Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice
Avian influenza viruses can be efficiently transmitted through mucous membranes, and conventional vaccines are not effective in protecting against mucosal infection by influenza viruses. To induce multiple immune responses in an organism, we constructed a recombinant Lactobacillus plantarum expressi...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-12-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.800965/full |
| _version_ | 1819260415237947392 |
|---|---|
| author | Hui Niu Jun-Hong Xing Bo-Shi Zou Chun-Wei Shi Hai-Bin Huang Yan-Long Jiang Jian-Zhong Wang Xin Cao Nan Wang Yan Zeng Wen-Tao Yang Gui-Lian Yang Chun-Feng Wang |
| author_facet | Hui Niu Jun-Hong Xing Bo-Shi Zou Chun-Wei Shi Hai-Bin Huang Yan-Long Jiang Jian-Zhong Wang Xin Cao Nan Wang Yan Zeng Wen-Tao Yang Gui-Lian Yang Chun-Feng Wang |
| author_sort | Hui Niu |
| collection | DOAJ |
| description | Avian influenza viruses can be efficiently transmitted through mucous membranes, and conventional vaccines are not effective in protecting against mucosal infection by influenza viruses. To induce multiple immune responses in an organism, we constructed a recombinant Lactobacillus plantarum expressing the influenza virus antigen HA1 with the adjuvant dendritic cell-targeting peptide (DCpep). The recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep were used to immunize mice via oral administration, and the humoral, cellular and mucosal immune responses were evaluated. In addition, the serum levels of specific antibodies and hemagglutination inhibition (HI) levels were also measured. Our results showed that recombinant L. plantarum activated dendritic cells in Peyer’s patches (PPs), increased the numbers of CD4+IFN-γ+ and CD8+IFN-γ+ cells in the spleen and mesenteric lymph nodes (MLNs), and affected the ability of CD4+ and CD8+ cells to proliferate in the spleen and MLNs. Additionally, recombinant L. plantarum increased the number of B220+IgA+ cells in PPs and the level of IgA in the lungs and different intestinal segments. In addition, specific IgG, IgG1 and IgG2a antibodies were induced at high levels in the mice serum, specific IgA antibodies were induced at high levels in the mice feces, and HI potency was significantly increased. Thus, the recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep have potential as vaccine candidates for avian influenza virus. |
| first_indexed | 2024-12-23T19:25:32Z |
| format | Article |
| id | doaj.art-fca2780671694d1db8d0d54057b11d6a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-23T19:25:32Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-fca2780671694d1db8d0d54057b11d6a2022-12-21T17:34:03ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-12-011210.3389/fimmu.2021.800965800965Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in MiceHui NiuJun-Hong XingBo-Shi ZouChun-Wei ShiHai-Bin HuangYan-Long JiangJian-Zhong WangXin CaoNan WangYan ZengWen-Tao YangGui-Lian YangChun-Feng WangAvian influenza viruses can be efficiently transmitted through mucous membranes, and conventional vaccines are not effective in protecting against mucosal infection by influenza viruses. To induce multiple immune responses in an organism, we constructed a recombinant Lactobacillus plantarum expressing the influenza virus antigen HA1 with the adjuvant dendritic cell-targeting peptide (DCpep). The recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep were used to immunize mice via oral administration, and the humoral, cellular and mucosal immune responses were evaluated. In addition, the serum levels of specific antibodies and hemagglutination inhibition (HI) levels were also measured. Our results showed that recombinant L. plantarum activated dendritic cells in Peyer’s patches (PPs), increased the numbers of CD4+IFN-γ+ and CD8+IFN-γ+ cells in the spleen and mesenteric lymph nodes (MLNs), and affected the ability of CD4+ and CD8+ cells to proliferate in the spleen and MLNs. Additionally, recombinant L. plantarum increased the number of B220+IgA+ cells in PPs and the level of IgA in the lungs and different intestinal segments. In addition, specific IgG, IgG1 and IgG2a antibodies were induced at high levels in the mice serum, specific IgA antibodies were induced at high levels in the mice feces, and HI potency was significantly increased. Thus, the recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep have potential as vaccine candidates for avian influenza virus.https://www.frontiersin.org/articles/10.3389/fimmu.2021.800965/fullH7N9 avian influenzaHA1 proteinDCpepLactobacillus plantarumimmune response |
| spellingShingle | Hui Niu Jun-Hong Xing Bo-Shi Zou Chun-Wei Shi Hai-Bin Huang Yan-Long Jiang Jian-Zhong Wang Xin Cao Nan Wang Yan Zeng Wen-Tao Yang Gui-Lian Yang Chun-Feng Wang Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice Frontiers in Immunology H7N9 avian influenza HA1 protein DCpep Lactobacillus plantarum immune response |
| title | Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice |
| title_full | Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice |
| title_fullStr | Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice |
| title_full_unstemmed | Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice |
| title_short | Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice |
| title_sort | immune evaluation of recombinant lactobacillus plantarum with surface display of ha1 dcpep in mice |
| topic | H7N9 avian influenza HA1 protein DCpep Lactobacillus plantarum immune response |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.800965/full |
| work_keys_str_mv | AT huiniu immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT junhongxing immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT boshizou immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT chunweishi immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT haibinhuang immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT yanlongjiang immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT jianzhongwang immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT xincao immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT nanwang immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT yanzeng immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT wentaoyang immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT guilianyang immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice AT chunfengwang immuneevaluationofrecombinantlactobacillusplantarumwithsurfacedisplayofha1dcpepinmice |