Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins
Phycotoxins and mycotoxins, such as paralytic shellfish poisoning toxins, type A trichothecenes, and aflatoxins are among the most toxic low molecular weight toxins associated with human poisoning incidents through the consumption of naturally contaminated food. Therefore, there is an utmost need fo...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-02-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fchem.2019.00031/full |
| _version_ | 1818214447908388864 |
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| author | Katharina Schulz Katharina Schulz Christopher Pöhlmann Richard Dietrich Erwin Märtlbauer Thomas Elßner |
| author_facet | Katharina Schulz Katharina Schulz Christopher Pöhlmann Richard Dietrich Erwin Märtlbauer Thomas Elßner |
| author_sort | Katharina Schulz |
| collection | DOAJ |
| description | Phycotoxins and mycotoxins, such as paralytic shellfish poisoning toxins, type A trichothecenes, and aflatoxins are among the most toxic low molecular weight toxins associated with human poisoning incidents through the consumption of naturally contaminated food. Therefore, there is an utmost need for rapid and sensitive on-site detection systems. Herein, an electrochemical biochip for fast detection of saxitoxin, T-2 toxin as well as aflatoxin M1 and their corresponding congeners, respectively, using a portable and fully automated detection platform (pBDi, portable BioDetector integrated) was developed. Toxin analysis is facilitated upon the biochip via an indirect competitive immunoassay using toxin-specific antibodies combined with anti-idiotypic antibodies. The developed biochips enable detection in the low ng/mL-range within 17 min. Moreover, the assays cover a wide linear working range of 2–3 orders of magnitude above the limit of detection with an inter-chip coefficient of variation lower than 15%. The broad specificity of the employed antibodies which react with a large number of congeners within the respective toxin group allows efficient screening of contaminated samples for the presence of these low molecular weight toxins. With respect to the analysis of human urine samples, we focused here on the detection of saxitoxin, HT-2 toxin, and aflatoxin M1, all known as biomarkers of acute toxin exposure. Overall, it was proved that the developed biochip assays can be used to rapidly and reliably identify severe intoxications caused by these low molecular weight toxins. |
| first_indexed | 2024-12-12T06:20:20Z |
| format | Article |
| id | doaj.art-fc97849ea9014e318d3b53dc7642f3f4 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-12T06:20:20Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-fc97849ea9014e318d3b53dc7642f3f42022-12-22T00:34:55ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462019-02-01710.3389/fchem.2019.00031435734Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight ToxinsKatharina Schulz0Katharina Schulz1Christopher Pöhlmann2Richard Dietrich3Erwin Märtlbauer4Thomas Elßner5Bruker Daltonik GmbH, Leipzig, GermanyDepartment of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität München, Munich, GermanyBruker Daltonik GmbH, Leipzig, GermanyDepartment of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität München, Munich, GermanyDepartment of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität München, Munich, GermanyBruker Daltonik GmbH, Leipzig, GermanyPhycotoxins and mycotoxins, such as paralytic shellfish poisoning toxins, type A trichothecenes, and aflatoxins are among the most toxic low molecular weight toxins associated with human poisoning incidents through the consumption of naturally contaminated food. Therefore, there is an utmost need for rapid and sensitive on-site detection systems. Herein, an electrochemical biochip for fast detection of saxitoxin, T-2 toxin as well as aflatoxin M1 and their corresponding congeners, respectively, using a portable and fully automated detection platform (pBDi, portable BioDetector integrated) was developed. Toxin analysis is facilitated upon the biochip via an indirect competitive immunoassay using toxin-specific antibodies combined with anti-idiotypic antibodies. The developed biochips enable detection in the low ng/mL-range within 17 min. Moreover, the assays cover a wide linear working range of 2–3 orders of magnitude above the limit of detection with an inter-chip coefficient of variation lower than 15%. The broad specificity of the employed antibodies which react with a large number of congeners within the respective toxin group allows efficient screening of contaminated samples for the presence of these low molecular weight toxins. With respect to the analysis of human urine samples, we focused here on the detection of saxitoxin, HT-2 toxin, and aflatoxin M1, all known as biomarkers of acute toxin exposure. Overall, it was proved that the developed biochip assays can be used to rapidly and reliably identify severe intoxications caused by these low molecular weight toxins.https://www.frontiersin.org/article/10.3389/fchem.2019.00031/fullelectrochemical biochipon-site detectionpBDicompetitive immunoassayanti-idiotypic antibodiesaflatoxins |
| spellingShingle | Katharina Schulz Katharina Schulz Christopher Pöhlmann Richard Dietrich Erwin Märtlbauer Thomas Elßner Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins Frontiers in Chemistry electrochemical biochip on-site detection pBDi competitive immunoassay anti-idiotypic antibodies aflatoxins |
| title | Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins |
| title_full | Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins |
| title_fullStr | Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins |
| title_full_unstemmed | Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins |
| title_short | Electrochemical Biochip Assays Based on Anti-idiotypic Antibodies for Rapid and Automated On-Site Detection of Low Molecular Weight Toxins |
| title_sort | electrochemical biochip assays based on anti idiotypic antibodies for rapid and automated on site detection of low molecular weight toxins |
| topic | electrochemical biochip on-site detection pBDi competitive immunoassay anti-idiotypic antibodies aflatoxins |
| url | https://www.frontiersin.org/article/10.3389/fchem.2019.00031/full |
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