | analysis of narrow leaf lupin proteins in lupin enriched pasta by untargeted and targeted mass spectrometry |
| topic | food quality liquid chromatography-tandem mass spectrometry LC-MS/MS functional foods lupin-enriched pasta label free quantification γ-conglutin |
| url | https://www.mdpi.com/2304-8158/9/8/1083 |
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