Anti-Obesity Activities of Standardized <i>Ecklonia stolonifera</i> Extract in 3T3-L1 Preadipocytes and High-Fat-Diet-Fed ICR Mice
The purpose of this study was to prepare a clinical trial test material (ESETM, test material of <i>Ecklonia stolonifera</i> extract) to develop a health functional food based on its anti-obesity effect. The anti-obesity effect of ESETM was evaluated in 3T3-L1 adipocytes and obese mice f...
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MDPI AG
2022-05-01
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| Online Access: | https://www.mdpi.com/2076-3417/12/10/5115 |
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| author | Xionggao Han Sun-Il Choi Xiao Men Se-Jeong Lee Heegu Jin Hyun-Ji Oh Eunjin Kim Jongwook Kim Boo-Yong Lee Ok-Hwan Lee |
| author_facet | Xionggao Han Sun-Il Choi Xiao Men Se-Jeong Lee Heegu Jin Hyun-Ji Oh Eunjin Kim Jongwook Kim Boo-Yong Lee Ok-Hwan Lee |
| author_sort | Xionggao Han |
| collection | DOAJ |
| description | The purpose of this study was to prepare a clinical trial test material (ESETM, test material of <i>Ecklonia stolonifera</i> extract) to develop a health functional food based on its anti-obesity effect. The anti-obesity effect of ESETM was evaluated in 3T3-L1 adipocytes and obese mice fed a high-fat diet (HFD) to confirm its nonclinical trial effect before application in clinical trial. Adipogenesis is a process of preadipocyte differentiation that causes an increase in the production of reactive oxygen species (ROS) and lipid accumulation. In vitro study results indicated that ESETM outstandingly inhibits the production of ROS and lipid accumulation during adipogenesis and lipogenesis. In vivo, ESETM-treated ICR mice had reduced HFD-induced weight change, food efficiency ratio, adipose tissue weight, liver weight and showed improved serum lipid profiles. Our results show that ESETM inhibits weight change by regulating the adipogenesis, lipogenesis, lipolysis, and thermogenesis pathways. |
| first_indexed | 2024-03-10T03:23:58Z |
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| id | doaj.art-fca57775c8424545a4f3c54f3c12ff17 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T03:23:58Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-fca57775c8424545a4f3c54f3c12ff172023-11-23T09:57:56ZengMDPI AGApplied Sciences2076-34172022-05-011210511510.3390/app12105115Anti-Obesity Activities of Standardized <i>Ecklonia stolonifera</i> Extract in 3T3-L1 Preadipocytes and High-Fat-Diet-Fed ICR MiceXionggao Han0Sun-Il Choi1Xiao Men2Se-Jeong Lee3Heegu Jin4Hyun-Ji Oh5Eunjin Kim6Jongwook Kim7Boo-Yong Lee8Ok-Hwan Lee9Department of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, KoreaDepartment of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam 13488, KoreaDepartment of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam 13488, KoreaNaturalway Co., Ltd., Pocheon 11160, KoreaNaturalway Co., Ltd., Pocheon 11160, KoreaDepartment of Food Science and Biotechnology, College of Life Science, CHA University, Seongnam 13488, KoreaDepartment of Food Biotechnology and Environmental Science, Kangwon National University, Chuncheon 24341, KoreaThe purpose of this study was to prepare a clinical trial test material (ESETM, test material of <i>Ecklonia stolonifera</i> extract) to develop a health functional food based on its anti-obesity effect. The anti-obesity effect of ESETM was evaluated in 3T3-L1 adipocytes and obese mice fed a high-fat diet (HFD) to confirm its nonclinical trial effect before application in clinical trial. Adipogenesis is a process of preadipocyte differentiation that causes an increase in the production of reactive oxygen species (ROS) and lipid accumulation. In vitro study results indicated that ESETM outstandingly inhibits the production of ROS and lipid accumulation during adipogenesis and lipogenesis. In vivo, ESETM-treated ICR mice had reduced HFD-induced weight change, food efficiency ratio, adipose tissue weight, liver weight and showed improved serum lipid profiles. Our results show that ESETM inhibits weight change by regulating the adipogenesis, lipogenesis, lipolysis, and thermogenesis pathways.https://www.mdpi.com/2076-3417/12/10/5115<i>Ecklonia stolonifera</i>obesityclinical trial test materialadipogenesislipolysisbrowning |
| spellingShingle | Xionggao Han Sun-Il Choi Xiao Men Se-Jeong Lee Heegu Jin Hyun-Ji Oh Eunjin Kim Jongwook Kim Boo-Yong Lee Ok-Hwan Lee Anti-Obesity Activities of Standardized <i>Ecklonia stolonifera</i> Extract in 3T3-L1 Preadipocytes and High-Fat-Diet-Fed ICR Mice Applied Sciences <i>Ecklonia stolonifera</i> obesity clinical trial test material adipogenesis lipolysis browning |
| title | Anti-Obesity Activities of Standardized <i>Ecklonia stolonifera</i> Extract in 3T3-L1 Preadipocytes and High-Fat-Diet-Fed ICR Mice |
| title_full | Anti-Obesity Activities of Standardized <i>Ecklonia stolonifera</i> Extract in 3T3-L1 Preadipocytes and High-Fat-Diet-Fed ICR Mice |
| title_fullStr | Anti-Obesity Activities of Standardized <i>Ecklonia stolonifera</i> Extract in 3T3-L1 Preadipocytes and High-Fat-Diet-Fed ICR Mice |
| title_full_unstemmed | Anti-Obesity Activities of Standardized <i>Ecklonia stolonifera</i> Extract in 3T3-L1 Preadipocytes and High-Fat-Diet-Fed ICR Mice |
| title_short | Anti-Obesity Activities of Standardized <i>Ecklonia stolonifera</i> Extract in 3T3-L1 Preadipocytes and High-Fat-Diet-Fed ICR Mice |
| title_sort | anti obesity activities of standardized i ecklonia stolonifera i extract in 3t3 l1 preadipocytes and high fat diet fed icr mice |
| topic | <i>Ecklonia stolonifera</i> obesity clinical trial test material adipogenesis lipolysis browning |
| url | https://www.mdpi.com/2076-3417/12/10/5115 |
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