Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro.
Calcium is the key macromineral having a role in skeletal structure and function, muscle contraction, and neurotransmission. Bone remodeling is maintained through a constant balance between calcium resorption and deposition. Calcium deficiency is resolved through calcium supplementation, and among t...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0222240 |
| _version_ | 1818584204983664640 |
|---|---|
| author | Prashant Kumar Modi Ashwini Prabhu Yashodhar P Bhandary Sudheer Shenoy P Aparna Hegde Sindhu Priya Es Renjith P Johnson Shankar Prasad Das Sahil Vazirally Punchappady-Devasya Rekha |
| author_facet | Prashant Kumar Modi Ashwini Prabhu Yashodhar P Bhandary Sudheer Shenoy P Aparna Hegde Sindhu Priya Es Renjith P Johnson Shankar Prasad Das Sahil Vazirally Punchappady-Devasya Rekha |
| author_sort | Prashant Kumar Modi |
| collection | DOAJ |
| description | Calcium is the key macromineral having a role in skeletal structure and function, muscle contraction, and neurotransmission. Bone remodeling is maintained through a constant balance between calcium resorption and deposition. Calcium deficiency is resolved through calcium supplementation, and among the supplements, water-soluble organic molecules attracted great pharmaceutical interest. Calcium glucoheptonate is a highly water-soluble organic calcium salt having clinical use; however, detailed investigations on its biological effects are limited. We assessed the effects of calcium glucoheptonate on cell viability and proliferation of osteoblast-like MG-63 cells. Calcium uptake and mineralization were evaluated using Alizarin red staining of osteoblast-like MG-63 cells treated with calcium glucoheptonate. Expression of osteogenic markers were monitored by western blotting, immunofluorescence, and qRT-PCR assays. Increased proliferation and calcium uptake were observed in the MG-63 cells treated with calcium glucoheptonate. The treatment also increased the expression of osteopontin and osteogenic genes such as collagen-1, secreted protein acidic and cysteine rich (SPARC), and osteocalcin. Calcium glucoheptonate treatment did not exert any cytotoxicity on colorectal and renal epithelial cells, indicating the safety of the treatment. This is the first report with evidence for its beneficial effect for pharmaceutical use in addressing calcium deficiency conditions. |
| first_indexed | 2024-12-16T08:17:28Z |
| format | Article |
| id | doaj.art-fca6128e911f44cab075bfd7f043b8bb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T08:17:28Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fca6128e911f44cab075bfd7f043b8bb2022-12-21T22:38:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01149e022224010.1371/journal.pone.0222240Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro.Prashant Kumar ModiAshwini PrabhuYashodhar P BhandarySudheer Shenoy PAparna HegdeSindhu Priya EsRenjith P JohnsonShankar Prasad DasSahil VazirallyPunchappady-Devasya RekhaCalcium is the key macromineral having a role in skeletal structure and function, muscle contraction, and neurotransmission. Bone remodeling is maintained through a constant balance between calcium resorption and deposition. Calcium deficiency is resolved through calcium supplementation, and among the supplements, water-soluble organic molecules attracted great pharmaceutical interest. Calcium glucoheptonate is a highly water-soluble organic calcium salt having clinical use; however, detailed investigations on its biological effects are limited. We assessed the effects of calcium glucoheptonate on cell viability and proliferation of osteoblast-like MG-63 cells. Calcium uptake and mineralization were evaluated using Alizarin red staining of osteoblast-like MG-63 cells treated with calcium glucoheptonate. Expression of osteogenic markers were monitored by western blotting, immunofluorescence, and qRT-PCR assays. Increased proliferation and calcium uptake were observed in the MG-63 cells treated with calcium glucoheptonate. The treatment also increased the expression of osteopontin and osteogenic genes such as collagen-1, secreted protein acidic and cysteine rich (SPARC), and osteocalcin. Calcium glucoheptonate treatment did not exert any cytotoxicity on colorectal and renal epithelial cells, indicating the safety of the treatment. This is the first report with evidence for its beneficial effect for pharmaceutical use in addressing calcium deficiency conditions.https://doi.org/10.1371/journal.pone.0222240 |
| spellingShingle | Prashant Kumar Modi Ashwini Prabhu Yashodhar P Bhandary Sudheer Shenoy P Aparna Hegde Sindhu Priya Es Renjith P Johnson Shankar Prasad Das Sahil Vazirally Punchappady-Devasya Rekha Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro. PLoS ONE |
| title | Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro. |
| title_full | Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro. |
| title_fullStr | Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro. |
| title_full_unstemmed | Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro. |
| title_short | Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro. |
| title_sort | effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast like cells in vitro |
| url | https://doi.org/10.1371/journal.pone.0222240 |
| work_keys_str_mv | AT prashantkumarmodi effectofcalciumglucoheptonateonproliferationandosteogenesisofosteoblastlikecellsinvitro AT ashwiniprabhu effectofcalciumglucoheptonateonproliferationandosteogenesisofosteoblastlikecellsinvitro AT yashodharpbhandary effectofcalciumglucoheptonateonproliferationandosteogenesisofosteoblastlikecellsinvitro AT sudheershenoyp effectofcalciumglucoheptonateonproliferationandosteogenesisofosteoblastlikecellsinvitro AT aparnahegde effectofcalciumglucoheptonateonproliferationandosteogenesisofosteoblastlikecellsinvitro AT sindhupriyaes effectofcalciumglucoheptonateonproliferationandosteogenesisofosteoblastlikecellsinvitro AT renjithpjohnson effectofcalciumglucoheptonateonproliferationandosteogenesisofosteoblastlikecellsinvitro AT shankarprasaddas effectofcalciumglucoheptonateonproliferationandosteogenesisofosteoblastlikecellsinvitro AT sahilvazirally effectofcalciumglucoheptonateonproliferationandosteogenesisofosteoblastlikecellsinvitro AT punchappadydevasyarekha effectofcalciumglucoheptonateonproliferationandosteogenesisofosteoblastlikecellsinvitro |