The Intriguing mitoNEET: Functional and Spectroscopic Properties of a Unique [2Fe-2S] Cluster Coordination Geometry
Despite the number of cellular and pathological mitoNEET-related processes, very few details are known about the mechanism of action of the protein. The recently discovered existence of a link between NEET proteins and cancer pave the way to consider mitoNEET and its Fe-S clusters as suitable target...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/23/8218 |
| _version_ | 1797462691585982464 |
|---|---|
| author | Francesca Camponeschi Mario Piccioli Lucia Banci |
| author_facet | Francesca Camponeschi Mario Piccioli Lucia Banci |
| author_sort | Francesca Camponeschi |
| collection | DOAJ |
| description | Despite the number of cellular and pathological mitoNEET-related processes, very few details are known about the mechanism of action of the protein. The recently discovered existence of a link between NEET proteins and cancer pave the way to consider mitoNEET and its Fe-S clusters as suitable targets to inhibit cancer cell proliferation. Here, we will review the variety of spectroscopic techniques that have been applied to study mitoNEET in an attempt to explain the drastic difference in clusters stability and reactivity observed for the two redox states, and to elucidate the cellular function of the protein. In particular, the extensive NMR assignment and the characterization of first coordination sphere provide a molecular fingerprint helpful to assist the design of drugs able to impair cellular processes or to directly participate in redox reactions or protein–protein recognition mechanisms. |
| first_indexed | 2024-03-09T17:40:14Z |
| format | Article |
| id | doaj.art-fc8dfe4c80af41018c9beabba27fed2d |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T17:40:14Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-fc8dfe4c80af41018c9beabba27fed2d2023-11-24T11:38:37ZengMDPI AGMolecules1420-30492022-11-012723821810.3390/molecules27238218The Intriguing mitoNEET: Functional and Spectroscopic Properties of a Unique [2Fe-2S] Cluster Coordination GeometryFrancesca Camponeschi0Mario Piccioli1Lucia Banci2Consorzio Internuniversitario Risonanze Magnetiche Metallo Proteine, Via L. Sacconi 6, 50019 Sesto Fiorentino, ItalyConsorzio Internuniversitario Risonanze Magnetiche Metallo Proteine, Via L. Sacconi 6, 50019 Sesto Fiorentino, ItalyConsorzio Internuniversitario Risonanze Magnetiche Metallo Proteine, Via L. Sacconi 6, 50019 Sesto Fiorentino, ItalyDespite the number of cellular and pathological mitoNEET-related processes, very few details are known about the mechanism of action of the protein. The recently discovered existence of a link between NEET proteins and cancer pave the way to consider mitoNEET and its Fe-S clusters as suitable targets to inhibit cancer cell proliferation. Here, we will review the variety of spectroscopic techniques that have been applied to study mitoNEET in an attempt to explain the drastic difference in clusters stability and reactivity observed for the two redox states, and to elucidate the cellular function of the protein. In particular, the extensive NMR assignment and the characterization of first coordination sphere provide a molecular fingerprint helpful to assist the design of drugs able to impair cellular processes or to directly participate in redox reactions or protein–protein recognition mechanisms.https://www.mdpi.com/1420-3049/27/23/8218iron-sulfur proteinsparamagnetic NMRiron-sulfur cluster biogenesisbiophysicshuman pathologiescancer |
| spellingShingle | Francesca Camponeschi Mario Piccioli Lucia Banci The Intriguing mitoNEET: Functional and Spectroscopic Properties of a Unique [2Fe-2S] Cluster Coordination Geometry Molecules iron-sulfur proteins paramagnetic NMR iron-sulfur cluster biogenesis biophysics human pathologies cancer |
| title | The Intriguing mitoNEET: Functional and Spectroscopic Properties of a Unique [2Fe-2S] Cluster Coordination Geometry |
| title_full | The Intriguing mitoNEET: Functional and Spectroscopic Properties of a Unique [2Fe-2S] Cluster Coordination Geometry |
| title_fullStr | The Intriguing mitoNEET: Functional and Spectroscopic Properties of a Unique [2Fe-2S] Cluster Coordination Geometry |
| title_full_unstemmed | The Intriguing mitoNEET: Functional and Spectroscopic Properties of a Unique [2Fe-2S] Cluster Coordination Geometry |
| title_short | The Intriguing mitoNEET: Functional and Spectroscopic Properties of a Unique [2Fe-2S] Cluster Coordination Geometry |
| title_sort | intriguing mitoneet functional and spectroscopic properties of a unique 2fe 2s cluster coordination geometry |
| topic | iron-sulfur proteins paramagnetic NMR iron-sulfur cluster biogenesis biophysics human pathologies cancer |
| url | https://www.mdpi.com/1420-3049/27/23/8218 |
| work_keys_str_mv | AT francescacamponeschi theintriguingmitoneetfunctionalandspectroscopicpropertiesofaunique2fe2sclustercoordinationgeometry AT mariopiccioli theintriguingmitoneetfunctionalandspectroscopicpropertiesofaunique2fe2sclustercoordinationgeometry AT luciabanci theintriguingmitoneetfunctionalandspectroscopicpropertiesofaunique2fe2sclustercoordinationgeometry AT francescacamponeschi intriguingmitoneetfunctionalandspectroscopicpropertiesofaunique2fe2sclustercoordinationgeometry AT mariopiccioli intriguingmitoneetfunctionalandspectroscopicpropertiesofaunique2fe2sclustercoordinationgeometry AT luciabanci intriguingmitoneetfunctionalandspectroscopicpropertiesofaunique2fe2sclustercoordinationgeometry |