Biotin Deficiency Induces Intestinal Dysbiosis Associated with an Inflammatory Bowel Disease-like Phenotype
Biotin is an essential vitamin and critical cofactor in several metabolic pathways, and its deficiency has been linked to several disorders including inflammatory bowel disease (IBD). We previously reported that biotin deficiency (BD) in mice, whether modeled through intestine-specific deletion of b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/2/264 |
| _version_ | 1797438154667458560 |
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| author | Julianne C. Yang Jonathan P. Jacobs Michael Hwang Subrata Sabui Fengting Liang Hamid M. Said Jonathan Skupsky |
| author_facet | Julianne C. Yang Jonathan P. Jacobs Michael Hwang Subrata Sabui Fengting Liang Hamid M. Said Jonathan Skupsky |
| author_sort | Julianne C. Yang |
| collection | DOAJ |
| description | Biotin is an essential vitamin and critical cofactor in several metabolic pathways, and its deficiency has been linked to several disorders including inflammatory bowel disease (IBD). We previously reported that biotin deficiency (BD) in mice, whether modeled through intestine-specific deletion of biotin transporter (SMVT-icKO) or through a biotin-deficient diet, resulted in intestinal inflammation consistent with an IBD-like phenotype. To assess whether the gut microbiome is associated with these BD-induced changes, we collected stool and intestinal samples from both of these mouse models and utilized them for 16S rRNA gene sequencing. We find that both diet-mediated and deletion-mediated BD result in the expansion of opportunistic microbes including <i>Klebsiella</i>, <i>Enterobacter</i>, and <i>Helicobacter</i>, at the expense of mucus-resident microbes including <i>Akkermansia</i>. Additionally, microbiome dysbiosis resulting from diet-mediated BD precedes the onset of the IBD-like phenotypic changes. Lastly, through the use of predictive metagenomics, we report that the resulting BD-linked microbiome perturbations exhibit increased biotin biosynthesis in addition to several other perturbed metabolic pathways. Altogether, these results demonstrate that biotin deficiency results in a specific microbiome composition, which may favor microbes capable of biotin synthesis and which may contribute to intestinal inflammation. |
| first_indexed | 2024-03-09T11:33:08Z |
| format | Article |
| id | doaj.art-fca008c94bb942bdabf4b1423e910ff4 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T11:33:08Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-fca008c94bb942bdabf4b1423e910ff42023-11-30T23:49:08ZengMDPI AGNutrients2072-66432023-01-0115226410.3390/nu15020264Biotin Deficiency Induces Intestinal Dysbiosis Associated with an Inflammatory Bowel Disease-like PhenotypeJulianne C. Yang0Jonathan P. Jacobs1Michael Hwang2Subrata Sabui3Fengting Liang4Hamid M. Said5Jonathan Skupsky6The Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USAThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USADepartment of Physiology and Biophysics, University of California, Irvine, CA 92697, USADepartment of Physiology and Biophysics, University of California, Irvine, CA 92697, USAThe Vatche and Tamar Manoukian Division of Digestive Diseases, Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USADepartment of Physiology and Biophysics, University of California, Irvine, CA 92697, USADepartment of Medicine, University of California, Irvine, CA 92697, USABiotin is an essential vitamin and critical cofactor in several metabolic pathways, and its deficiency has been linked to several disorders including inflammatory bowel disease (IBD). We previously reported that biotin deficiency (BD) in mice, whether modeled through intestine-specific deletion of biotin transporter (SMVT-icKO) or through a biotin-deficient diet, resulted in intestinal inflammation consistent with an IBD-like phenotype. To assess whether the gut microbiome is associated with these BD-induced changes, we collected stool and intestinal samples from both of these mouse models and utilized them for 16S rRNA gene sequencing. We find that both diet-mediated and deletion-mediated BD result in the expansion of opportunistic microbes including <i>Klebsiella</i>, <i>Enterobacter</i>, and <i>Helicobacter</i>, at the expense of mucus-resident microbes including <i>Akkermansia</i>. Additionally, microbiome dysbiosis resulting from diet-mediated BD precedes the onset of the IBD-like phenotypic changes. Lastly, through the use of predictive metagenomics, we report that the resulting BD-linked microbiome perturbations exhibit increased biotin biosynthesis in addition to several other perturbed metabolic pathways. Altogether, these results demonstrate that biotin deficiency results in a specific microbiome composition, which may favor microbes capable of biotin synthesis and which may contribute to intestinal inflammation.https://www.mdpi.com/2072-6643/15/2/264microbiomebiotinIBDcolitis |
| spellingShingle | Julianne C. Yang Jonathan P. Jacobs Michael Hwang Subrata Sabui Fengting Liang Hamid M. Said Jonathan Skupsky Biotin Deficiency Induces Intestinal Dysbiosis Associated with an Inflammatory Bowel Disease-like Phenotype Nutrients microbiome biotin IBD colitis |
| title | Biotin Deficiency Induces Intestinal Dysbiosis Associated with an Inflammatory Bowel Disease-like Phenotype |
| title_full | Biotin Deficiency Induces Intestinal Dysbiosis Associated with an Inflammatory Bowel Disease-like Phenotype |
| title_fullStr | Biotin Deficiency Induces Intestinal Dysbiosis Associated with an Inflammatory Bowel Disease-like Phenotype |
| title_full_unstemmed | Biotin Deficiency Induces Intestinal Dysbiosis Associated with an Inflammatory Bowel Disease-like Phenotype |
| title_short | Biotin Deficiency Induces Intestinal Dysbiosis Associated with an Inflammatory Bowel Disease-like Phenotype |
| title_sort | biotin deficiency induces intestinal dysbiosis associated with an inflammatory bowel disease like phenotype |
| topic | microbiome biotin IBD colitis |
| url | https://www.mdpi.com/2072-6643/15/2/264 |
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