Comprehensive analysis of prognostic alternative splicing signature in cervical cancer
Abstract Background Alternative splicing (AS) is a key factor in protein-coding gene diversity, and is associated with the development and progression of malignant tumours. However, the role of AS in cervical cancer is unclear. Methods The AS data for cervical squamous cell carcinoma and endocervica...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | Cancer Cell International |
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| Online Access: | http://link.springer.com/article/10.1186/s12935-020-01299-4 |
| _version_ | 1811212029015359488 |
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| author | Dong Ouyang Ping Yang Jing Cai Si Sun Zehua Wang |
| author_facet | Dong Ouyang Ping Yang Jing Cai Si Sun Zehua Wang |
| author_sort | Dong Ouyang |
| collection | DOAJ |
| description | Abstract Background Alternative splicing (AS) is a key factor in protein-coding gene diversity, and is associated with the development and progression of malignant tumours. However, the role of AS in cervical cancer is unclear. Methods The AS data for cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) were downloaded from The Cancer Genome Atlas (TCGA) SpliceSeq website. Few prognostic AS events were identified through univariate Cox analysis. We further identified the prognostic prediction models of the seven subtypes of AS events and assessed their predictive power. We constructed a clinical prediction model through global analysis of prognostic AS events and established a nomogram using the risk score calculated from the prognostic model and relevant clinical information. Unsupervised cluster analysis was used to explore the relationship between prognostic AS events in the model and clinical features. Results A total of 2860 prognostic AS events in cervical cancer were identified. The best predictive effect was shown by a single alternate acceptor subtype with an area under the curve of 0.96. Our clinical prognostic model included a nine-AS event signature, and the c-index of the predicted nomogram model was 0.764. SNRPA and CCDC12 were hub genes for prognosis-associated splicing factors. Unsupervised cluster analysis through the nine prognostic AS events revealed three clusters with different survival patterns. Conclusions AS events affect the prognosis and biological progression of cervical cancer. The identified prognostic AS events and splicing regulatory networks can increase our understanding of the underlying mechanisms of cervical cancer, providing new therapeutic strategies. |
| first_indexed | 2024-04-12T05:21:57Z |
| format | Article |
| id | doaj.art-fc9171880fb34dd195b5db144924233f |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-04-12T05:21:57Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-fc9171880fb34dd195b5db144924233f2022-12-22T03:46:26ZengBMCCancer Cell International1475-28672020-06-0120111510.1186/s12935-020-01299-4Comprehensive analysis of prognostic alternative splicing signature in cervical cancerDong Ouyang0Ping Yang1Jing Cai2Si Sun3Zehua Wang4Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Obstetrics and Gynecology, First Affiliated Hospital, School of Medicine, Shihezi UniversityDepartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Background Alternative splicing (AS) is a key factor in protein-coding gene diversity, and is associated with the development and progression of malignant tumours. However, the role of AS in cervical cancer is unclear. Methods The AS data for cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) were downloaded from The Cancer Genome Atlas (TCGA) SpliceSeq website. Few prognostic AS events were identified through univariate Cox analysis. We further identified the prognostic prediction models of the seven subtypes of AS events and assessed their predictive power. We constructed a clinical prediction model through global analysis of prognostic AS events and established a nomogram using the risk score calculated from the prognostic model and relevant clinical information. Unsupervised cluster analysis was used to explore the relationship between prognostic AS events in the model and clinical features. Results A total of 2860 prognostic AS events in cervical cancer were identified. The best predictive effect was shown by a single alternate acceptor subtype with an area under the curve of 0.96. Our clinical prognostic model included a nine-AS event signature, and the c-index of the predicted nomogram model was 0.764. SNRPA and CCDC12 were hub genes for prognosis-associated splicing factors. Unsupervised cluster analysis through the nine prognostic AS events revealed three clusters with different survival patterns. Conclusions AS events affect the prognosis and biological progression of cervical cancer. The identified prognostic AS events and splicing regulatory networks can increase our understanding of the underlying mechanisms of cervical cancer, providing new therapeutic strategies.http://link.springer.com/article/10.1186/s12935-020-01299-4ASNomogramPrognosisSplicing factorThe Cancer Genome AtlasCervical cancer |
| spellingShingle | Dong Ouyang Ping Yang Jing Cai Si Sun Zehua Wang Comprehensive analysis of prognostic alternative splicing signature in cervical cancer Cancer Cell International AS Nomogram Prognosis Splicing factor The Cancer Genome Atlas Cervical cancer |
| title | Comprehensive analysis of prognostic alternative splicing signature in cervical cancer |
| title_full | Comprehensive analysis of prognostic alternative splicing signature in cervical cancer |
| title_fullStr | Comprehensive analysis of prognostic alternative splicing signature in cervical cancer |
| title_full_unstemmed | Comprehensive analysis of prognostic alternative splicing signature in cervical cancer |
| title_short | Comprehensive analysis of prognostic alternative splicing signature in cervical cancer |
| title_sort | comprehensive analysis of prognostic alternative splicing signature in cervical cancer |
| topic | AS Nomogram Prognosis Splicing factor The Cancer Genome Atlas Cervical cancer |
| url | http://link.springer.com/article/10.1186/s12935-020-01299-4 |
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