Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome
Abstract Purpose Antioxidant status and oxidative stress markers in human follicular fluid (FF) surrounding oocytes may be related to the outcomes of in vitro fertilization and embryo transfer (IVF‐ET). Therefore, we herein examined the relationship between antioxidant status and oxidative stress ma...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-10-01
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| Series: | Reproductive Medicine and Biology |
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| Online Access: | https://doi.org/10.1002/rmb2.12229 |
| _version_ | 1819038068726824960 |
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| author | Takuji Nishihara Kazuya Matsumoto Yoshihiko Hosoi Yoshiharu Morimoto |
| author_facet | Takuji Nishihara Kazuya Matsumoto Yoshihiko Hosoi Yoshiharu Morimoto |
| author_sort | Takuji Nishihara |
| collection | DOAJ |
| description | Abstract Purpose Antioxidant status and oxidative stress markers in human follicular fluid (FF) surrounding oocytes may be related to the outcomes of in vitro fertilization and embryo transfer (IVF‐ET). Therefore, we herein examined the relationship between antioxidant status and oxidative stress markers in FF and the outcomes of IVF‐ET. Methods One hundred and seventeen infertile women were included in this study. FF was obtained from mature follicles at the time of oocyte retrieval. The total antioxidant capacity (TAC) and total glutathione (GSH), vitamin C, and 8‐ hydroxy‐2′‐deoxyguanosine (8‐OHdG) concentrations were measured. Results Total GSH levels were lower in patients who had a low fertilization rate after intracytoplasmic sperm injection (ICSI). In addition, 8‐OHdG levels were higher in patients who had a low fertilization rate after ICSI and low rate of good quality blastocysts. Total GSH activity was lower in patients with endometriosis. No significant differences were noted in pregnancy outcomes. Conclusions Total GSH and 8‐OHdG in human FF may be potential markers for fertilization in ART. Also, our findings may suggest that oxidative stress in women with infertility is associated with endometriosis. |
| first_indexed | 2024-12-21T08:31:26Z |
| format | Article |
| id | doaj.art-fca5123a1ecf44a39118b47b97a62fde |
| institution | Directory Open Access Journal |
| issn | 1445-5781 1447-0578 |
| language | English |
| last_indexed | 2024-12-21T08:31:26Z |
| publishDate | 2018-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Reproductive Medicine and Biology |
| spelling | doaj.art-fca5123a1ecf44a39118b47b97a62fde2022-12-21T19:10:12ZengWileyReproductive Medicine and Biology1445-57811447-05782018-10-0117448148610.1002/rmb2.12229Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcomeTakuji Nishihara0Kazuya Matsumoto1Yoshihiko Hosoi2Yoshiharu Morimoto3HORAC Grand Front Osaka Clinic Osaka JapanGraduate School of Biology‐Oriented Science and Technology Kindai University Wakayama JapanGraduate School of Biology‐Oriented Science and Technology Kindai University Wakayama JapanHORAC Grand Front Osaka Clinic Osaka JapanAbstract Purpose Antioxidant status and oxidative stress markers in human follicular fluid (FF) surrounding oocytes may be related to the outcomes of in vitro fertilization and embryo transfer (IVF‐ET). Therefore, we herein examined the relationship between antioxidant status and oxidative stress markers in FF and the outcomes of IVF‐ET. Methods One hundred and seventeen infertile women were included in this study. FF was obtained from mature follicles at the time of oocyte retrieval. The total antioxidant capacity (TAC) and total glutathione (GSH), vitamin C, and 8‐ hydroxy‐2′‐deoxyguanosine (8‐OHdG) concentrations were measured. Results Total GSH levels were lower in patients who had a low fertilization rate after intracytoplasmic sperm injection (ICSI). In addition, 8‐OHdG levels were higher in patients who had a low fertilization rate after ICSI and low rate of good quality blastocysts. Total GSH activity was lower in patients with endometriosis. No significant differences were noted in pregnancy outcomes. Conclusions Total GSH and 8‐OHdG in human FF may be potential markers for fertilization in ART. Also, our findings may suggest that oxidative stress in women with infertility is associated with endometriosis.https://doi.org/10.1002/rmb2.12229eight‐hydroxydeoxyguanosinefollicular fluidglutathionein vitro fertilizationoxidative stress |
| spellingShingle | Takuji Nishihara Kazuya Matsumoto Yoshihiko Hosoi Yoshiharu Morimoto Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome Reproductive Medicine and Biology eight‐hydroxydeoxyguanosine follicular fluid glutathione in vitro fertilization oxidative stress |
| title | Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome |
| title_full | Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome |
| title_fullStr | Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome |
| title_full_unstemmed | Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome |
| title_short | Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome |
| title_sort | evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome |
| topic | eight‐hydroxydeoxyguanosine follicular fluid glutathione in vitro fertilization oxidative stress |
| url | https://doi.org/10.1002/rmb2.12229 |
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