Characterization of <i>Sinomonas gamaensis</i> sp. nov., a Novel Soil Bacterium with Antifungal Activity against <i>Exserohilum turcicum</i>
A novel Gram staining positive, aerobic bacterium NEAU-HV1<sup>T</sup> that exhibits antifungal activity against <i>Exserohilum turcicum</i> was isolated from a soil collected from Gama, Hadjer lamis, Chad. It was grown at 10−45 °C (optimum 30 °C),...
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MDPI AG
2019-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/6/170 |
| _version_ | 1819242691295182848 |
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| author | Yansong Fu Rui Yan Dongli Liu Junwei Zhao Jia Song Xiangjing Wang Lin Cui Ji Zhang Wensheng Xiang |
| author_facet | Yansong Fu Rui Yan Dongli Liu Junwei Zhao Jia Song Xiangjing Wang Lin Cui Ji Zhang Wensheng Xiang |
| author_sort | Yansong Fu |
| collection | DOAJ |
| description | A novel Gram staining positive, aerobic bacterium NEAU-HV1<sup>T</sup> that exhibits antifungal activity against <i>Exserohilum turcicum</i> was isolated from a soil collected from Gama, Hadjer lamis, Chad. It was grown at 10−45 °C (optimum 30 °C), pH 5−10 (optimum pH 8), and 0−4% (w/v) NaCl (optimum 1%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-HV1<sup>T</sup> was closely related to <i>Sinomonas susongensis</i> A31<sup>T</sup> (99.24% sequence similarity), <i>Sinomonas humi</i> MUSC 117<sup>T</sup> (98.76%), and <i>Sinomonas albida</i> LC13<sup>T</sup> (98.68%). The average nucleotide identity values between NEAU-HV1<sup>T</sup> and its most closely related species were 79.34−85.49%. The digital DNA−DNA hybridization values between NEAU-HV1<sup>T</sup> and <i>S. susongensis</i> A31<sup>T</sup>, <i>S. albida</i> LC13<sup>T</sup>, and <i>S. humi</i> MUSC 117<sup>T</sup> were 23.20, 23.50, and 22.80%, respectively, again indicating that they belonged to different taxa. The genomic DNA G+C content was 67.64 mol%. The whole cell sugars contained galactose, mannose, and rhamnose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and four glycolipids. The respiratory quinone system comprised MK-9(H<sub>2</sub>), MK-10(H<sub>2</sub>), and MK-8(H<sub>2</sub>). The major cellular fatty acids (>5%) were <i>anteiso</i>-C<sub>15:0</sub>, <i>anteiso</i>-C<sub>17:0</sub>, C<sub>16:0</sub>, and <i>iso</i>-C<sub>15:0</sub>. Based on the polyphasic analysis, it is suggested that the strain NEAU-HV1<sup>T</sup> represents a novel species of the genus <i>Sinomonas</i>, for which the name <i>Sinomonas gamaensis</i> sp. nov. is proposed. The type strain is NEAU-HV1<sup>T</sup> (= DSM 104514<sup>T</sup> = CCTCC M 2017246<sup>T</sup>). |
| first_indexed | 2024-12-23T14:43:49Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-12-23T14:43:49Z |
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| series | Microorganisms |
| spelling | doaj.art-fc9bd8847c4f495e9f896fa8858fc2662022-12-21T17:43:09ZengMDPI AGMicroorganisms2076-26072019-06-017617010.3390/microorganisms7060170microorganisms7060170Characterization of <i>Sinomonas gamaensis</i> sp. nov., a Novel Soil Bacterium with Antifungal Activity against <i>Exserohilum turcicum</i>Yansong Fu0Rui Yan1Dongli Liu2Junwei Zhao3Jia Song4Xiangjing Wang5Lin Cui6Ji Zhang7Wensheng Xiang8Heilongjiang Provincial Key Laboratory of Agricultural Microbiology, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Provincial Key Laboratory of Agricultural Microbiology, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Provincial Key Laboratory of Agricultural Microbiology, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Provincial Key Laboratory of Agricultural Microbiology, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Provincial Key Laboratory of Agricultural Microbiology, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Provincial Key Laboratory of Agricultural Microbiology, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Provincial Key Laboratory of Agricultural Microbiology, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Provincial Key Laboratory of Agricultural Microbiology, Northeast Agricultural University, Harbin 150030, ChinaHeilongjiang Provincial Key Laboratory of Agricultural Microbiology, Northeast Agricultural University, Harbin 150030, ChinaA novel Gram staining positive, aerobic bacterium NEAU-HV1<sup>T</sup> that exhibits antifungal activity against <i>Exserohilum turcicum</i> was isolated from a soil collected from Gama, Hadjer lamis, Chad. It was grown at 10−45 °C (optimum 30 °C), pH 5−10 (optimum pH 8), and 0−4% (w/v) NaCl (optimum 1%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-HV1<sup>T</sup> was closely related to <i>Sinomonas susongensis</i> A31<sup>T</sup> (99.24% sequence similarity), <i>Sinomonas humi</i> MUSC 117<sup>T</sup> (98.76%), and <i>Sinomonas albida</i> LC13<sup>T</sup> (98.68%). The average nucleotide identity values between NEAU-HV1<sup>T</sup> and its most closely related species were 79.34−85.49%. The digital DNA−DNA hybridization values between NEAU-HV1<sup>T</sup> and <i>S. susongensis</i> A31<sup>T</sup>, <i>S. albida</i> LC13<sup>T</sup>, and <i>S. humi</i> MUSC 117<sup>T</sup> were 23.20, 23.50, and 22.80%, respectively, again indicating that they belonged to different taxa. The genomic DNA G+C content was 67.64 mol%. The whole cell sugars contained galactose, mannose, and rhamnose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and four glycolipids. The respiratory quinone system comprised MK-9(H<sub>2</sub>), MK-10(H<sub>2</sub>), and MK-8(H<sub>2</sub>). The major cellular fatty acids (>5%) were <i>anteiso</i>-C<sub>15:0</sub>, <i>anteiso</i>-C<sub>17:0</sub>, C<sub>16:0</sub>, and <i>iso</i>-C<sub>15:0</sub>. Based on the polyphasic analysis, it is suggested that the strain NEAU-HV1<sup>T</sup> represents a novel species of the genus <i>Sinomonas</i>, for which the name <i>Sinomonas gamaensis</i> sp. nov. is proposed. The type strain is NEAU-HV1<sup>T</sup> (= DSM 104514<sup>T</sup> = CCTCC M 2017246<sup>T</sup>).https://www.mdpi.com/2076-2607/7/6/170novel species<i>Sinomonas</i>antifungal activity<i>Exserohilum turcicum</i> |
| spellingShingle | Yansong Fu Rui Yan Dongli Liu Junwei Zhao Jia Song Xiangjing Wang Lin Cui Ji Zhang Wensheng Xiang Characterization of <i>Sinomonas gamaensis</i> sp. nov., a Novel Soil Bacterium with Antifungal Activity against <i>Exserohilum turcicum</i> Microorganisms novel species <i>Sinomonas</i> antifungal activity <i>Exserohilum turcicum</i> |
| title | Characterization of <i>Sinomonas gamaensis</i> sp. nov., a Novel Soil Bacterium with Antifungal Activity against <i>Exserohilum turcicum</i> |
| title_full | Characterization of <i>Sinomonas gamaensis</i> sp. nov., a Novel Soil Bacterium with Antifungal Activity against <i>Exserohilum turcicum</i> |
| title_fullStr | Characterization of <i>Sinomonas gamaensis</i> sp. nov., a Novel Soil Bacterium with Antifungal Activity against <i>Exserohilum turcicum</i> |
| title_full_unstemmed | Characterization of <i>Sinomonas gamaensis</i> sp. nov., a Novel Soil Bacterium with Antifungal Activity against <i>Exserohilum turcicum</i> |
| title_short | Characterization of <i>Sinomonas gamaensis</i> sp. nov., a Novel Soil Bacterium with Antifungal Activity against <i>Exserohilum turcicum</i> |
| title_sort | characterization of i sinomonas gamaensis i sp nov a novel soil bacterium with antifungal activity against i exserohilum turcicum i |
| topic | novel species <i>Sinomonas</i> antifungal activity <i>Exserohilum turcicum</i> |
| url | https://www.mdpi.com/2076-2607/7/6/170 |
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