Modification of Substrate Affinity of Nitrile Hydratase Based on Amino Acid Hotspot Mutation

Modification of Substrate Affinity of Nitrile Hydratase Based on Amino Acid Hotspot Mutation

Objective: A semi-rational design was used to increase the affinity of the nitrile hydratase (ReNHase) derived from Rhodococcus erythropolis CCM2595 with the substrate nicotinonitrile. Methods: The 1AHJ protein with high homology was found through sequence comparison and evaluated by software Swiss-...

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Bibliographic Details
Main Authors:	Baocheng CUI, Jiao HUANG, Jiaxin LI, Yi GUO, Li WANG, Changhai LIANG
Format:	Article
Language:	zho
Published:	The editorial department of Science and Technology of Food Industry 2022-04-01
Series:	Shipin gongye ke-ji
Subjects:	nitrile hydratase nicotinonitrile virtual amino acid mutation substrate affinity
Online Access:	http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021080147

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