DEVELOPMENT AND EVALUATION OF AN INOCULUM OF CELULOLYTIC BACTERIA
In Colombia, the inappropriate management of solid waste continues generating serious problems related with soil, water, and air contamination. The objective of this research was to generate an inoculum of cellulolytic bacteria and evaluate its effectiveness for the transformation of organic solid w...
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| Format: | Article |
| Language: | Spanish |
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Universidad de Ciencias Aplicadas y Ambientales
2015-06-01
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| Series: | Revista U.D.C.A Actualidad & Divulgación Científica |
| Subjects: | |
| Online Access: | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0123-42262015000100024&lng=en&tlng=en |
| _version_ | 1818684140108644352 |
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| author | Paola Andrea Viteri Flórez David Arturo Castillo Guerra Silvio Edgar Viteri Rosero |
| author_facet | Paola Andrea Viteri Flórez David Arturo Castillo Guerra Silvio Edgar Viteri Rosero |
| author_sort | Paola Andrea Viteri Flórez |
| collection | DOAJ |
| description | In Colombia, the inappropriate management of solid waste continues generating serious problems related with soil, water, and air contamination. The objective of this research was to generate an inoculum of cellulolytic bacteria and evaluate its effectiveness for the transformation of organic solid waste. The study included soil samples from five native forests and five cereal producing farms and from three compost piles. From each sample a serial dilution until 10-4 was prepared. From each soil dilution test tubes containing liquid mineral medium and a strip of filter paper were inoculated. From the filter paper strips which showed zones of degradation the microorganisms were streaked, first on Agar Nutrient and then on solid mineral medium, supplemented with cellulose. The bacteria isolates obtained were evaluated for their cellulolytic capacity and those which showed the highest capacity tested for growth compatibility. In total, 20 isolates of cellulolytic bacteria were obtained, 19 from the native forests and one from a compost pile. Not all the isolates showed equal cellulolytic capacity, 5 of them did not show a halo of hydrolysis of cellulose and in the other 15 the amplitude of the halo varied between 0.1 and 0.7cm. Based on the cellulolytic capacity and compatibility for growth the isolates 1 (Bacillus sp.), 6 (Pseudomonas sp.), and 7 (Erwinia sp.) were selected to generate the inoculum. The inoculation of the compost piles with the selected cellulolytic bacteria or with the EM® and Agroplux® microorganisms did not produce effects statistically different to those naturally produced by the native microorganisms in the composting process. |
| first_indexed | 2024-12-17T10:45:54Z |
| format | Article |
| id | doaj.art-fca02754cd4a41a2a05a2270bc2a07e8 |
| institution | Directory Open Access Journal |
| issn | 0123-4226 |
| language | Spanish |
| last_indexed | 2024-12-17T10:45:54Z |
| publishDate | 2015-06-01 |
| publisher | Universidad de Ciencias Aplicadas y Ambientales |
| record_format | Article |
| series | Revista U.D.C.A Actualidad & Divulgación Científica |
| spelling | doaj.art-fca02754cd4a41a2a05a2270bc2a07e82022-12-21T21:52:07ZspaUniversidad de Ciencias Aplicadas y AmbientalesRevista U.D.C.A Actualidad & Divulgación Científica0123-42262015-06-01181207215S0123-42262015000100024DEVELOPMENT AND EVALUATION OF AN INOCULUM OF CELULOLYTIC BACTERIAPaola Andrea Viteri Flórez0David Arturo Castillo Guerra1Silvio Edgar Viteri Rosero2Universidad Pedagógica y Tecnológica de ColombiaUniversidad Pedagógica y Tecnológica de ColombiaUniversidad Pedagógica y Tecnológica de ColombiaIn Colombia, the inappropriate management of solid waste continues generating serious problems related with soil, water, and air contamination. The objective of this research was to generate an inoculum of cellulolytic bacteria and evaluate its effectiveness for the transformation of organic solid waste. The study included soil samples from five native forests and five cereal producing farms and from three compost piles. From each sample a serial dilution until 10-4 was prepared. From each soil dilution test tubes containing liquid mineral medium and a strip of filter paper were inoculated. From the filter paper strips which showed zones of degradation the microorganisms were streaked, first on Agar Nutrient and then on solid mineral medium, supplemented with cellulose. The bacteria isolates obtained were evaluated for their cellulolytic capacity and those which showed the highest capacity tested for growth compatibility. In total, 20 isolates of cellulolytic bacteria were obtained, 19 from the native forests and one from a compost pile. Not all the isolates showed equal cellulolytic capacity, 5 of them did not show a halo of hydrolysis of cellulose and in the other 15 the amplitude of the halo varied between 0.1 and 0.7cm. Based on the cellulolytic capacity and compatibility for growth the isolates 1 (Bacillus sp.), 6 (Pseudomonas sp.), and 7 (Erwinia sp.) were selected to generate the inoculum. The inoculation of the compost piles with the selected cellulolytic bacteria or with the EM® and Agroplux® microorganisms did not produce effects statistically different to those naturally produced by the native microorganisms in the composting process.http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0123-42262015000100024&lng=en&tlng=enCelulosamicroorganismos celulolíticosbiodegradacióndescomposición |
| spellingShingle | Paola Andrea Viteri Flórez David Arturo Castillo Guerra Silvio Edgar Viteri Rosero DEVELOPMENT AND EVALUATION OF AN INOCULUM OF CELULOLYTIC BACTERIA Revista U.D.C.A Actualidad & Divulgación Científica Celulosa microorganismos celulolíticos biodegradación descomposición |
| title | DEVELOPMENT AND EVALUATION OF AN INOCULUM OF CELULOLYTIC BACTERIA |
| title_full | DEVELOPMENT AND EVALUATION OF AN INOCULUM OF CELULOLYTIC BACTERIA |
| title_fullStr | DEVELOPMENT AND EVALUATION OF AN INOCULUM OF CELULOLYTIC BACTERIA |
| title_full_unstemmed | DEVELOPMENT AND EVALUATION OF AN INOCULUM OF CELULOLYTIC BACTERIA |
| title_short | DEVELOPMENT AND EVALUATION OF AN INOCULUM OF CELULOLYTIC BACTERIA |
| title_sort | development and evaluation of an inoculum of celulolytic bacteria |
| topic | Celulosa microorganismos celulolíticos biodegradación descomposición |
| url | http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0123-42262015000100024&lng=en&tlng=en |
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