| Summary: | A novel Gram staining positive, aerobic bacterium NEAU-HV1<sup>T</sup> that exhibits antifungal activity against <i>Exserohilum turcicum</i> was isolated from a soil collected from Gama, Hadjer lamis, Chad. It was grown at 10−45 °C (optimum 30 °C), pH 5−10 (optimum pH 8), and 0−4% (w/v) NaCl (optimum 1%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-HV1<sup>T</sup> was closely related to <i>Sinomonas susongensis</i> A31<sup>T</sup> (99.24% sequence similarity), <i>Sinomonas humi</i> MUSC 117<sup>T</sup> (98.76%), and <i>Sinomonas albida</i> LC13<sup>T</sup> (98.68%). The average nucleotide identity values between NEAU-HV1<sup>T</sup> and its most closely related species were 79.34−85.49%. The digital DNA−DNA hybridization values between NEAU-HV1<sup>T</sup> and <i>S. susongensis</i> A31<sup>T</sup>, <i>S. albida</i> LC13<sup>T</sup>, and <i>S. humi</i> MUSC 117<sup>T</sup> were 23.20, 23.50, and 22.80%, respectively, again indicating that they belonged to different taxa. The genomic DNA G+C content was 67.64 mol%. The whole cell sugars contained galactose, mannose, and rhamnose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and four glycolipids. The respiratory quinone system comprised MK-9(H<sub>2</sub>), MK-10(H<sub>2</sub>), and MK-8(H<sub>2</sub>). The major cellular fatty acids (>5%) were <i>anteiso</i>-C<sub>15:0</sub>, <i>anteiso</i>-C<sub>17:0</sub>, C<sub>16:0</sub>, and <i>iso</i>-C<sub>15:0</sub>. Based on the polyphasic analysis, it is suggested that the strain NEAU-HV1<sup>T</sup> represents a novel species of the genus <i>Sinomonas</i>, for which the name <i>Sinomonas gamaensis</i> sp. nov. is proposed. The type strain is NEAU-HV1<sup>T</sup> (= DSM 104514<sup>T</sup> = CCTCC M 2017246<sup>T</sup>).
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